Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3183 and Pf1N1B4_3184 are separated by 145 nucleotides Pf1N1B4_3184 and Pf1N1B4_3185 are separated by 199 nucleotides Pf1N1B4_3185 and Pf1N1B4_3186 are separated by 5 nucleotides
Pf1N1B4_3183 - Glutathione S-transferase (EC 2.5.1.18), at 3,567,569 to 3,568,213
_3183
Pf1N1B4_3184 - Isochorismatase (EC 3.3.2.1), at 3,568,359 to 3,568,916
_3184
Pf1N1B4_3185 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,569,116 to 3,569,325
_3185
Pf1N1B4_3186 - Conserved secreted protein, at 3,569,331 to 3,569,816
_3186
Position (kb)
3568
3569 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3567.379 kb on + strand at 3567.379 kb on + strand at 3567.379 kb on + strand at 3567.380 kb on - strand at 3567.388 kb on + strand at 3567.431 kb on + strand at 3567.431 kb on + strand at 3567.432 kb on - strand at 3567.606 kb on + strand at 3567.607 kb on - strand at 3567.656 kb on + strand, within Pf1N1B4_3183 at 3567.656 kb on + strand, within Pf1N1B4_3183 at 3567.656 kb on + strand, within Pf1N1B4_3183 at 3567.656 kb on + strand, within Pf1N1B4_3183 at 3567.656 kb on + strand, within Pf1N1B4_3183 at 3567.656 kb on + strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.657 kb on - strand, within Pf1N1B4_3183 at 3567.714 kb on + strand, within Pf1N1B4_3183 at 3567.715 kb on - strand, within Pf1N1B4_3183 at 3567.738 kb on + strand, within Pf1N1B4_3183 at 3567.816 kb on + strand, within Pf1N1B4_3183 at 3567.817 kb on - strand, within Pf1N1B4_3183 at 3567.817 kb on - strand, within Pf1N1B4_3183 at 3567.981 kb on + strand, within Pf1N1B4_3183 at 3568.137 kb on + strand, within Pf1N1B4_3183 at 3568.138 kb on - strand, within Pf1N1B4_3183 at 3568.197 kb on + strand at 3568.198 kb on - strand at 3568.200 kb on + strand at 3568.200 kb on + strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.201 kb on - strand at 3568.245 kb on + strand at 3568.302 kb on - strand at 3568.787 kb on + strand, within Pf1N1B4_3184 at 3568.787 kb on + strand, within Pf1N1B4_3184 at 3568.788 kb on - strand, within Pf1N1B4_3184 at 3568.806 kb on + strand, within Pf1N1B4_3184 at 3568.807 kb on - strand, within Pf1N1B4_3184 at 3568.872 kb on + strand at 3568.872 kb on + strand at 3568.914 kb on + strand at 3568.954 kb on + strand at 3568.954 kb on + strand at 3569.013 kb on + strand at 3569.013 kb on + strand at 3569.025 kb on + strand at 3569.344 kb on - strand at 3569.358 kb on + strand at 3569.386 kb on + strand, within Pf1N1B4_3186 at 3569.386 kb on + strand, within Pf1N1B4_3186 at 3569.386 kb on + strand, within Pf1N1B4_3186 at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.387 kb on - strand at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.387 kb on - strand, within Pf1N1B4_3186 at 3569.401 kb on - strand, within Pf1N1B4_3186 at 3569.401 kb on - strand, within Pf1N1B4_3186 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.514 kb on - strand, within Pf1N1B4_3186 at 3569.519 kb on - strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.794 kb on - strand at 3569.824 kb on + strand at 3569.825 kb on - strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.879 kb on + strand at 3569.879 kb on + strand at 3569.880 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 3,567,379 + +1.2 3,567,379 + -1.5 3,567,379 + -0.6 3,567,380 - +0.6 3,567,388 + +2.2 3,567,431 + -2.4 3,567,431 + +1.7 3,567,432 - +1.2 3,567,606 + +0.4 3,567,607 - +0.3 3,567,656 + Pf1N1B4_3183 0.13 +1.9 3,567,656 + Pf1N1B4_3183 0.13 +0.4 3,567,656 + Pf1N1B4_3183 0.13 -0.8 3,567,656 + Pf1N1B4_3183 0.13 -0.8 3,567,656 + Pf1N1B4_3183 0.13 +1.3 3,567,656 + Pf1N1B4_3183 0.13 -0.1 3,567,657 - Pf1N1B4_3183 0.14 -0.4 3,567,657 - Pf1N1B4_3183 0.14 -1.8 3,567,657 - Pf1N1B4_3183 0.14 +1.4 3,567,657 - Pf1N1B4_3183 0.14 -1.4 3,567,657 - Pf1N1B4_3183 0.14 +1.9 3,567,657 - Pf1N1B4_3183 0.14 -0.8 3,567,657 - Pf1N1B4_3183 0.14 +2.5 3,567,657 - Pf1N1B4_3183 0.14 +0.9 3,567,657 - Pf1N1B4_3183 0.14 +0.2 3,567,657 - Pf1N1B4_3183 0.14 -1.5 3,567,714 + Pf1N1B4_3183 0.22 +0.2 3,567,715 - Pf1N1B4_3183 0.23 +1.0 3,567,738 + Pf1N1B4_3183 0.26 +0.6 3,567,816 + Pf1N1B4_3183 0.38 +0.0 3,567,817 - Pf1N1B4_3183 0.38 +0.8 3,567,817 - Pf1N1B4_3183 0.38 -0.6 3,567,981 + Pf1N1B4_3183 0.64 -0.8 3,568,137 + Pf1N1B4_3183 0.88 -0.1 3,568,138 - Pf1N1B4_3183 0.88 +0.7 3,568,197 + +0.1 3,568,198 - -0.1 3,568,200 + -0.8 3,568,200 + +0.3 3,568,201 - -2.1 3,568,201 - -0.1 3,568,201 - -0.2 3,568,201 - -0.8 3,568,201 - -0.8 3,568,201 - -0.5 3,568,201 - +1.2 3,568,245 + -0.4 3,568,302 - -0.1 3,568,787 + Pf1N1B4_3184 0.77 -1.6 3,568,787 + Pf1N1B4_3184 0.77 +0.9 3,568,788 - Pf1N1B4_3184 0.77 -1.1 3,568,806 + Pf1N1B4_3184 0.80 -1.1 3,568,807 - Pf1N1B4_3184 0.80 -1.0 3,568,872 + -1.4 3,568,872 + +0.2 3,568,914 + -1.0 3,568,954 + -0.8 3,568,954 + -1.2 3,569,013 + -0.4 3,569,013 + -1.4 3,569,025 + -1.8 3,569,344 - +0.2 3,569,358 + -0.8 3,569,386 + Pf1N1B4_3186 0.11 +0.2 3,569,386 + Pf1N1B4_3186 0.11 -1.1 3,569,386 + Pf1N1B4_3186 0.11 +0.1 3,569,387 - Pf1N1B4_3186 0.12 +1.2 3,569,387 - Pf1N1B4_3186 0.12 +0.7 3,569,387 - -0.1 3,569,387 - Pf1N1B4_3186 0.12 +0.0 3,569,387 - Pf1N1B4_3186 0.12 -0.8 3,569,401 - Pf1N1B4_3186 0.14 -0.4 3,569,401 - Pf1N1B4_3186 0.14 -0.4 3,569,513 + Pf1N1B4_3186 0.37 +0.2 3,569,513 + Pf1N1B4_3186 0.37 +0.5 3,569,514 - Pf1N1B4_3186 0.38 -1.0 3,569,519 - Pf1N1B4_3186 0.39 +2.8 3,569,577 + Pf1N1B4_3186 0.51 -0.4 3,569,577 + Pf1N1B4_3186 0.51 -0.2 3,569,577 + Pf1N1B4_3186 0.51 -0.8 3,569,577 + Pf1N1B4_3186 0.51 +0.7 3,569,577 + Pf1N1B4_3186 0.51 -0.0 3,569,577 + Pf1N1B4_3186 0.51 +2.5 3,569,577 + Pf1N1B4_3186 0.51 +0.2 3,569,577 + Pf1N1B4_3186 0.51 -0.4 3,569,577 + Pf1N1B4_3186 0.51 +0.2 3,569,577 + Pf1N1B4_3186 0.51 -1.6 3,569,577 + Pf1N1B4_3186 0.51 -2.0 3,569,577 + Pf1N1B4_3186 0.51 +2.2 3,569,577 + Pf1N1B4_3186 0.51 +0.7 3,569,577 + Pf1N1B4_3186 0.51 +0.7 3,569,577 + Pf1N1B4_3186 0.51 +2.0 3,569,578 - Pf1N1B4_3186 0.51 +0.2 3,569,578 - Pf1N1B4_3186 0.51 -1.1 3,569,578 - Pf1N1B4_3186 0.51 +0.5 3,569,578 - Pf1N1B4_3186 0.51 +0.3 3,569,578 - Pf1N1B4_3186 0.51 -1.8 3,569,578 - Pf1N1B4_3186 0.51 +0.6 3,569,578 - Pf1N1B4_3186 0.51 +0.7 3,569,578 - Pf1N1B4_3186 0.51 -0.1 3,569,676 + Pf1N1B4_3186 0.71 +0.3 3,569,676 + Pf1N1B4_3186 0.71 +0.5 3,569,793 + -0.2 3,569,793 + +0.2 3,569,793 + -1.1 3,569,793 + +0.4 3,569,794 - -1.5 3,569,824 + +0.5 3,569,825 - -1.1 3,569,845 + -1.6 3,569,845 + -0.3 3,569,845 + -1.4 3,569,879 + -0.6 3,569,879 + -0.1 3,569,880 - +0.8
Or see this region's nucleotide sequence