Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_543 and Pf1N1B4_544 are separated by 193 nucleotides Pf1N1B4_544 and Pf1N1B4_545 are separated by 105 nucleotides
Pf1N1B4_543 - ATP-dependent RNA helicase SrmB, at 639,950 to 641,296
_543
Pf1N1B4_544 - Small-conductance mechanosensitive channel, at 641,490 to 642,788
_544
Pf1N1B4_545 - MadN protein, at 642,894 to 643,760
_545
Position (kb) 641
642
643 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 641.317 kb on - strand at 641.335 kb on - strand at 641.372 kb on - strand at 641.373 kb on + strand at 641.373 kb on + strand at 641.440 kb on + strand at 641.440 kb on + strand at 641.440 kb on + strand at 641.441 kb on - strand at 641.441 kb on - strand at 641.441 kb on - strand at 641.602 kb on + strand at 641.603 kb on - strand at 641.786 kb on + strand, within Pf1N1B4_544 at 641.874 kb on + strand, within Pf1N1B4_544 at 642.378 kb on + strand, within Pf1N1B4_544 at 642.501 kb on + strand at 642.502 kb on - strand, within Pf1N1B4_544 at 642.591 kb on + strand, within Pf1N1B4_544 at 642.591 kb on + strand, within Pf1N1B4_544 at 642.592 kb on - strand, within Pf1N1B4_544 at 642.786 kb on + strand at 642.786 kb on + strand at 642.786 kb on + strand at 642.786 kb on + strand at 642.786 kb on + strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.787 kb on - strand at 642.907 kb on - strand at 642.907 kb on - strand at 642.932 kb on + strand at 643.099 kb on + strand, within Pf1N1B4_545 at 643.228 kb on + strand, within Pf1N1B4_545 at 643.228 kb on + strand, within Pf1N1B4_545 at 643.228 kb on + strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.229 kb on - strand, within Pf1N1B4_545 at 643.235 kb on - strand, within Pf1N1B4_545 at 643.291 kb on + strand, within Pf1N1B4_545 at 643.291 kb on + strand, within Pf1N1B4_545 at 643.292 kb on - strand, within Pf1N1B4_545 at 643.534 kb on + strand, within Pf1N1B4_545 at 643.547 kb on - strand, within Pf1N1B4_545 at 643.547 kb on - strand, within Pf1N1B4_545 at 643.547 kb on - strand, within Pf1N1B4_545 at 643.547 kb on - strand at 643.675 kb on + strand at 643.676 kb on - strand at 643.676 kb on - strand at 643.700 kb on - strand at 643.700 kb on - strand at 643.749 kb on + strand at 643.750 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 641,317 - +0.6 641,335 - +0.1 641,372 - +0.8 641,373 + -0.5 641,373 + -1.6 641,440 + +1.1 641,440 + -0.5 641,440 + +1.1 641,441 - +0.6 641,441 - -0.7 641,441 - +1.1 641,602 + -0.5 641,603 - +1.1 641,786 + Pf1N1B4_544 0.23 -1.2 641,874 + Pf1N1B4_544 0.30 -0.5 642,378 + Pf1N1B4_544 0.68 -1.2 642,501 + +1.1 642,502 - Pf1N1B4_544 0.78 -1.0 642,591 + Pf1N1B4_544 0.85 -0.6 642,591 + Pf1N1B4_544 0.85 -1.5 642,592 - Pf1N1B4_544 0.85 -1.3 642,786 + -2.7 642,786 + -1.7 642,786 + +0.1 642,786 + +0.3 642,786 + -1.1 642,787 - -1.7 642,787 - +0.1 642,787 - +1.1 642,787 - -1.7 642,787 - +0.4 642,787 - -1.9 642,787 - +0.1 642,787 - -0.7 642,907 - -0.5 642,907 - +0.1 642,932 + -1.9 643,099 + Pf1N1B4_545 0.24 +1.1 643,228 + Pf1N1B4_545 0.39 -0.1 643,228 + Pf1N1B4_545 0.39 -0.3 643,228 + Pf1N1B4_545 0.39 +2.1 643,229 - Pf1N1B4_545 0.39 +0.7 643,229 - Pf1N1B4_545 0.39 -2.6 643,229 - Pf1N1B4_545 0.39 -0.2 643,229 - Pf1N1B4_545 0.39 -1.9 643,229 - Pf1N1B4_545 0.39 +2.7 643,235 - Pf1N1B4_545 0.39 -3.1 643,291 + Pf1N1B4_545 0.46 -0.7 643,291 + Pf1N1B4_545 0.46 -0.2 643,292 - Pf1N1B4_545 0.46 +0.4 643,534 + Pf1N1B4_545 0.74 -1.2 643,547 - Pf1N1B4_545 0.75 +1.4 643,547 - Pf1N1B4_545 0.75 -0.1 643,547 - Pf1N1B4_545 0.75 +0.1 643,547 - +1.1 643,675 + +0.4 643,676 - -1.8 643,676 - +0.1 643,700 - +1.6 643,700 - +0.1 643,749 + +1.1 643,750 - -0.2
Or see this region's nucleotide sequence