Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3333 and Pf1N1B4_3334 are separated by 4 nucleotides Pf1N1B4_3334 and Pf1N1B4_3335 are separated by 28 nucleotides Pf1N1B4_3335 and Pf1N1B4_5220 are separated by 83 nucleotides
Pf1N1B4_3333 - Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor, at 3,720,689 to 3,721,804
_3333
Pf1N1B4_3334 - Metal-dependent hydrolases of the beta-lactamase superfamily I, at 3,721,809 to 3,722,567
_3334
Pf1N1B4_3335 - Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), at 3,722,596 to 3,723,309
_3335
Pf1N1B4_5220 - tRNA-Pseudo-CGA, at 3,723,393 to 3,723,479
_5220
Position (kb)
3721
3722
3723 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3720.917 kb on + strand, within Pf1N1B4_3333 at 3720.917 kb on + strand, within Pf1N1B4_3333 at 3720.917 kb on + strand, within Pf1N1B4_3333 at 3720.917 kb on + strand, within Pf1N1B4_3333 at 3720.918 kb on - strand, within Pf1N1B4_3333 at 3720.918 kb on - strand, within Pf1N1B4_3333 at 3720.968 kb on + strand, within Pf1N1B4_3333 at 3720.968 kb on + strand, within Pf1N1B4_3333 at 3720.969 kb on - strand, within Pf1N1B4_3333 at 3720.969 kb on - strand, within Pf1N1B4_3333 at 3721.029 kb on + strand, within Pf1N1B4_3333 at 3721.029 kb on + strand, within Pf1N1B4_3333 at 3721.029 kb on + strand, within Pf1N1B4_3333 at 3721.029 kb on + strand, within Pf1N1B4_3333 at 3721.029 kb on + strand, within Pf1N1B4_3333 at 3721.029 kb on + strand, within Pf1N1B4_3333 at 3721.029 kb on + strand, within Pf1N1B4_3333 at 3721.029 kb on + strand, within Pf1N1B4_3333 at 3721.030 kb on - strand, within Pf1N1B4_3333 at 3721.244 kb on + strand, within Pf1N1B4_3333 at 3721.244 kb on + strand, within Pf1N1B4_3333 at 3721.565 kb on + strand, within Pf1N1B4_3333 at 3721.565 kb on + strand, within Pf1N1B4_3333 at 3721.565 kb on + strand, within Pf1N1B4_3333 at 3721.565 kb on + strand, within Pf1N1B4_3333 at 3721.565 kb on + strand, within Pf1N1B4_3333 at 3721.565 kb on + strand, within Pf1N1B4_3333 at 3721.565 kb on + strand, within Pf1N1B4_3333 at 3721.566 kb on - strand, within Pf1N1B4_3333 at 3721.568 kb on + strand, within Pf1N1B4_3333 at 3721.568 kb on + strand, within Pf1N1B4_3333 at 3721.568 kb on + strand, within Pf1N1B4_3333 at 3721.568 kb on + strand, within Pf1N1B4_3333 at 3721.568 kb on + strand, within Pf1N1B4_3333 at 3721.568 kb on + strand, within Pf1N1B4_3333 at 3721.568 kb on + strand, within Pf1N1B4_3333 at 3721.569 kb on - strand, within Pf1N1B4_3333 at 3721.569 kb on - strand at 3721.569 kb on - strand, within Pf1N1B4_3333 at 3721.569 kb on - strand, within Pf1N1B4_3333 at 3721.569 kb on - strand, within Pf1N1B4_3333 at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.875 kb on + strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.876 kb on - strand at 3721.879 kb on + strand at 3721.879 kb on + strand at 3721.880 kb on - strand at 3721.880 kb on - strand at 3722.037 kb on + strand, within Pf1N1B4_3334 at 3722.037 kb on + strand, within Pf1N1B4_3334 at 3722.038 kb on - strand, within Pf1N1B4_3334 at 3722.178 kb on + strand, within Pf1N1B4_3334 at 3722.178 kb on + strand, within Pf1N1B4_3334 at 3722.178 kb on + strand, within Pf1N1B4_3334 at 3722.178 kb on + strand, within Pf1N1B4_3334 at 3722.178 kb on + strand, within Pf1N1B4_3334 at 3722.178 kb on + strand, within Pf1N1B4_3334 at 3722.178 kb on + strand, within Pf1N1B4_3334 at 3722.232 kb on + strand, within Pf1N1B4_3334 at 3722.574 kb on + strand at 3722.574 kb on + strand at 3722.574 kb on + strand at 3722.574 kb on + strand at 3722.574 kb on + strand at 3722.574 kb on + strand at 3722.574 kb on + strand at 3722.574 kb on + strand at 3722.575 kb on - strand at 3722.578 kb on + strand at 3722.578 kb on + strand at 3722.578 kb on + strand at 3722.578 kb on + strand at 3722.578 kb on + strand at 3722.578 kb on + strand at 3722.579 kb on + strand at 3722.877 kb on + strand, within Pf1N1B4_3335 at 3722.941 kb on + strand, within Pf1N1B4_3335 at 3722.942 kb on - strand, within Pf1N1B4_3335 at 3723.327 kb on + strand at 3723.327 kb on + strand at 3723.377 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.485 kb on + strand at 3723.487 kb on + strand at 3723.487 kb on + strand at 3723.487 kb on + strand at 3723.487 kb on + strand at 3723.499 kb on + strand at 3723.511 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 3,720,917 + Pf1N1B4_3333 0.20 +0.1 3,720,917 + Pf1N1B4_3333 0.20 +0.8 3,720,917 + Pf1N1B4_3333 0.20 -2.4 3,720,917 + Pf1N1B4_3333 0.20 -1.5 3,720,918 - Pf1N1B4_3333 0.21 -0.7 3,720,918 - Pf1N1B4_3333 0.21 +0.5 3,720,968 + Pf1N1B4_3333 0.25 +0.5 3,720,968 + Pf1N1B4_3333 0.25 +0.6 3,720,969 - Pf1N1B4_3333 0.25 +0.1 3,720,969 - Pf1N1B4_3333 0.25 -2.1 3,721,029 + Pf1N1B4_3333 0.30 -0.7 3,721,029 + Pf1N1B4_3333 0.30 -2.1 3,721,029 + Pf1N1B4_3333 0.30 -1.5 3,721,029 + Pf1N1B4_3333 0.30 +1.1 3,721,029 + Pf1N1B4_3333 0.30 -1.5 3,721,029 + Pf1N1B4_3333 0.30 -1.9 3,721,029 + Pf1N1B4_3333 0.30 -1.0 3,721,029 + Pf1N1B4_3333 0.30 -3.2 3,721,030 - Pf1N1B4_3333 0.31 +0.0 3,721,244 + Pf1N1B4_3333 0.50 +0.5 3,721,244 + Pf1N1B4_3333 0.50 +1.4 3,721,565 + Pf1N1B4_3333 0.78 -1.8 3,721,565 + Pf1N1B4_3333 0.78 -0.1 3,721,565 + Pf1N1B4_3333 0.78 -0.8 3,721,565 + Pf1N1B4_3333 0.78 +1.6 3,721,565 + Pf1N1B4_3333 0.78 -1.7 3,721,565 + Pf1N1B4_3333 0.78 -0.3 3,721,565 + Pf1N1B4_3333 0.78 -0.1 3,721,566 - Pf1N1B4_3333 0.79 -1.5 3,721,568 + Pf1N1B4_3333 0.79 -0.0 3,721,568 + Pf1N1B4_3333 0.79 +0.3 3,721,568 + Pf1N1B4_3333 0.79 -1.7 3,721,568 + Pf1N1B4_3333 0.79 -0.5 3,721,568 + Pf1N1B4_3333 0.79 +0.1 3,721,568 + Pf1N1B4_3333 0.79 +0.1 3,721,568 + Pf1N1B4_3333 0.79 -0.9 3,721,569 - Pf1N1B4_3333 0.79 +2.8 3,721,569 - +0.1 3,721,569 - Pf1N1B4_3333 0.79 -0.2 3,721,569 - Pf1N1B4_3333 0.79 -3.0 3,721,569 - Pf1N1B4_3333 0.79 +0.7 3,721,875 + -1.5 3,721,875 + -1.5 3,721,875 + -0.9 3,721,875 + -1.5 3,721,875 + +0.4 3,721,875 + -1.7 3,721,875 + +0.9 3,721,875 + -1.5 3,721,875 + -1.2 3,721,875 + -0.0 3,721,875 + -1.9 3,721,875 + +1.1 3,721,875 + +1.7 3,721,875 + +0.5 3,721,875 + -1.5 3,721,875 + +0.1 3,721,875 + +0.9 3,721,875 + +0.3 3,721,875 + -1.6 3,721,875 + +0.3 3,721,875 + -0.2 3,721,875 + -0.5 3,721,875 + -0.7 3,721,875 + -0.5 3,721,875 + -1.5 3,721,875 + -0.6 3,721,875 + -0.6 3,721,875 + +0.4 3,721,875 + +0.4 3,721,876 - +0.9 3,721,876 - -1.5 3,721,876 - -0.6 3,721,876 - +0.1 3,721,876 - -1.0 3,721,876 - -0.2 3,721,876 - -0.9 3,721,876 - +0.7 3,721,876 - +0.4 3,721,876 - -0.4 3,721,876 - -0.4 3,721,876 - +0.3 3,721,876 - +0.6 3,721,876 - +0.5 3,721,876 - -0.9 3,721,876 - -0.5 3,721,879 + -0.4 3,721,879 + +1.1 3,721,880 - -0.9 3,721,880 - +0.1 3,722,037 + Pf1N1B4_3334 0.30 -2.0 3,722,037 + Pf1N1B4_3334 0.30 -0.2 3,722,038 - Pf1N1B4_3334 0.30 -1.2 3,722,178 + Pf1N1B4_3334 0.49 -0.9 3,722,178 + Pf1N1B4_3334 0.49 -1.9 3,722,178 + Pf1N1B4_3334 0.49 -1.2 3,722,178 + Pf1N1B4_3334 0.49 -0.9 3,722,178 + Pf1N1B4_3334 0.49 -1.2 3,722,178 + Pf1N1B4_3334 0.49 -1.2 3,722,178 + Pf1N1B4_3334 0.49 -2.3 3,722,232 + Pf1N1B4_3334 0.56 -0.9 3,722,574 + -0.0 3,722,574 + -1.2 3,722,574 + +1.1 3,722,574 + -0.8 3,722,574 + -0.5 3,722,574 + +0.1 3,722,574 + +0.1 3,722,574 + +1.1 3,722,575 - -0.2 3,722,578 + -0.6 3,722,578 + -1.2 3,722,578 + -1.5 3,722,578 + +1.7 3,722,578 + +0.5 3,722,578 + +1.3 3,722,579 + -1.6 3,722,877 + Pf1N1B4_3335 0.39 +0.4 3,722,941 + Pf1N1B4_3335 0.48 -0.9 3,722,942 - Pf1N1B4_3335 0.48 +0.1 3,723,327 + -2.2 3,723,327 + -0.6 3,723,377 + -0.2 3,723,485 + -1.2 3,723,485 + -0.3 3,723,485 + -1.1 3,723,485 + +1.8 3,723,485 + +2.1 3,723,485 + +0.4 3,723,485 + +2.5 3,723,487 + +1.1 3,723,487 + -0.0 3,723,487 + -0.0 3,723,487 + -0.9 3,723,499 + -0.7 3,723,511 + +1.1
Or see this region's nucleotide sequence