Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5767 and Pf1N1B4_5768 are separated by 1 nucleotides Pf1N1B4_5768 and Pf1N1B4_5769 overlap by 4 nucleotides Pf1N1B4_5769 and Pf1N1B4_5770 overlap by 8 nucleotides Pf1N1B4_5770 and Pf1N1B4_5771 overlap by 26 nucleotides
Pf1N1B4_5767 - Heme d1 biosynthesis protein NirF, at 205,503 to 206,681
_5767
Pf1N1B4_5768 - Heme d1 biosynthesis protein NirD, at 206,683 to 207,141
_5768
Pf1N1B4_5769 - Heme d1 biosynthesis protein NirL, at 207,138 to 207,653
_5769
Pf1N1B4_5770 - Heme d1 biosynthesis protein NirG, at 207,646 to 208,089
_5770
Pf1N1B4_5771 - Heme d1 biosynthesis protein NirH, at 208,064 to 208,579
_5771
Position (kb) 207
208 Strain fitness (log2 ratio) -2
-1
0
1
2 at 206.756 kb on - strand, within Pf1N1B4_5768 at 206.756 kb on - strand, within Pf1N1B4_5768 at 206.964 kb on + strand, within Pf1N1B4_5768 at 206.965 kb on - strand, within Pf1N1B4_5768 at 206.968 kb on + strand at 206.968 kb on + strand, within Pf1N1B4_5768 at 206.969 kb on - strand, within Pf1N1B4_5768 at 206.987 kb on - strand, within Pf1N1B4_5768 at 207.344 kb on + strand, within Pf1N1B4_5769 at 207.344 kb on + strand, within Pf1N1B4_5769 at 207.439 kb on - strand, within Pf1N1B4_5769 at 207.472 kb on - strand, within Pf1N1B4_5769 at 207.929 kb on - strand, within Pf1N1B4_5770 at 207.929 kb on - strand, within Pf1N1B4_5770 at 208.060 kb on + strand at 208.060 kb on + strand at 208.061 kb on - strand at 208.076 kb on - strand at 208.284 kb on - strand, within Pf1N1B4_5771 at 208.548 kb on + strand at 208.618 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 206,756 - Pf1N1B4_5768 0.16 +0.2 206,756 - Pf1N1B4_5768 0.16 +0.5 206,964 + Pf1N1B4_5768 0.61 +1.3 206,965 - Pf1N1B4_5768 0.61 -2.0 206,968 + +0.4 206,968 + Pf1N1B4_5768 0.62 -1.8 206,969 - Pf1N1B4_5768 0.62 -1.2 206,987 - Pf1N1B4_5768 0.66 +0.5 207,344 + Pf1N1B4_5769 0.40 -0.2 207,344 + Pf1N1B4_5769 0.40 -2.5 207,439 - Pf1N1B4_5769 0.58 -0.9 207,472 - Pf1N1B4_5769 0.65 -0.8 207,929 - Pf1N1B4_5770 0.64 -2.2 207,929 - Pf1N1B4_5770 0.64 +2.1 208,060 + -0.6 208,060 + +0.3 208,061 - -0.8 208,076 - +0.5 208,284 - Pf1N1B4_5771 0.43 -0.5 208,548 + -0.3 208,618 + -0.2
Or see this region's nucleotide sequence