Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4800 and Pf1N1B4_4801 overlap by 4 nucleotides Pf1N1B4_4801 and Pf1N1B4_4802 overlap by 8 nucleotides Pf1N1B4_4802 and Pf1N1B4_4803 are separated by 402 nucleotides
Pf1N1B4_4800 - Immune-responsive protein 1, at 5,302,728 to 5,304,134
_4800
Pf1N1B4_4801 - Polysaccharide deacetylase, at 5,304,131 to 5,305,018
_4801
Pf1N1B4_4802 - polysaccharide deacetylase, at 5,305,011 to 5,305,859
_4802
Pf1N1B4_4803 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 5,306,262 to 5,307,047
_4803
Position (kb) 5305
5306 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5304.098 kb on - strand at 5304.141 kb on + strand at 5304.162 kb on + strand at 5304.246 kb on - strand, within Pf1N1B4_4801 at 5304.339 kb on + strand, within Pf1N1B4_4801 at 5304.339 kb on + strand, within Pf1N1B4_4801 at 5304.394 kb on + strand, within Pf1N1B4_4801 at 5304.403 kb on - strand, within Pf1N1B4_4801 at 5304.715 kb on - strand, within Pf1N1B4_4801 at 5304.957 kb on + strand at 5305.229 kb on - strand, within Pf1N1B4_4802 at 5305.291 kb on + strand, within Pf1N1B4_4802 at 5305.291 kb on + strand, within Pf1N1B4_4802 at 5305.361 kb on - strand at 5305.394 kb on - strand, within Pf1N1B4_4802 at 5305.735 kb on + strand at 5305.874 kb on - strand at 5305.877 kb on + strand at 5305.877 kb on + strand at 5305.878 kb on - strand at 5305.878 kb on - strand at 5305.879 kb on + strand at 5305.880 kb on - strand at 5305.883 kb on + strand at 5306.025 kb on - strand at 5306.088 kb on - strand at 5306.208 kb on + strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.263 kb on + strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.455 kb on + strand, within Pf1N1B4_4803 at 5306.456 kb on - strand, within Pf1N1B4_4803 at 5306.456 kb on - strand, within Pf1N1B4_4803 at 5306.456 kb on - strand, within Pf1N1B4_4803 at 5306.744 kb on - strand, within Pf1N1B4_4803
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 5,304,098 - +2.2 5,304,141 + +1.0 5,304,162 + +1.2 5,304,246 - Pf1N1B4_4801 0.13 +0.2 5,304,339 + Pf1N1B4_4801 0.23 +1.2 5,304,339 + Pf1N1B4_4801 0.23 +0.2 5,304,394 + Pf1N1B4_4801 0.30 +0.1 5,304,403 - Pf1N1B4_4801 0.31 +1.0 5,304,715 - Pf1N1B4_4801 0.66 +0.5 5,304,957 + +0.8 5,305,229 - Pf1N1B4_4802 0.26 -1.4 5,305,291 + Pf1N1B4_4802 0.33 -0.2 5,305,291 + Pf1N1B4_4802 0.33 -1.3 5,305,361 - +0.7 5,305,394 - Pf1N1B4_4802 0.45 -0.9 5,305,735 + -0.3 5,305,874 - -0.5 5,305,877 + -1.2 5,305,877 + -0.8 5,305,878 - -0.9 5,305,878 - -2.5 5,305,879 + -1.2 5,305,880 - -2.9 5,305,883 + -1.3 5,306,025 - -2.6 5,306,088 - +1.7 5,306,208 + +0.3 5,306,209 - +0.7 5,306,209 - +1.2 5,306,209 - +1.0 5,306,209 - +0.2 5,306,209 - -2.2 5,306,209 - -0.8 5,306,263 + +0.7 5,306,264 - +1.3 5,306,264 - +0.2 5,306,264 - +0.4 5,306,264 - -0.3 5,306,264 - +0.2 5,306,264 - +0.5 5,306,455 + Pf1N1B4_4803 0.25 -0.3 5,306,456 - Pf1N1B4_4803 0.25 -0.8 5,306,456 - Pf1N1B4_4803 0.25 -0.3 5,306,456 - Pf1N1B4_4803 0.25 -1.3 5,306,744 - Pf1N1B4_4803 0.61 -0.2
Or see this region's nucleotide sequence