Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_402 and Pf1N1B4_403 are separated by 462 nucleotides Pf1N1B4_403 and Pf1N1B4_404 are separated by 112 nucleotides Pf1N1B4_404 and Pf1N1B4_405 are separated by 135 nucleotides
Pf1N1B4_402 - metal-dependent phosphohydrolase, at 471,295 to 471,951
_402
Pf1N1B4_403 - FIG00965925: hypothetical protein, at 472,414 to 472,668
_403
Pf1N1B4_404 - ParE toxin protein, at 472,781 to 473,161
_404
Pf1N1B4_405 - Carbohydrate kinase, PfkB family, at 473,297 to 474,226
_405
Position (kb) 472
473 Strain fitness (log2 ratio) -2
-1
0
1
2 at 471.564 kb on - strand, within Pf1N1B4_402 at 471.591 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.755 kb on - strand at 471.755 kb on - strand, within Pf1N1B4_402 at 471.755 kb on - strand, within Pf1N1B4_402 at 471.755 kb on - strand, within Pf1N1B4_402 at 471.790 kb on + strand, within Pf1N1B4_402 at 471.949 kb on + strand at 471.949 kb on + strand at 472.025 kb on + strand at 472.260 kb on + strand at 472.260 kb on + strand at 472.260 kb on + strand at 472.260 kb on + strand at 472.261 kb on - strand at 472.261 kb on - strand at 472.385 kb on + strand at 472.385 kb on + strand at 472.385 kb on + strand at 472.385 kb on + strand at 472.386 kb on - strand at 472.386 kb on - strand at 472.410 kb on - strand at 472.425 kb on + strand at 472.460 kb on + strand, within Pf1N1B4_403 at 472.461 kb on - strand, within Pf1N1B4_403 at 472.461 kb on - strand, within Pf1N1B4_403 at 472.461 kb on - strand, within Pf1N1B4_403 at 472.667 kb on - strand at 472.674 kb on + strand at 472.762 kb on + strand at 472.805 kb on + strand at 472.805 kb on + strand at 472.805 kb on + strand at 472.806 kb on - strand at 472.806 kb on - strand at 472.806 kb on - strand at 472.850 kb on + strand, within Pf1N1B4_404 at 472.850 kb on + strand, within Pf1N1B4_404 at 472.850 kb on + strand, within Pf1N1B4_404 at 472.851 kb on - strand, within Pf1N1B4_404 at 473.256 kb on - strand at 473.256 kb on - strand at 473.256 kb on - strand at 473.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 471,564 - Pf1N1B4_402 0.41 -2.0 471,591 + Pf1N1B4_402 0.45 +2.0 471,724 + +0.0 471,724 + Pf1N1B4_402 0.65 +0.3 471,724 + Pf1N1B4_402 0.65 +1.6 471,724 + Pf1N1B4_402 0.65 +1.0 471,724 + Pf1N1B4_402 0.65 +1.0 471,725 - Pf1N1B4_402 0.65 +0.4 471,725 - Pf1N1B4_402 0.65 -0.6 471,725 - Pf1N1B4_402 0.65 -0.5 471,725 - Pf1N1B4_402 0.65 +0.0 471,725 - Pf1N1B4_402 0.65 +0.4 471,754 + Pf1N1B4_402 0.70 -0.6 471,754 + Pf1N1B4_402 0.70 -1.6 471,754 + Pf1N1B4_402 0.70 -1.3 471,755 - +0.0 471,755 - Pf1N1B4_402 0.70 -1.0 471,755 - Pf1N1B4_402 0.70 +0.0 471,755 - Pf1N1B4_402 0.70 -0.4 471,790 + Pf1N1B4_402 0.75 +0.0 471,949 + -1.3 471,949 + +0.0 472,025 + +2.0 472,260 + -0.7 472,260 + +0.0 472,260 + +0.7 472,260 + -0.3 472,261 - -0.2 472,261 - +0.6 472,385 + -0.6 472,385 + -1.8 472,385 + +0.2 472,385 + -1.6 472,386 - -1.8 472,386 - +0.9 472,410 - -1.3 472,425 + -0.1 472,460 + Pf1N1B4_403 0.18 -1.8 472,461 - Pf1N1B4_403 0.18 -2.4 472,461 - Pf1N1B4_403 0.18 -1.6 472,461 - Pf1N1B4_403 0.18 -0.1 472,667 - +0.2 472,674 + -1.8 472,762 + -0.3 472,805 + +0.4 472,805 + +1.0 472,805 + +1.1 472,806 - -1.3 472,806 - -1.6 472,806 - -2.6 472,850 + Pf1N1B4_404 0.18 +0.2 472,850 + Pf1N1B4_404 0.18 -2.3 472,850 + Pf1N1B4_404 0.18 -0.2 472,851 - Pf1N1B4_404 0.18 -1.3 473,256 - -0.4 473,256 - -1.0 473,256 - -0.3 473,312 - +1.6
Or see this region's nucleotide sequence