Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4943 and Pf1N1B4_4944 are separated by 6 nucleotides Pf1N1B4_4944 and Pf1N1B4_4945 are separated by 114 nucleotides Pf1N1B4_4945 and Pf1N1B4_4946 are separated by 78 nucleotides Pf1N1B4_4946 and Pf1N1B4_4947 are separated by 306 nucleotides
Pf1N1B4_4943 - Oxidoreductase, short chain dehydrogenase/reductase family, at 5,479,228 to 5,480,019
_4943
Pf1N1B4_4944 - hypothetical protein, at 5,480,026 to 5,480,151
_4944
Pf1N1B4_4945 - hypothetical protein, at 5,480,266 to 5,480,439
_4945
Pf1N1B4_4946 - putative aminotransferase, at 5,480,518 to 5,481,672
_4946
Pf1N1B4_4947 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 5,481,979 to 5,485,044
_4947
Position (kb)
5480
5481
5482 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5479.985 kb on + strand at 5480.206 kb on + strand at 5480.206 kb on + strand at 5480.206 kb on + strand at 5480.224 kb on + strand at 5480.225 kb on - strand at 5480.282 kb on + strand at 5480.283 kb on - strand at 5480.332 kb on + strand, within Pf1N1B4_4945 at 5480.332 kb on + strand, within Pf1N1B4_4945 at 5480.333 kb on - strand, within Pf1N1B4_4945 at 5480.333 kb on - strand at 5480.469 kb on + strand at 5480.541 kb on + strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.542 kb on - strand at 5480.723 kb on + strand, within Pf1N1B4_4946 at 5480.978 kb on + strand, within Pf1N1B4_4946 at 5480.979 kb on - strand, within Pf1N1B4_4946 at 5480.979 kb on - strand, within Pf1N1B4_4946 at 5481.052 kb on + strand, within Pf1N1B4_4946 at 5481.053 kb on - strand, within Pf1N1B4_4946 at 5481.053 kb on - strand, within Pf1N1B4_4946 at 5481.245 kb on + strand at 5481.246 kb on - strand, within Pf1N1B4_4946 at 5481.446 kb on + strand, within Pf1N1B4_4946 at 5481.901 kb on + strand at 5482.165 kb on + strand at 5482.166 kb on - strand at 5482.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 5,479,985 + +0.1 5,480,206 + +0.0 5,480,206 + -1.9 5,480,206 + -1.0 5,480,224 + -0.3 5,480,225 - +0.7 5,480,282 + +0.3 5,480,283 - -0.3 5,480,332 + Pf1N1B4_4945 0.38 -1.9 5,480,332 + Pf1N1B4_4945 0.38 -0.5 5,480,333 - Pf1N1B4_4945 0.39 -0.9 5,480,333 - +1.7 5,480,469 + -1.0 5,480,541 + -0.9 5,480,542 - -0.3 5,480,542 - -0.3 5,480,542 - -0.3 5,480,542 - +3.2 5,480,542 - -0.6 5,480,723 + Pf1N1B4_4946 0.18 +0.1 5,480,978 + Pf1N1B4_4946 0.40 -2.6 5,480,979 - Pf1N1B4_4946 0.40 -1.6 5,480,979 - Pf1N1B4_4946 0.40 -0.9 5,481,052 + Pf1N1B4_4946 0.46 -1.6 5,481,053 - Pf1N1B4_4946 0.46 -1.6 5,481,053 - Pf1N1B4_4946 0.46 -2.0 5,481,245 + -0.3 5,481,246 - Pf1N1B4_4946 0.63 -0.9 5,481,446 + Pf1N1B4_4946 0.80 -0.1 5,481,901 + +0.7 5,482,165 + -0.0 5,482,166 - +0.5 5,482,166 - +0.3
Or see this region's nucleotide sequence