Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5519 and Pf1N1B4_5520 are separated by 485 nucleotides
Pf1N1B4_5519 - hypothetical protein, at 272,494 to 273,657
_5519
Pf1N1B4_5520 - General secretion pathway protein E, at 274,143 to 275,993
_5520
Position (kb) 274
275
276 Strain fitness (log2 ratio) -2
-1
0
1
2 at 273.225 kb on + strand, within Pf1N1B4_5519 at 273.245 kb on + strand, within Pf1N1B4_5519 at 273.406 kb on - strand, within Pf1N1B4_5519 at 273.679 kb on - strand at 273.791 kb on - strand at 273.861 kb on - strand at 274.735 kb on - strand, within Pf1N1B4_5520 at 274.830 kb on + strand, within Pf1N1B4_5520 at 274.831 kb on - strand, within Pf1N1B4_5520 at 274.831 kb on - strand, within Pf1N1B4_5520 at 274.897 kb on - strand, within Pf1N1B4_5520 at 274.900 kb on + strand, within Pf1N1B4_5520 at 274.900 kb on + strand, within Pf1N1B4_5520 at 274.904 kb on + strand, within Pf1N1B4_5520 at 274.904 kb on + strand at 275.023 kb on + strand, within Pf1N1B4_5520 at 275.024 kb on - strand, within Pf1N1B4_5520 at 275.108 kb on - strand, within Pf1N1B4_5520 at 275.108 kb on - strand at 275.179 kb on - strand, within Pf1N1B4_5520 at 275.220 kb on + strand, within Pf1N1B4_5520 at 275.373 kb on + strand, within Pf1N1B4_5520 at 275.424 kb on + strand, within Pf1N1B4_5520 at 275.485 kb on - strand, within Pf1N1B4_5520 at 275.670 kb on + strand, within Pf1N1B4_5520 at 275.673 kb on - strand, within Pf1N1B4_5520 at 275.719 kb on + strand at 275.735 kb on + strand, within Pf1N1B4_5520 at 275.852 kb on + strand at 275.899 kb on - strand at 275.899 kb on - strand at 275.899 kb on - strand at 275.997 kb on + strand at 276.104 kb on - strand at 276.112 kb on - strand at 276.112 kb on - strand at 276.475 kb on + strand at 276.480 kb on - strand at 276.591 kb on - strand at 276.717 kb on + strand at 276.832 kb on + strand at 276.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 273,225 + Pf1N1B4_5519 0.63 +0.0 273,245 + Pf1N1B4_5519 0.65 +0.2 273,406 - Pf1N1B4_5519 0.78 +0.2 273,679 - -0.3 273,791 - -0.5 273,861 - +0.6 274,735 - Pf1N1B4_5520 0.32 -0.7 274,830 + Pf1N1B4_5520 0.37 +0.2 274,831 - Pf1N1B4_5520 0.37 -0.5 274,831 - Pf1N1B4_5520 0.37 -1.4 274,897 - Pf1N1B4_5520 0.41 -0.4 274,900 + Pf1N1B4_5520 0.41 -1.2 274,900 + Pf1N1B4_5520 0.41 -1.3 274,904 + Pf1N1B4_5520 0.41 -1.4 274,904 + -0.4 275,023 + Pf1N1B4_5520 0.48 -0.8 275,024 - Pf1N1B4_5520 0.48 -0.5 275,108 - Pf1N1B4_5520 0.52 -0.8 275,108 - +0.2 275,179 - Pf1N1B4_5520 0.56 -2.1 275,220 + Pf1N1B4_5520 0.58 +1.2 275,373 + Pf1N1B4_5520 0.66 -0.6 275,424 + Pf1N1B4_5520 0.69 -1.6 275,485 - Pf1N1B4_5520 0.73 -2.0 275,670 + Pf1N1B4_5520 0.82 -2.6 275,673 - Pf1N1B4_5520 0.83 +0.7 275,719 + -0.8 275,735 + Pf1N1B4_5520 0.86 +1.5 275,852 + -0.4 275,899 - -2.0 275,899 - +1.8 275,899 - -1.0 275,997 + -0.8 276,104 - +0.2 276,112 - +2.2 276,112 - +0.2 276,475 + -0.4 276,480 - +2.4 276,591 - +1.2 276,717 + -0.1 276,832 + +1.2 276,833 - -1.8
Or see this region's nucleotide sequence