Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2822 and Pf1N1B4_2823 overlap by 4 nucleotides Pf1N1B4_2823 and Pf1N1B4_2824 are separated by 129 nucleotides
Pf1N1B4_2822 - Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70), at 3,165,270 to 3,166,190
_2822
Pf1N1B4_2823 - COG1496: Uncharacterized conserved protein, at 3,166,187 to 3,166,912
_2823
Pf1N1B4_2824 - ClpB protein, at 3,167,042 to 3,169,606
_2824
Position (kb)
3166
3167 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3165.218 kb on + strand at 3165.218 kb on + strand at 3165.487 kb on - strand, within Pf1N1B4_2822 at 3166.363 kb on + strand at 3166.763 kb on + strand, within Pf1N1B4_2823 at 3166.763 kb on + strand, within Pf1N1B4_2823 at 3166.763 kb on + strand, within Pf1N1B4_2823 at 3166.780 kb on - strand, within Pf1N1B4_2823 at 3166.812 kb on - strand, within Pf1N1B4_2823 at 3166.910 kb on + strand at 3166.910 kb on + strand at 3166.970 kb on + strand at 3166.970 kb on + strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3166.971 kb on - strand at 3167.008 kb on + strand at 3167.008 kb on + strand at 3167.009 kb on - strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.010 kb on + strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.011 kb on - strand at 3167.047 kb on + strand at 3167.047 kb on + strand at 3167.048 kb on - strand at 3167.048 kb on - strand at 3167.048 kb on - strand at 3167.049 kb on + strand at 3167.049 kb on + strand at 3167.049 kb on + strand at 3167.049 kb on + strand at 3167.050 kb on - strand at 3167.076 kb on + strand at 3167.224 kb on + strand at 3167.244 kb on + strand at 3167.245 kb on - strand at 3167.302 kb on - strand, within Pf1N1B4_2824 at 3167.302 kb on - strand, within Pf1N1B4_2824 at 3167.302 kb on - strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.531 kb on + strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824 at 3167.532 kb on - strand, within Pf1N1B4_2824
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 3,165,218 + -1.3 3,165,218 + -1.4 3,165,487 - Pf1N1B4_2822 0.24 -1.7 3,166,363 + -0.7 3,166,763 + Pf1N1B4_2823 0.79 -0.7 3,166,763 + Pf1N1B4_2823 0.79 -0.3 3,166,763 + Pf1N1B4_2823 0.79 -1.3 3,166,780 - Pf1N1B4_2823 0.82 -1.5 3,166,812 - Pf1N1B4_2823 0.86 -1.0 3,166,910 + -0.3 3,166,910 + -0.3 3,166,970 + -2.0 3,166,970 + +0.3 3,166,971 - +0.5 3,166,971 - +2.3 3,166,971 - +1.3 3,166,971 - -0.0 3,166,971 - +2.5 3,166,971 - -1.3 3,166,971 - -0.6 3,166,971 - -1.3 3,167,008 + -1.6 3,167,008 + +0.1 3,167,009 - -0.2 3,167,010 + -1.9 3,167,010 + -1.8 3,167,010 + +0.7 3,167,010 + +0.7 3,167,010 + +0.5 3,167,010 + -0.3 3,167,010 + -0.0 3,167,011 - -0.1 3,167,011 - -0.4 3,167,011 - -0.7 3,167,011 - -1.5 3,167,011 - -1.7 3,167,011 - +0.3 3,167,047 + -0.8 3,167,047 + +1.3 3,167,048 - -0.7 3,167,048 - -0.7 3,167,048 - -0.5 3,167,049 + -0.7 3,167,049 + -3.3 3,167,049 + +0.3 3,167,049 + -1.4 3,167,050 - +0.3 3,167,076 + -1.5 3,167,224 + -2.3 3,167,244 + -0.7 3,167,245 - +0.7 3,167,302 - Pf1N1B4_2824 0.10 -0.4 3,167,302 - Pf1N1B4_2824 0.10 +0.7 3,167,302 - Pf1N1B4_2824 0.10 +0.2 3,167,531 + Pf1N1B4_2824 0.19 +1.6 3,167,531 + Pf1N1B4_2824 0.19 -0.4 3,167,531 + Pf1N1B4_2824 0.19 +0.2 3,167,531 + Pf1N1B4_2824 0.19 -1.8 3,167,531 + Pf1N1B4_2824 0.19 -0.6 3,167,531 + Pf1N1B4_2824 0.19 -1.3 3,167,532 - Pf1N1B4_2824 0.19 +1.3 3,167,532 - Pf1N1B4_2824 0.19 -1.7 3,167,532 - Pf1N1B4_2824 0.19 +0.3 3,167,532 - Pf1N1B4_2824 0.19 +0.2 3,167,532 - Pf1N1B4_2824 0.19 +2.1 3,167,532 - Pf1N1B4_2824 0.19 -1.7
Or see this region's nucleotide sequence