Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3073 and Pf1N1B4_3074 are separated by 282 nucleotides Pf1N1B4_3074 and Pf1N1B4_3075 are separated by 119 nucleotides Pf1N1B4_3075 and Pf1N1B4_3076 overlap by 4 nucleotides
Pf1N1B4_3073 - probable glucosyl transferase, at 3,454,364 to 3,456,955
_3073
Pf1N1B4_3074 - HesA/MoeB/ThiF family protein related to EC-YgdL, at 3,457,238 to 3,458,053
_3074
Pf1N1B4_3075 - Cysteine desulfurase CsdA-CsdE, sulfur acceptor protein CsdE, at 3,458,173 to 3,458,577
_3075
Pf1N1B4_3076 - Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA, at 3,458,574 to 3,459,779
_3076
Position (kb)
3457
3458
3459 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3456.711 kb on + strand at 3456.955 kb on + strand at 3456.955 kb on + strand at 3456.955 kb on + strand at 3456.955 kb on + strand at 3456.955 kb on + strand at 3456.956 kb on - strand at 3456.956 kb on - strand at 3456.956 kb on - strand at 3456.956 kb on - strand at 3456.956 kb on - strand at 3456.959 kb on + strand at 3456.960 kb on - strand at 3456.960 kb on - strand at 3456.960 kb on - strand at 3457.033 kb on + strand at 3457.034 kb on - strand at 3457.034 kb on - strand at 3457.034 kb on - strand at 3457.034 kb on - strand at 3457.101 kb on + strand at 3457.101 kb on + strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.139 kb on - strand at 3457.146 kb on + strand at 3457.154 kb on + strand at 3457.154 kb on + strand at 3457.154 kb on + strand at 3457.155 kb on - strand at 3457.155 kb on - strand at 3457.155 kb on - strand at 3457.212 kb on + strand at 3457.213 kb on - strand at 3457.213 kb on - strand at 3457.213 kb on - strand at 3457.213 kb on - strand at 3457.215 kb on - strand at 3457.215 kb on - strand at 3457.249 kb on + strand at 3457.286 kb on + strand at 3457.286 kb on + strand at 3457.286 kb on + strand at 3457.287 kb on - strand at 3457.577 kb on + strand, within Pf1N1B4_3074 at 3457.578 kb on - strand, within Pf1N1B4_3074 at 3457.823 kb on + strand, within Pf1N1B4_3074 at 3457.823 kb on + strand, within Pf1N1B4_3074 at 3458.200 kb on + strand at 3458.263 kb on - strand at 3458.641 kb on + strand at 3458.641 kb on + strand at 3458.641 kb on + strand at 3458.641 kb on + strand at 3458.642 kb on - strand at 3458.642 kb on - strand at 3458.765 kb on + strand, within Pf1N1B4_3076 at 3458.766 kb on - strand, within Pf1N1B4_3076 at 3458.857 kb on + strand, within Pf1N1B4_3076 at 3458.857 kb on + strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3459.008 kb on - strand, within Pf1N1B4_3076
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 3,456,711 + -0.8 3,456,955 + -1.8 3,456,955 + +0.4 3,456,955 + -1.1 3,456,955 + -0.4 3,456,955 + -0.8 3,456,956 - +2.6 3,456,956 - +0.6 3,456,956 - -1.4 3,456,956 - -1.1 3,456,956 - +1.1 3,456,959 + +0.1 3,456,960 - -2.1 3,456,960 - -0.8 3,456,960 - -0.9 3,457,033 + -0.4 3,457,034 - -1.2 3,457,034 - -1.5 3,457,034 - +1.6 3,457,034 - +1.7 3,457,101 + -0.5 3,457,101 + -1.6 3,457,102 - +0.4 3,457,102 - -0.1 3,457,102 - -0.9 3,457,102 - +0.6 3,457,102 - -1.2 3,457,102 - +0.2 3,457,139 - +0.2 3,457,146 + +0.1 3,457,154 + +1.0 3,457,154 + -1.1 3,457,154 + +1.0 3,457,155 - +2.0 3,457,155 - +0.8 3,457,155 - +1.0 3,457,212 + -1.4 3,457,213 - +0.2 3,457,213 - -0.8 3,457,213 - -0.0 3,457,213 - +0.8 3,457,215 - -0.3 3,457,215 - -0.1 3,457,249 + +0.2 3,457,286 + -1.9 3,457,286 + +0.2 3,457,286 + +1.2 3,457,287 - +1.6 3,457,577 + Pf1N1B4_3074 0.42 -1.9 3,457,578 - Pf1N1B4_3074 0.42 -0.6 3,457,823 + Pf1N1B4_3074 0.72 +0.5 3,457,823 + Pf1N1B4_3074 0.72 -2.6 3,458,200 + +0.6 3,458,263 - +1.5 3,458,641 + -1.1 3,458,641 + -0.4 3,458,641 + -0.4 3,458,641 + -1.4 3,458,642 - +0.0 3,458,642 - +0.2 3,458,765 + Pf1N1B4_3076 0.16 -1.4 3,458,766 - Pf1N1B4_3076 0.16 +1.0 3,458,857 + Pf1N1B4_3076 0.23 -0.4 3,458,857 + Pf1N1B4_3076 0.23 +1.1 3,458,858 - Pf1N1B4_3076 0.24 +2.8 3,458,858 - Pf1N1B4_3076 0.24 -1.4 3,458,858 - Pf1N1B4_3076 0.24 +0.8 3,458,858 - Pf1N1B4_3076 0.24 -0.2 3,458,858 - Pf1N1B4_3076 0.24 -1.2 3,459,008 - Pf1N1B4_3076 0.36 +1.8
Or see this region's nucleotide sequence