Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5678 and Pf1N1B4_5679 are separated by 179 nucleotides Pf1N1B4_5679 and Pf1N1B4_5680 overlap by 4 nucleotides
Pf1N1B4_5678 - sensory box histidine kinase/response regulator, at 114,552 to 116,615
_5678
Pf1N1B4_5679 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 116,795 to 119,872
_5679
Pf1N1B4_5680 - DNA polymerase-like protein PA0670, at 119,869 to 121,284
_5680
Position (kb)
116
117
118
119
120 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 115.867 kb on + strand at 115.906 kb on + strand, within Pf1N1B4_5678 at 115.906 kb on + strand at 116.260 kb on + strand, within Pf1N1B4_5678 at 116.260 kb on + strand at 116.261 kb on - strand, within Pf1N1B4_5678 at 116.422 kb on + strand at 116.453 kb on - strand at 116.463 kb on - strand at 116.464 kb on + strand at 116.464 kb on + strand at 116.464 kb on + strand at 116.473 kb on + strand at 116.474 kb on - strand at 116.523 kb on + strand at 116.633 kb on + strand at 116.633 kb on + strand at 116.775 kb on + strand at 116.775 kb on + strand at 116.778 kb on - strand at 116.778 kb on - strand at 116.933 kb on + strand at 117.094 kb on + strand at 117.214 kb on + strand, within Pf1N1B4_5679 at 117.615 kb on + strand at 117.615 kb on + strand, within Pf1N1B4_5679 at 117.615 kb on + strand, within Pf1N1B4_5679 at 117.693 kb on + strand, within Pf1N1B4_5679 at 117.694 kb on - strand, within Pf1N1B4_5679 at 117.738 kb on + strand at 117.739 kb on - strand, within Pf1N1B4_5679 at 117.965 kb on - strand, within Pf1N1B4_5679 at 117.965 kb on - strand at 117.965 kb on - strand, within Pf1N1B4_5679 at 118.000 kb on - strand, within Pf1N1B4_5679 at 118.021 kb on + strand, within Pf1N1B4_5679 at 118.288 kb on + strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.660 kb on - strand, within Pf1N1B4_5679 at 118.713 kb on - strand, within Pf1N1B4_5679 at 118.713 kb on - strand, within Pf1N1B4_5679 at 118.716 kb on + strand, within Pf1N1B4_5679 at 118.716 kb on + strand, within Pf1N1B4_5679 at 118.717 kb on - strand, within Pf1N1B4_5679 at 118.851 kb on + strand, within Pf1N1B4_5679 at 118.852 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.557 kb on - strand, within Pf1N1B4_5679 at 119.667 kb on - strand at 119.960 kb on + strand at 119.961 kb on - strand at 119.964 kb on - strand at 120.098 kb on - strand at 120.098 kb on - strand, within Pf1N1B4_5680 at 120.098 kb on - strand, within Pf1N1B4_5680 at 120.256 kb on + strand, within Pf1N1B4_5680 at 120.594 kb on - strand, within Pf1N1B4_5680 at 120.594 kb on - strand, within Pf1N1B4_5680 at 120.616 kb on + strand, within Pf1N1B4_5680 at 120.675 kb on + strand, within Pf1N1B4_5680 at 120.675 kb on + strand, within Pf1N1B4_5680 at 120.676 kb on - strand, within Pf1N1B4_5680 at 120.818 kb on - strand at 120.818 kb on - strand, within Pf1N1B4_5680
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 115,867 + -0.8 115,906 + Pf1N1B4_5678 0.66 +1.1 115,906 + +1.8 116,260 + Pf1N1B4_5678 0.83 +0.3 116,260 + -0.8 116,261 - Pf1N1B4_5678 0.83 -0.8 116,422 + +0.2 116,453 - -1.9 116,463 - -0.6 116,464 + +0.3 116,464 + -0.4 116,464 + -0.8 116,473 + +0.2 116,474 - -0.6 116,523 + -0.8 116,633 + -0.9 116,633 + -1.8 116,775 + -0.8 116,775 + -1.2 116,778 - -1.2 116,778 - -0.2 116,933 + -0.8 117,094 + -1.1 117,214 + Pf1N1B4_5679 0.14 -2.0 117,615 + -0.2 117,615 + Pf1N1B4_5679 0.27 -1.2 117,615 + Pf1N1B4_5679 0.27 -1.4 117,693 + Pf1N1B4_5679 0.29 +0.1 117,694 - Pf1N1B4_5679 0.29 -1.2 117,738 + -0.2 117,739 - Pf1N1B4_5679 0.31 -1.4 117,965 - Pf1N1B4_5679 0.38 -2.5 117,965 - +0.4 117,965 - Pf1N1B4_5679 0.38 +0.1 118,000 - Pf1N1B4_5679 0.39 -2.5 118,021 + Pf1N1B4_5679 0.40 +0.1 118,288 + Pf1N1B4_5679 0.49 -0.5 118,660 - Pf1N1B4_5679 0.61 -0.8 118,660 - Pf1N1B4_5679 0.61 -0.9 118,660 - Pf1N1B4_5679 0.61 +0.4 118,660 - Pf1N1B4_5679 0.61 -0.3 118,660 - Pf1N1B4_5679 0.61 -0.6 118,713 - Pf1N1B4_5679 0.62 -1.2 118,713 - Pf1N1B4_5679 0.62 -2.5 118,716 + Pf1N1B4_5679 0.62 -1.2 118,716 + Pf1N1B4_5679 0.62 +0.5 118,717 - Pf1N1B4_5679 0.62 -2.0 118,851 + Pf1N1B4_5679 0.67 -0.5 118,852 - Pf1N1B4_5679 0.67 +0.8 119,418 - Pf1N1B4_5679 0.85 -1.3 119,418 - Pf1N1B4_5679 0.85 +0.8 119,418 - Pf1N1B4_5679 0.85 -1.5 119,557 - Pf1N1B4_5679 0.90 -1.5 119,667 - -0.3 119,960 + -1.2 119,961 - -1.2 119,964 - +0.8 120,098 - -1.2 120,098 - Pf1N1B4_5680 0.16 -0.1 120,098 - Pf1N1B4_5680 0.16 -1.5 120,256 + Pf1N1B4_5680 0.27 -0.8 120,594 - Pf1N1B4_5680 0.51 -0.8 120,594 - Pf1N1B4_5680 0.51 -1.5 120,616 + Pf1N1B4_5680 0.53 -2.2 120,675 + Pf1N1B4_5680 0.57 +0.2 120,675 + Pf1N1B4_5680 0.57 -0.8 120,676 - Pf1N1B4_5680 0.57 +0.2 120,818 - -0.8 120,818 - Pf1N1B4_5680 0.67 -0.5
Or see this region's nucleotide sequence