Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3410 and Pf1N1B4_3411 are separated by 145 nucleotides Pf1N1B4_3411 and Pf1N1B4_3412 overlap by 4 nucleotides Pf1N1B4_3412 and Pf1N1B4_3413 are separated by 78 nucleotides Pf1N1B4_3413 and Pf1N1B4_3414 are separated by 6 nucleotides
Pf1N1B4_3410 - FIG00964654: hypothetical protein, at 3,802,196 to 3,802,522
_3410
Pf1N1B4_3411 - FIG00959431: hypothetical protein, at 3,802,668 to 3,802,991
_3411
Pf1N1B4_3412 - CTP synthase (EC 6.3.4.2), at 3,802,988 to 3,803,695
_3412
Pf1N1B4_3413 - Transcriptional regulator, LysR family, at 3,803,774 to 3,804,652
_3413
Pf1N1B4_3414 - PROBABLE MEMBRANE PROTEIN, at 3,804,659 to 3,804,907
_3414
Position (kb)
3802
3803
3804 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3801.998 kb on + strand at 3801.999 kb on - strand at 3802.005 kb on + strand at 3802.006 kb on - strand at 3802.044 kb on + strand at 3802.044 kb on + strand at 3802.045 kb on - strand at 3802.045 kb on - strand at 3802.045 kb on - strand at 3802.094 kb on + strand at 3802.094 kb on + strand at 3802.192 kb on + strand at 3802.277 kb on + strand, within Pf1N1B4_3410 at 3802.277 kb on + strand, within Pf1N1B4_3410 at 3802.277 kb on + strand, within Pf1N1B4_3410 at 3802.277 kb on + strand, within Pf1N1B4_3410 at 3802.278 kb on - strand, within Pf1N1B4_3410 at 3802.291 kb on + strand, within Pf1N1B4_3410 at 3802.463 kb on + strand, within Pf1N1B4_3410 at 3802.463 kb on + strand, within Pf1N1B4_3410 at 3802.463 kb on + strand, within Pf1N1B4_3410 at 3802.463 kb on + strand, within Pf1N1B4_3410 at 3802.464 kb on - strand at 3802.464 kb on - strand, within Pf1N1B4_3410 at 3802.464 kb on - strand, within Pf1N1B4_3410 at 3802.464 kb on - strand, within Pf1N1B4_3410 at 3802.464 kb on - strand, within Pf1N1B4_3410 at 3802.464 kb on - strand, within Pf1N1B4_3410 at 3802.464 kb on - strand, within Pf1N1B4_3410 at 3802.464 kb on - strand, within Pf1N1B4_3410 at 3802.464 kb on - strand, within Pf1N1B4_3410 at 3802.464 kb on - strand, within Pf1N1B4_3410 at 3802.465 kb on + strand, within Pf1N1B4_3410 at 3802.465 kb on + strand, within Pf1N1B4_3410 at 3802.466 kb on - strand, within Pf1N1B4_3410 at 3802.669 kb on + strand at 3802.669 kb on + strand at 3802.670 kb on - strand at 3802.684 kb on + strand at 3802.684 kb on + strand at 3802.685 kb on - strand at 3802.685 kb on - strand at 3802.713 kb on - strand, within Pf1N1B4_3411 at 3802.726 kb on + strand, within Pf1N1B4_3411 at 3802.811 kb on - strand, within Pf1N1B4_3411 at 3802.820 kb on + strand, within Pf1N1B4_3411 at 3802.820 kb on + strand, within Pf1N1B4_3411 at 3802.820 kb on + strand, within Pf1N1B4_3411 at 3802.946 kb on + strand, within Pf1N1B4_3411 at 3802.947 kb on - strand, within Pf1N1B4_3411 at 3802.947 kb on - strand, within Pf1N1B4_3411 at 3802.960 kb on + strand at 3802.964 kb on - strand at 3802.964 kb on - strand at 3803.051 kb on - strand at 3803.105 kb on + strand, within Pf1N1B4_3412 at 3803.105 kb on - strand, within Pf1N1B4_3412 at 3803.168 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.808 kb on - strand at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.439 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 3,801,998 + -0.6 3,801,999 - +0.3 3,802,005 + -2.8 3,802,006 - +0.1 3,802,044 + -1.3 3,802,044 + +1.2 3,802,045 - -1.5 3,802,045 - -1.5 3,802,045 - -0.5 3,802,094 + +0.1 3,802,094 + +0.1 3,802,192 + +0.3 3,802,277 + Pf1N1B4_3410 0.25 +1.1 3,802,277 + Pf1N1B4_3410 0.25 -1.9 3,802,277 + Pf1N1B4_3410 0.25 -1.5 3,802,277 + Pf1N1B4_3410 0.25 -0.9 3,802,278 - Pf1N1B4_3410 0.25 -1.8 3,802,291 + Pf1N1B4_3410 0.29 +2.1 3,802,463 + Pf1N1B4_3410 0.82 +0.2 3,802,463 + Pf1N1B4_3410 0.82 -0.1 3,802,463 + Pf1N1B4_3410 0.82 -0.5 3,802,463 + Pf1N1B4_3410 0.82 -0.3 3,802,464 - -0.5 3,802,464 - Pf1N1B4_3410 0.82 -1.3 3,802,464 - Pf1N1B4_3410 0.82 -0.6 3,802,464 - Pf1N1B4_3410 0.82 +1.2 3,802,464 - Pf1N1B4_3410 0.82 -2.4 3,802,464 - Pf1N1B4_3410 0.82 -2.4 3,802,464 - Pf1N1B4_3410 0.82 -0.5 3,802,464 - Pf1N1B4_3410 0.82 -0.5 3,802,464 - Pf1N1B4_3410 0.82 +0.3 3,802,464 - Pf1N1B4_3410 0.82 -1.3 3,802,465 + Pf1N1B4_3410 0.82 -0.3 3,802,465 + Pf1N1B4_3410 0.82 +1.3 3,802,466 - Pf1N1B4_3410 0.83 -1.8 3,802,669 + -1.0 3,802,669 + -0.4 3,802,670 - -0.9 3,802,684 + +0.3 3,802,684 + -1.3 3,802,685 - +0.3 3,802,685 - -3.0 3,802,713 - Pf1N1B4_3411 0.14 -0.3 3,802,726 + Pf1N1B4_3411 0.18 -1.5 3,802,811 - Pf1N1B4_3411 0.44 +0.1 3,802,820 + Pf1N1B4_3411 0.47 +0.6 3,802,820 + Pf1N1B4_3411 0.47 +0.6 3,802,820 + Pf1N1B4_3411 0.47 +0.1 3,802,946 + Pf1N1B4_3411 0.86 -0.3 3,802,947 - Pf1N1B4_3411 0.86 +1.1 3,802,947 - Pf1N1B4_3411 0.86 +0.6 3,802,960 + -0.3 3,802,964 - +0.1 3,802,964 - +1.5 3,803,051 - +2.2 3,803,105 + Pf1N1B4_3412 0.17 +0.8 3,803,105 - Pf1N1B4_3412 0.17 -0.5 3,803,168 + Pf1N1B4_3412 0.25 -1.5 3,803,579 + Pf1N1B4_3412 0.83 -0.6 3,803,579 + Pf1N1B4_3412 0.83 -1.5 3,803,579 + Pf1N1B4_3412 0.83 -0.1 3,803,579 + Pf1N1B4_3412 0.83 +0.5 3,803,579 + Pf1N1B4_3412 0.83 -1.1 3,803,580 - Pf1N1B4_3412 0.84 -1.2 3,803,580 - Pf1N1B4_3412 0.84 -0.9 3,803,580 - Pf1N1B4_3412 0.84 -1.8 3,803,580 - Pf1N1B4_3412 0.84 -2.1 3,803,580 - Pf1N1B4_3412 0.84 -0.5 3,803,734 + -2.1 3,803,734 + -1.1 3,803,734 + -0.9 3,803,734 + +0.4 3,803,734 + +2.1 3,803,735 - -1.3 3,803,735 - +0.1 3,803,735 - +1.8 3,803,735 - -1.8 3,803,735 - -1.8 3,803,735 - +0.8 3,803,735 - +0.3 3,803,735 - +0.1 3,803,808 - +0.1 3,804,308 + Pf1N1B4_3413 0.61 +0.2 3,804,308 + Pf1N1B4_3413 0.61 -2.3 3,804,439 + Pf1N1B4_3413 0.76 +1.4 3,804,465 + Pf1N1B4_3413 0.79 +0.6 3,804,465 + +1.5
Or see this region's nucleotide sequence