Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4212 and Pf1N1B4_4213 are separated by 38 nucleotides Pf1N1B4_4213 and Pf1N1B4_4214 are separated by 106 nucleotides
Pf1N1B4_4212 - Gfa-like protein, at 4,666,462 to 4,666,902
_4212
Pf1N1B4_4213 - Potassium channel protein, at 4,666,941 to 4,667,816
_4213
Pf1N1B4_4214 - Arginine/ornithine antiporter ArcD, at 4,667,923 to 4,669,389
_4214
Position (kb)
4666
4667
4668 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4666.349 kb on + strand at 4666.437 kb on + strand at 4666.462 kb on - strand at 4666.492 kb on + strand at 4666.492 kb on + strand at 4666.492 kb on + strand at 4666.493 kb on - strand at 4666.503 kb on + strand at 4666.525 kb on + strand at 4666.730 kb on + strand, within Pf1N1B4_4212 at 4666.730 kb on + strand, within Pf1N1B4_4212 at 4666.730 kb on + strand, within Pf1N1B4_4212 at 4666.730 kb on + strand, within Pf1N1B4_4212 at 4666.733 kb on + strand, within Pf1N1B4_4212 at 4666.893 kb on + strand at 4667.049 kb on - strand, within Pf1N1B4_4213 at 4667.049 kb on - strand, within Pf1N1B4_4213 at 4667.094 kb on + strand, within Pf1N1B4_4213 at 4667.094 kb on + strand, within Pf1N1B4_4213 at 4667.188 kb on - strand, within Pf1N1B4_4213 at 4667.188 kb on - strand, within Pf1N1B4_4213 at 4667.194 kb on - strand, within Pf1N1B4_4213 at 4667.197 kb on + strand, within Pf1N1B4_4213 at 4667.198 kb on - strand, within Pf1N1B4_4213 at 4667.198 kb on - strand, within Pf1N1B4_4213 at 4667.249 kb on + strand, within Pf1N1B4_4213 at 4667.249 kb on + strand, within Pf1N1B4_4213 at 4667.251 kb on + strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.261 kb on + strand, within Pf1N1B4_4213 at 4667.433 kb on + strand, within Pf1N1B4_4213 at 4667.434 kb on - strand, within Pf1N1B4_4213 at 4667.436 kb on - strand, within Pf1N1B4_4213 at 4667.475 kb on + strand, within Pf1N1B4_4213 at 4667.499 kb on - strand at 4667.653 kb on - strand, within Pf1N1B4_4213 at 4667.653 kb on - strand, within Pf1N1B4_4213 at 4667.696 kb on + strand, within Pf1N1B4_4213 at 4667.696 kb on + strand, within Pf1N1B4_4213 at 4667.757 kb on + strand at 4667.757 kb on + strand at 4667.758 kb on - strand at 4667.945 kb on + strand at 4667.945 kb on + strand at 4667.974 kb on + strand at 4667.974 kb on + strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4668.072 kb on - strand, within Pf1N1B4_4214 at 4668.113 kb on + strand, within Pf1N1B4_4214 at 4668.124 kb on + strand, within Pf1N1B4_4214 at 4668.132 kb on - strand at 4668.191 kb on + strand, within Pf1N1B4_4214 at 4668.192 kb on - strand at 4668.273 kb on - strand, within Pf1N1B4_4214 at 4668.273 kb on - strand, within Pf1N1B4_4214 at 4668.273 kb on - strand, within Pf1N1B4_4214 at 4668.283 kb on + strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.288 kb on - strand, within Pf1N1B4_4214 at 4668.288 kb on - strand, within Pf1N1B4_4214 at 4668.288 kb on - strand, within Pf1N1B4_4214 at 4668.313 kb on - strand, within Pf1N1B4_4214 at 4668.350 kb on - strand, within Pf1N1B4_4214 at 4668.620 kb on - strand, within Pf1N1B4_4214 at 4668.620 kb on - strand, within Pf1N1B4_4214 at 4668.718 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 4,666,349 + -0.1 4,666,437 + +0.9 4,666,462 - +0.9 4,666,492 + -0.3 4,666,492 + -0.1 4,666,492 + +1.1 4,666,493 - -0.1 4,666,503 + +2.9 4,666,525 + +0.9 4,666,730 + Pf1N1B4_4212 0.61 -1.4 4,666,730 + Pf1N1B4_4212 0.61 -2.6 4,666,730 + Pf1N1B4_4212 0.61 +1.9 4,666,730 + Pf1N1B4_4212 0.61 -1.3 4,666,733 + Pf1N1B4_4212 0.61 +0.3 4,666,893 + -1.7 4,667,049 - Pf1N1B4_4213 0.12 -1.9 4,667,049 - Pf1N1B4_4213 0.12 -1.7 4,667,094 + Pf1N1B4_4213 0.17 +1.9 4,667,094 + Pf1N1B4_4213 0.17 -1.1 4,667,188 - Pf1N1B4_4213 0.28 +0.4 4,667,188 - Pf1N1B4_4213 0.28 -1.1 4,667,194 - Pf1N1B4_4213 0.29 +1.9 4,667,197 + Pf1N1B4_4213 0.29 -1.1 4,667,198 - Pf1N1B4_4213 0.29 -1.4 4,667,198 - Pf1N1B4_4213 0.29 -2.1 4,667,249 + Pf1N1B4_4213 0.35 +1.7 4,667,249 + Pf1N1B4_4213 0.35 -0.4 4,667,251 + Pf1N1B4_4213 0.35 -0.7 4,667,252 - Pf1N1B4_4213 0.36 +0.7 4,667,252 - Pf1N1B4_4213 0.36 +0.9 4,667,252 - Pf1N1B4_4213 0.36 +1.2 4,667,252 - Pf1N1B4_4213 0.36 -1.3 4,667,252 - Pf1N1B4_4213 0.36 -1.4 4,667,261 + Pf1N1B4_4213 0.37 -0.4 4,667,433 + Pf1N1B4_4213 0.56 -0.1 4,667,434 - Pf1N1B4_4213 0.56 -1.1 4,667,436 - Pf1N1B4_4213 0.57 -1.3 4,667,475 + Pf1N1B4_4213 0.61 -0.2 4,667,499 - -0.1 4,667,653 - Pf1N1B4_4213 0.81 +0.1 4,667,653 - Pf1N1B4_4213 0.81 -0.4 4,667,696 + Pf1N1B4_4213 0.86 -1.4 4,667,696 + Pf1N1B4_4213 0.86 -1.5 4,667,757 + -0.3 4,667,757 + +0.9 4,667,758 - -1.1 4,667,945 + -1.3 4,667,945 + -0.1 4,667,974 + -0.7 4,667,974 + +0.1 4,667,975 - -0.1 4,667,975 - -1.4 4,667,975 - +0.1 4,667,975 - +0.9 4,667,975 - +2.5 4,667,975 - +0.9 4,667,975 - +0.6 4,667,975 - -0.1 4,667,975 - -0.3 4,668,072 - Pf1N1B4_4214 0.10 -2.7 4,668,113 + Pf1N1B4_4214 0.13 -0.2 4,668,124 + Pf1N1B4_4214 0.14 -0.1 4,668,132 - +0.9 4,668,191 + Pf1N1B4_4214 0.18 +0.7 4,668,192 - +0.9 4,668,273 - Pf1N1B4_4214 0.24 -1.7 4,668,273 - Pf1N1B4_4214 0.24 -0.7 4,668,273 - Pf1N1B4_4214 0.24 -0.5 4,668,283 + Pf1N1B4_4214 0.25 +0.1 4,668,284 - Pf1N1B4_4214 0.25 +0.9 4,668,284 - Pf1N1B4_4214 0.25 -0.4 4,668,284 - Pf1N1B4_4214 0.25 -1.4 4,668,284 - Pf1N1B4_4214 0.25 +1.2 4,668,288 - Pf1N1B4_4214 0.25 -0.9 4,668,288 - Pf1N1B4_4214 0.25 +0.4 4,668,288 - Pf1N1B4_4214 0.25 -1.7 4,668,313 - Pf1N1B4_4214 0.27 +0.1 4,668,350 - Pf1N1B4_4214 0.29 +0.2 4,668,620 - Pf1N1B4_4214 0.48 +0.3 4,668,620 - Pf1N1B4_4214 0.48 +2.2 4,668,718 - -1.1
Or see this region's nucleotide sequence