Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4237 and Pf1N1B4_4238 are separated by 260 nucleotides Pf1N1B4_4238 and Pf1N1B4_4239 are separated by 54 nucleotides Pf1N1B4_4239 and Pf1N1B4_4240 are separated by 137 nucleotides
Pf1N1B4_4237 - COG1180: Radical SAM, Pyruvate-formate lyase-activating enzyme like, at 4,696,065 to 4,697,528
_4237
Pf1N1B4_4238 - hypothetical protein, at 4,697,789 to 4,698,370
_4238
Pf1N1B4_4239 - hypothetical protein, at 4,698,425 to 4,698,976
_4239
Pf1N1B4_4240 - hypothetical protein, at 4,699,114 to 4,699,263
_4240
Position (kb) 4697
4698
4699 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4696.804 kb on + strand, within Pf1N1B4_4237 at 4696.805 kb on - strand at 4696.814 kb on + strand, within Pf1N1B4_4237 at 4696.815 kb on - strand, within Pf1N1B4_4237 at 4697.217 kb on - strand, within Pf1N1B4_4237 at 4697.232 kb on + strand, within Pf1N1B4_4237 at 4697.232 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.243 kb on - strand, within Pf1N1B4_4237 at 4697.243 kb on - strand, within Pf1N1B4_4237 at 4697.243 kb on - strand, within Pf1N1B4_4237 at 4697.296 kb on + strand, within Pf1N1B4_4237 at 4697.476 kb on + strand at 4697.613 kb on + strand at 4698.019 kb on - strand, within Pf1N1B4_4238 at 4698.043 kb on - strand, within Pf1N1B4_4238 at 4698.057 kb on + strand, within Pf1N1B4_4238 at 4698.058 kb on - strand at 4698.196 kb on - strand, within Pf1N1B4_4238 at 4698.196 kb on - strand, within Pf1N1B4_4238 at 4698.306 kb on - strand, within Pf1N1B4_4238 at 4698.376 kb on + strand at 4698.384 kb on + strand at 4698.384 kb on + strand at 4698.384 kb on + strand at 4698.385 kb on - strand at 4698.726 kb on + strand, within Pf1N1B4_4239 at 4698.727 kb on - strand, within Pf1N1B4_4239 at 4698.732 kb on + strand at 4698.733 kb on - strand, within Pf1N1B4_4239 at 4698.733 kb on - strand, within Pf1N1B4_4239 at 4698.873 kb on + strand, within Pf1N1B4_4239 at 4698.873 kb on + strand, within Pf1N1B4_4239 at 4698.873 kb on + strand at 4698.874 kb on - strand, within Pf1N1B4_4239 at 4698.880 kb on - strand, within Pf1N1B4_4239 at 4699.076 kb on + strand at 4699.109 kb on - strand at 4699.115 kb on + strand at 4699.165 kb on - strand, within Pf1N1B4_4240 at 4699.168 kb on + strand, within Pf1N1B4_4240 at 4699.169 kb on - strand, within Pf1N1B4_4240 at 4699.281 kb on + strand at 4699.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 4,696,804 + Pf1N1B4_4237 0.50 -0.7 4,696,805 - -1.1 4,696,814 + Pf1N1B4_4237 0.51 +0.2 4,696,815 - Pf1N1B4_4237 0.51 -0.2 4,697,217 - Pf1N1B4_4237 0.79 -1.9 4,697,232 + Pf1N1B4_4237 0.80 +1.0 4,697,232 + Pf1N1B4_4237 0.80 -0.4 4,697,242 + Pf1N1B4_4237 0.80 -1.1 4,697,242 + Pf1N1B4_4237 0.80 +1.2 4,697,242 + Pf1N1B4_4237 0.80 -0.7 4,697,242 + Pf1N1B4_4237 0.80 +0.3 4,697,242 + Pf1N1B4_4237 0.80 +0.1 4,697,243 - Pf1N1B4_4237 0.80 +2.1 4,697,243 - Pf1N1B4_4237 0.80 -0.9 4,697,243 - Pf1N1B4_4237 0.80 +0.4 4,697,296 + Pf1N1B4_4237 0.84 +1.5 4,697,476 + -2.3 4,697,613 + -1.4 4,698,019 - Pf1N1B4_4238 0.40 -1.0 4,698,043 - Pf1N1B4_4238 0.44 -1.4 4,698,057 + Pf1N1B4_4238 0.46 -0.7 4,698,058 - +0.9 4,698,196 - Pf1N1B4_4238 0.70 -1.4 4,698,196 - Pf1N1B4_4238 0.70 -1.7 4,698,306 - Pf1N1B4_4238 0.89 -0.6 4,698,376 + +1.4 4,698,384 + +0.2 4,698,384 + -0.1 4,698,384 + -1.1 4,698,385 - -1.9 4,698,726 + Pf1N1B4_4239 0.55 -0.5 4,698,727 - Pf1N1B4_4239 0.55 +0.9 4,698,732 + -0.1 4,698,733 - Pf1N1B4_4239 0.56 -0.7 4,698,733 - Pf1N1B4_4239 0.56 +0.6 4,698,873 + Pf1N1B4_4239 0.81 +0.3 4,698,873 + Pf1N1B4_4239 0.81 +1.3 4,698,873 + -0.1 4,698,874 - Pf1N1B4_4239 0.81 +0.9 4,698,880 - Pf1N1B4_4239 0.82 -1.3 4,699,076 + +0.7 4,699,109 - -0.7 4,699,115 + +1.5 4,699,165 - Pf1N1B4_4240 0.34 +0.9 4,699,168 + Pf1N1B4_4240 0.36 +1.4 4,699,169 - Pf1N1B4_4240 0.37 +0.0 4,699,281 + -0.1 4,699,282 - -2.1
Or see this region's nucleotide sequence