Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5645 and Pf1N1B4_5646 overlap by 6 nucleotides Pf1N1B4_5646 and Pf1N1B4_5647 are separated by 197 nucleotides Pf1N1B4_5647 and Pf1N1B4_5648 are separated by 133 nucleotides Pf1N1B4_5648 and Pf1N1B4_5649 are separated by 77 nucleotides
Pf1N1B4_5645 - Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2), at 74,291 to 75,136
_5645
Pf1N1B4_5646 - hypothetical protein, at 75,131 to 75,280
_5646
Pf1N1B4_5647 - hypothetical protein, at 75,478 to 75,927
_5647
Pf1N1B4_5648 - transcriptional regulator, MerR family, at 76,061 to 76,465
_5648
Pf1N1B4_5649 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 76,543 to 77,766
_5649
Position (kb) 76
77 Strain fitness (log2 ratio) -2
-1
0
1 at 75.188 kb on - strand, within Pf1N1B4_5646 at 75.577 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.626 kb on - strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.647 kb on - strand, within Pf1N1B4_5647 at 75.712 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 76.062 kb on + strand at 76.062 kb on + strand at 76.505 kb on - strand at 76.669 kb on + strand, within Pf1N1B4_5649 at 77.320 kb on + strand at 77.320 kb on + strand, within Pf1N1B4_5649 at 77.380 kb on + strand at 77.381 kb on - strand, within Pf1N1B4_5649 at 77.381 kb on - strand, within Pf1N1B4_5649
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 75,188 - Pf1N1B4_5646 0.38 -1.2 75,577 + Pf1N1B4_5647 0.22 -1.8 75,625 + Pf1N1B4_5647 0.33 -0.5 75,625 + Pf1N1B4_5647 0.33 -1.2 75,625 + Pf1N1B4_5647 0.33 +1.1 75,626 - Pf1N1B4_5647 0.33 -1.0 75,627 + Pf1N1B4_5647 0.33 +0.8 75,627 + Pf1N1B4_5647 0.33 +1.1 75,627 + Pf1N1B4_5647 0.33 +1.4 75,627 + Pf1N1B4_5647 0.33 -0.8 75,627 + Pf1N1B4_5647 0.33 -0.5 75,647 - Pf1N1B4_5647 0.38 +0.8 75,712 + Pf1N1B4_5647 0.52 +0.5 75,869 + Pf1N1B4_5647 0.87 -0.4 75,869 + Pf1N1B4_5647 0.87 -0.2 76,062 + +1.1 76,062 + -1.0 76,505 - +0.6 76,669 + Pf1N1B4_5649 0.10 -0.2 77,320 + +0.8 77,320 + Pf1N1B4_5649 0.63 +0.6 77,380 + +0.8 77,381 - Pf1N1B4_5649 0.68 -0.2 77,381 - Pf1N1B4_5649 0.68 -2.5
Or see this region's nucleotide sequence