Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_565 and Pf1N1B4_566 are separated by 128 nucleotides Pf1N1B4_566 and Pf1N1B4_567 are separated by 83 nucleotides Pf1N1B4_567 and Pf1N1B4_568 are separated by 133 nucleotides Pf1N1B4_568 and Pf1N1B4_569 overlap by 4 nucleotides
Pf1N1B4_565 - Fatty acid desaturase, at 661,519 to 662,460
_565
Pf1N1B4_566 - hypothetical protein, at 662,589 to 662,717
_566
Pf1N1B4_567 - Histone acetyltransferase HPA2 and related acetyltransferases, at 662,801 to 663,220
_567
Pf1N1B4_568 - Peptide deformylase (EC 3.5.1.88), at 663,354 to 663,893
_568
Pf1N1B4_569 - Ribonuclease BN (EC 3.1.-.-), at 663,890 to 664,834
_569
Position (kb) 662
663
664 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 661.841 kb on + strand, within Pf1N1B4_565 at 661.911 kb on + strand, within Pf1N1B4_565 at 662.088 kb on - strand at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.354 kb on + strand, within Pf1N1B4_565 at 662.355 kb on - strand, within Pf1N1B4_565 at 662.355 kb on - strand, within Pf1N1B4_565 at 662.355 kb on - strand, within Pf1N1B4_565 at 662.450 kb on + strand at 662.524 kb on - strand at 662.524 kb on - strand at 662.532 kb on - strand at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand, within Pf1N1B4_566 at 662.702 kb on + strand at 662.702 kb on + strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand, within Pf1N1B4_566 at 662.703 kb on - strand at 662.703 kb on - strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.706 kb on + strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.707 kb on - strand at 662.717 kb on + strand at 662.726 kb on + strand at 662.726 kb on + strand at 662.726 kb on + strand at 662.726 kb on + strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.727 kb on - strand at 662.736 kb on + strand at 662.736 kb on + strand at 662.736 kb on + strand at 662.737 kb on - strand at 662.737 kb on - strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.738 kb on + strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.739 kb on - strand at 662.744 kb on + strand at 662.745 kb on - strand at 662.745 kb on - strand at 662.766 kb on + strand at 662.767 kb on - strand at 662.767 kb on - strand at 662.768 kb on + strand at 662.768 kb on + strand at 662.769 kb on - strand at 662.769 kb on - strand at 662.769 kb on - strand at 662.816 kb on + strand at 662.816 kb on + strand at 662.816 kb on + strand at 662.817 kb on - strand at 662.817 kb on - strand at 662.943 kb on + strand at 662.943 kb on + strand, within Pf1N1B4_567 at 662.943 kb on + strand, within Pf1N1B4_567 at 662.943 kb on + strand, within Pf1N1B4_567 at 663.102 kb on - strand at 663.102 kb on - strand, within Pf1N1B4_567 at 663.102 kb on - strand, within Pf1N1B4_567 at 663.118 kb on + strand, within Pf1N1B4_567 at 663.119 kb on - strand, within Pf1N1B4_567 at 663.277 kb on - strand at 663.439 kb on - strand, within Pf1N1B4_568 at 664.060 kb on + strand, within Pf1N1B4_569 at 664.061 kb on - strand at 664.061 kb on - strand, within Pf1N1B4_569 at 664.062 kb on + strand, within Pf1N1B4_569 at 664.063 kb on - strand, within Pf1N1B4_569 at 664.063 kb on - strand, within Pf1N1B4_569 at 664.063 kb on - strand, within Pf1N1B4_569 at 664.063 kb on - strand at 664.063 kb on - strand, within Pf1N1B4_569 at 664.069 kb on - strand, within Pf1N1B4_569 at 664.104 kb on + strand, within Pf1N1B4_569 at 664.104 kb on + strand at 664.105 kb on - strand, within Pf1N1B4_569 at 664.105 kb on - strand, within Pf1N1B4_569 at 664.117 kb on - strand, within Pf1N1B4_569 at 664.117 kb on - strand at 664.137 kb on + strand, within Pf1N1B4_569 at 664.137 kb on + strand at 664.138 kb on - strand, within Pf1N1B4_569 at 664.202 kb on - strand, within Pf1N1B4_569
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 661,841 + Pf1N1B4_565 0.34 +0.2 661,911 + Pf1N1B4_565 0.42 -0.1 662,088 - +1.1 662,354 + Pf1N1B4_565 0.89 +3.1 662,354 + Pf1N1B4_565 0.89 +0.1 662,354 + Pf1N1B4_565 0.89 +3.1 662,354 + Pf1N1B4_565 0.89 -0.7 662,354 + Pf1N1B4_565 0.89 -0.2 662,355 - Pf1N1B4_565 0.89 +1.7 662,355 - Pf1N1B4_565 0.89 +0.3 662,355 - Pf1N1B4_565 0.89 +0.3 662,450 + -0.6 662,524 - -2.5 662,524 - -1.7 662,532 - -0.5 662,702 + Pf1N1B4_566 0.88 +0.5 662,702 + Pf1N1B4_566 0.88 -1.2 662,702 + Pf1N1B4_566 0.88 -0.4 662,702 + Pf1N1B4_566 0.88 +0.1 662,702 + Pf1N1B4_566 0.88 -1.6 662,702 + Pf1N1B4_566 0.88 +0.5 662,702 + Pf1N1B4_566 0.88 +1.1 662,702 + Pf1N1B4_566 0.88 -1.2 662,702 + Pf1N1B4_566 0.88 -0.0 662,702 + +4.1 662,702 + Pf1N1B4_566 0.88 -0.9 662,702 + Pf1N1B4_566 0.88 -1.5 662,702 + +0.1 662,702 + Pf1N1B4_566 0.88 -0.2 662,703 - Pf1N1B4_566 0.88 -0.5 662,703 - Pf1N1B4_566 0.88 -1.4 662,703 - Pf1N1B4_566 0.88 +0.3 662,703 - Pf1N1B4_566 0.88 +1.4 662,703 - Pf1N1B4_566 0.88 -1.7 662,703 - Pf1N1B4_566 0.88 -1.4 662,703 - Pf1N1B4_566 0.88 -0.0 662,703 - Pf1N1B4_566 0.88 -0.5 662,703 - Pf1N1B4_566 0.88 -0.9 662,703 - -0.9 662,703 - -0.5 662,706 + -1.2 662,706 + +1.1 662,706 + -0.0 662,706 + -0.2 662,706 + -0.0 662,707 - +0.3 662,707 - -2.1 662,707 - +2.1 662,707 - -1.0 662,707 - -0.8 662,707 - -1.2 662,707 - +1.1 662,707 - -2.5 662,707 - -1.5 662,717 + -0.5 662,726 + -0.1 662,726 + -0.7 662,726 + -1.5 662,726 + +0.1 662,727 - +2.1 662,727 - +0.5 662,727 - -2.8 662,727 - +0.8 662,727 - +0.5 662,727 - -0.3 662,727 - -1.5 662,727 - -0.5 662,727 - +0.5 662,727 - +1.7 662,736 + +1.6 662,736 + -0.7 662,736 + +0.1 662,737 - -0.5 662,737 - +2.7 662,738 + -1.7 662,738 + -0.7 662,738 + +0.2 662,738 + -1.8 662,738 + +0.1 662,738 + +0.1 662,738 + +2.1 662,738 + -0.5 662,738 + -0.3 662,738 + +1.1 662,739 - -1.5 662,739 - +0.2 662,739 - +0.3 662,739 - +0.4 662,739 - -1.2 662,739 - -0.1 662,739 - -1.7 662,739 - +0.1 662,739 - -1.7 662,739 - -1.1 662,739 - -0.2 662,739 - -1.9 662,739 - -0.6 662,739 - +0.3 662,739 - +2.3 662,739 - +0.0 662,739 - -0.3 662,739 - +0.8 662,739 - -1.7 662,739 - +0.3 662,739 - -1.1 662,739 - -0.7 662,739 - -2.2 662,744 + -1.1 662,745 - -1.7 662,745 - -0.9 662,766 + +1.1 662,767 - -0.1 662,767 - -1.1 662,768 + +0.1 662,768 + -0.6 662,769 - +0.8 662,769 - +0.5 662,769 - +0.8 662,816 + +1.5 662,816 + -0.5 662,816 + -2.2 662,817 - +1.1 662,817 - -0.5 662,943 + +0.5 662,943 + Pf1N1B4_567 0.34 +0.8 662,943 + Pf1N1B4_567 0.34 -0.5 662,943 + Pf1N1B4_567 0.34 -1.7 663,102 - +0.5 663,102 - Pf1N1B4_567 0.72 -2.4 663,102 - Pf1N1B4_567 0.72 -2.2 663,118 + Pf1N1B4_567 0.75 -1.7 663,119 - Pf1N1B4_567 0.76 +0.4 663,277 - -0.5 663,439 - Pf1N1B4_568 0.16 +0.1 664,060 + Pf1N1B4_569 0.18 -1.8 664,061 - -0.5 664,061 - Pf1N1B4_569 0.18 -0.9 664,062 + Pf1N1B4_569 0.18 +0.0 664,063 - Pf1N1B4_569 0.18 +0.1 664,063 - Pf1N1B4_569 0.18 -1.7 664,063 - Pf1N1B4_569 0.18 -0.9 664,063 - +2.7 664,063 - Pf1N1B4_569 0.18 -0.2 664,069 - Pf1N1B4_569 0.19 +0.1 664,104 + Pf1N1B4_569 0.23 -0.9 664,104 + -0.5 664,105 - Pf1N1B4_569 0.23 -1.7 664,105 - Pf1N1B4_569 0.23 +0.1 664,117 - Pf1N1B4_569 0.24 -1.5 664,117 - +0.1 664,137 + Pf1N1B4_569 0.26 -0.5 664,137 + -0.5 664,138 - Pf1N1B4_569 0.26 -0.9 664,202 - Pf1N1B4_569 0.33 +1.1
Or see this region's nucleotide sequence