Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3956 and Pf1N1B4_3957 are separated by 170 nucleotides Pf1N1B4_3957 and Pf1N1B4_3958 are separated by 190 nucleotides
Pf1N1B4_3956 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 4,395,335 to 4,396,030
_3956
Pf1N1B4_3957 - cytochrome b(561), at 4,396,201 to 4,396,749
_3957
Pf1N1B4_3958 - Phosphonoacetaldehyde hydrolase (EC 3.11.1.1), at 4,396,940 to 4,397,767
_3958
Position (kb)
4396
4397 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4395.523 kb on - strand, within Pf1N1B4_3956 at 4395.523 kb on - strand, within Pf1N1B4_3956 at 4395.627 kb on - strand, within Pf1N1B4_3956 at 4395.707 kb on + strand, within Pf1N1B4_3956 at 4395.708 kb on - strand, within Pf1N1B4_3956 at 4395.759 kb on + strand at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.759 kb on + strand, within Pf1N1B4_3956 at 4395.760 kb on - strand, within Pf1N1B4_3956 at 4395.760 kb on - strand, within Pf1N1B4_3956 at 4395.760 kb on - strand at 4395.794 kb on + strand, within Pf1N1B4_3956 at 4395.902 kb on + strand, within Pf1N1B4_3956 at 4396.043 kb on + strand at 4396.043 kb on + strand at 4396.250 kb on + strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.251 kb on - strand at 4396.260 kb on - strand at 4396.260 kb on - strand, within Pf1N1B4_3957 at 4396.260 kb on - strand, within Pf1N1B4_3957 at 4396.385 kb on + strand, within Pf1N1B4_3957 at 4396.386 kb on - strand, within Pf1N1B4_3957 at 4396.386 kb on - strand, within Pf1N1B4_3957 at 4396.455 kb on + strand, within Pf1N1B4_3957 at 4396.456 kb on - strand, within Pf1N1B4_3957 at 4396.456 kb on - strand, within Pf1N1B4_3957 at 4396.458 kb on - strand, within Pf1N1B4_3957 at 4396.458 kb on - strand at 4396.664 kb on + strand, within Pf1N1B4_3957 at 4396.665 kb on - strand, within Pf1N1B4_3957 at 4396.665 kb on - strand, within Pf1N1B4_3957 at 4396.682 kb on - strand, within Pf1N1B4_3957 at 4396.711 kb on + strand at 4396.711 kb on + strand at 4396.722 kb on - strand at 4396.749 kb on + strand at 4396.749 kb on + strand at 4396.813 kb on + strand at 4396.813 kb on + strand at 4396.813 kb on + strand at 4396.813 kb on + strand at 4396.813 kb on + strand at 4396.872 kb on - strand at 4396.872 kb on - strand at 4397.028 kb on + strand at 4397.028 kb on + strand, within Pf1N1B4_3958 at 4397.028 kb on + strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.029 kb on - strand, within Pf1N1B4_3958 at 4397.107 kb on - strand, within Pf1N1B4_3958 at 4397.116 kb on - strand, within Pf1N1B4_3958 at 4397.326 kb on - strand, within Pf1N1B4_3958 at 4397.499 kb on + strand, within Pf1N1B4_3958 at 4397.499 kb on + strand, within Pf1N1B4_3958 at 4397.500 kb on - strand, within Pf1N1B4_3958 at 4397.500 kb on - strand, within Pf1N1B4_3958
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 4,395,523 - Pf1N1B4_3956 0.27 -0.3 4,395,523 - Pf1N1B4_3956 0.27 +0.7 4,395,627 - Pf1N1B4_3956 0.42 -0.7 4,395,707 + Pf1N1B4_3956 0.53 -1.4 4,395,708 - Pf1N1B4_3956 0.54 -0.7 4,395,759 + -0.1 4,395,759 + Pf1N1B4_3956 0.61 -2.1 4,395,759 + Pf1N1B4_3956 0.61 +0.6 4,395,759 + Pf1N1B4_3956 0.61 -0.7 4,395,759 + Pf1N1B4_3956 0.61 -1.4 4,395,759 + Pf1N1B4_3956 0.61 +1.9 4,395,759 + Pf1N1B4_3956 0.61 +0.9 4,395,760 - Pf1N1B4_3956 0.61 -1.1 4,395,760 - Pf1N1B4_3956 0.61 +1.3 4,395,760 - -1.1 4,395,794 + Pf1N1B4_3956 0.66 -1.4 4,395,902 + Pf1N1B4_3956 0.81 -1.1 4,396,043 + +0.9 4,396,043 + -1.7 4,396,250 + -0.5 4,396,251 - -1.1 4,396,251 - -0.7 4,396,251 - -1.3 4,396,251 - +0.6 4,396,251 - +0.2 4,396,251 - -0.1 4,396,260 - +0.3 4,396,260 - Pf1N1B4_3957 0.11 -0.7 4,396,260 - Pf1N1B4_3957 0.11 -0.1 4,396,385 + Pf1N1B4_3957 0.34 +0.7 4,396,386 - Pf1N1B4_3957 0.34 -1.4 4,396,386 - Pf1N1B4_3957 0.34 +0.9 4,396,455 + Pf1N1B4_3957 0.46 +2.7 4,396,456 - Pf1N1B4_3957 0.46 -0.8 4,396,456 - Pf1N1B4_3957 0.46 +1.5 4,396,458 - Pf1N1B4_3957 0.47 -1.1 4,396,458 - +2.5 4,396,664 + Pf1N1B4_3957 0.84 -2.7 4,396,665 - Pf1N1B4_3957 0.85 -0.1 4,396,665 - Pf1N1B4_3957 0.85 +0.5 4,396,682 - Pf1N1B4_3957 0.88 +1.0 4,396,711 + -1.3 4,396,711 + +1.0 4,396,722 - -2.4 4,396,749 + +1.2 4,396,749 + -2.1 4,396,813 + +0.3 4,396,813 + -0.3 4,396,813 + -0.1 4,396,813 + +0.3 4,396,813 + -0.7 4,396,872 - -0.7 4,396,872 - -1.1 4,397,028 + +1.5 4,397,028 + Pf1N1B4_3958 0.11 -1.7 4,397,028 + Pf1N1B4_3958 0.11 +1.1 4,397,029 - Pf1N1B4_3958 0.11 +0.3 4,397,029 - Pf1N1B4_3958 0.11 -0.1 4,397,029 - Pf1N1B4_3958 0.11 -1.7 4,397,029 - Pf1N1B4_3958 0.11 -1.4 4,397,029 - Pf1N1B4_3958 0.11 +0.1 4,397,029 - Pf1N1B4_3958 0.11 +0.1 4,397,029 - Pf1N1B4_3958 0.11 +1.7 4,397,029 - Pf1N1B4_3958 0.11 -0.7 4,397,029 - Pf1N1B4_3958 0.11 -1.1 4,397,107 - Pf1N1B4_3958 0.20 -1.9 4,397,116 - Pf1N1B4_3958 0.21 +0.2 4,397,326 - Pf1N1B4_3958 0.47 -0.8 4,397,499 + Pf1N1B4_3958 0.68 +0.7 4,397,499 + Pf1N1B4_3958 0.68 -0.1 4,397,500 - Pf1N1B4_3958 0.68 -0.1 4,397,500 - Pf1N1B4_3958 0.68 -3.7
Or see this region's nucleotide sequence