Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5016 and Pf1N1B4_5017 are separated by 140 nucleotides Pf1N1B4_5017 and Pf1N1B4_5018 are separated by 177 nucleotides Pf1N1B4_5018 and Pf1N1B4_5019 are separated by 72 nucleotides Pf1N1B4_5019 and Pf1N1B4_5020 are separated by 212 nucleotides Pf1N1B4_5020 and Pf1N1B4_5021 are separated by 22 nucleotides
Pf1N1B4_5016 - hypothetical protein, at 5,567,715 to 5,567,903
_5016
Pf1N1B4_5017 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,568,044 to 5,568,325
_5017
Pf1N1B4_5018 - FIG00962345: hypothetical protein, at 5,568,503 to 5,568,733
_5018
Pf1N1B4_5019 - FIG00958773: hypothetical protein, at 5,568,806 to 5,569,162
_5019
Pf1N1B4_5020 - FIG00954920: hypothetical protein, at 5,569,375 to 5,569,677
_5020
Pf1N1B4_5021 - FIG00958580: hypothetical protein, at 5,569,700 to 5,569,876
_5021
Position (kb) 5568
5569 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5567.533 kb on - strand at 5567.552 kb on + strand at 5567.553 kb on - strand at 5567.581 kb on + strand at 5567.583 kb on + strand at 5567.583 kb on + strand at 5567.583 kb on + strand at 5567.584 kb on - strand at 5567.584 kb on - strand at 5567.584 kb on - strand at 5567.651 kb on - strand at 5567.712 kb on + strand at 5567.712 kb on + strand at 5567.712 kb on + strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.713 kb on - strand at 5567.955 kb on + strand at 5568.110 kb on + strand, within Pf1N1B4_5017 at 5568.110 kb on + strand, within Pf1N1B4_5017 at 5568.238 kb on + strand, within Pf1N1B4_5017 at 5568.291 kb on - strand, within Pf1N1B4_5017 at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.323 kb on + strand at 5568.324 kb on - strand at 5568.342 kb on - strand at 5568.361 kb on - strand at 5568.504 kb on + strand at 5568.504 kb on + strand at 5568.504 kb on + strand at 5568.505 kb on - strand at 5568.505 kb on - strand at 5568.508 kb on + strand at 5568.509 kb on - strand at 5568.509 kb on - strand at 5568.509 kb on - strand at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.691 kb on + strand, within Pf1N1B4_5018 at 5568.692 kb on - strand at 5568.692 kb on - strand, within Pf1N1B4_5018 at 5568.804 kb on + strand at 5568.805 kb on - strand at 5568.805 kb on - strand at 5568.839 kb on + strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.840 kb on - strand at 5568.924 kb on - strand, within Pf1N1B4_5019 at 5569.064 kb on + strand, within Pf1N1B4_5019 at 5569.229 kb on - strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.250 kb on - strand at 5569.699 kb on + strand at 5569.699 kb on + strand at 5569.700 kb on - strand at 5569.700 kb on - strand at 5569.725 kb on - strand at 5569.725 kb on - strand, within Pf1N1B4_5021
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 5,567,533 - -0.4 5,567,552 + -1.5 5,567,553 - +0.3 5,567,581 + -0.5 5,567,583 + -0.2 5,567,583 + +0.1 5,567,583 + -2.8 5,567,584 - -2.5 5,567,584 - -0.3 5,567,584 - -0.9 5,567,651 - -1.1 5,567,712 + +2.0 5,567,712 + +0.7 5,567,712 + -0.3 5,567,713 - -0.3 5,567,713 - -1.1 5,567,713 - -0.6 5,567,713 - -0.3 5,567,955 + +0.7 5,568,110 + Pf1N1B4_5017 0.23 +0.7 5,568,110 + Pf1N1B4_5017 0.23 -2.9 5,568,238 + Pf1N1B4_5017 0.69 -0.3 5,568,291 - Pf1N1B4_5017 0.88 +0.1 5,568,323 + +1.7 5,568,323 + -0.8 5,568,323 + -0.6 5,568,323 + -1.3 5,568,324 - +0.7 5,568,342 - -1.3 5,568,361 - -2.1 5,568,504 + -0.1 5,568,504 + -2.1 5,568,504 + -0.1 5,568,505 - -0.0 5,568,505 - -0.3 5,568,508 + +1.1 5,568,509 - +0.1 5,568,509 - -0.6 5,568,509 - +0.3 5,568,691 + Pf1N1B4_5018 0.81 -1.6 5,568,691 + Pf1N1B4_5018 0.81 -2.5 5,568,691 + Pf1N1B4_5018 0.81 -1.9 5,568,691 + Pf1N1B4_5018 0.81 +1.1 5,568,691 + Pf1N1B4_5018 0.81 -0.9 5,568,691 + Pf1N1B4_5018 0.81 -2.2 5,568,692 - -0.9 5,568,692 - Pf1N1B4_5018 0.82 +0.7 5,568,804 + +0.1 5,568,805 - -0.5 5,568,805 - +1.9 5,568,839 + +1.2 5,568,840 - -0.3 5,568,840 - +1.4 5,568,840 - -0.3 5,568,840 - +0.3 5,568,840 - -1.9 5,568,924 - Pf1N1B4_5019 0.33 +0.4 5,569,064 + Pf1N1B4_5019 0.72 +1.7 5,569,229 - +0.7 5,569,237 + -0.9 5,569,237 + +0.1 5,569,237 + +1.7 5,569,250 - +1.0 5,569,699 + +0.2 5,569,699 + +1.0 5,569,700 - +0.1 5,569,700 - -1.9 5,569,725 - -0.3 5,569,725 - Pf1N1B4_5021 0.14 -0.9
Or see this region's nucleotide sequence