Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2451 and Pf1N1B4_2452 are separated by 131 nucleotides Pf1N1B4_2452 and Pf1N1B4_2453 overlap by 11 nucleotides Pf1N1B4_2453 and Pf1N1B4_2454 are separated by 192 nucleotides
Pf1N1B4_2451 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 2,781,464 to 2,782,504
_2451
Pf1N1B4_2452 - Membrane protein, putative, at 2,782,636 to 2,783,352
_2452
Pf1N1B4_2453 - hypothetical protein, at 2,783,342 to 2,783,473
_2453
Pf1N1B4_2454 - Serine hydroxymethyltransferase (EC 2.1.2.1) (from data) , at 2,783,666 to 2,784,919
_2454
Position (kb)
2783
2784 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2782.401 kb on + strand at 2782.460 kb on - strand at 2782.467 kb on + strand at 2782.467 kb on + strand at 2782.468 kb on - strand at 2782.468 kb on - strand at 2782.468 kb on - strand at 2782.530 kb on + strand at 2782.530 kb on + strand at 2782.530 kb on + strand at 2782.530 kb on + strand at 2782.531 kb on - strand at 2782.531 kb on - strand at 2782.616 kb on + strand at 2782.635 kb on + strand at 2782.635 kb on + strand at 2782.635 kb on + strand at 2782.635 kb on + strand at 2782.635 kb on + strand at 2782.635 kb on + strand at 2782.636 kb on - strand at 2782.636 kb on - strand at 2782.636 kb on - strand at 2782.636 kb on - strand at 2782.636 kb on - strand at 2782.717 kb on + strand, within Pf1N1B4_2452 at 2782.717 kb on + strand, within Pf1N1B4_2452 at 2782.718 kb on - strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.745 kb on - strand, within Pf1N1B4_2452 at 2782.745 kb on - strand, within Pf1N1B4_2452 at 2782.745 kb on - strand, within Pf1N1B4_2452 at 2782.745 kb on - strand, within Pf1N1B4_2452 at 2782.863 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.895 kb on - strand, within Pf1N1B4_2452 at 2782.895 kb on - strand, within Pf1N1B4_2452 at 2782.895 kb on - strand, within Pf1N1B4_2452 at 2782.916 kb on + strand, within Pf1N1B4_2452 at 2782.916 kb on + strand, within Pf1N1B4_2452 at 2782.917 kb on - strand, within Pf1N1B4_2452 at 2782.917 kb on - strand, within Pf1N1B4_2452 at 2783.091 kb on + strand, within Pf1N1B4_2452 at 2783.104 kb on + strand, within Pf1N1B4_2452 at 2783.162 kb on - strand, within Pf1N1B4_2452 at 2783.162 kb on - strand, within Pf1N1B4_2452 at 2783.164 kb on + strand, within Pf1N1B4_2452 at 2783.164 kb on + strand, within Pf1N1B4_2452 at 2783.164 kb on + strand, within Pf1N1B4_2452 at 2783.164 kb on + strand, within Pf1N1B4_2452 at 2783.293 kb on + strand at 2783.293 kb on + strand at 2783.302 kb on + strand at 2783.365 kb on + strand, within Pf1N1B4_2453 at 2783.365 kb on + strand, within Pf1N1B4_2453 at 2783.366 kb on - strand, within Pf1N1B4_2453 at 2783.583 kb on + strand at 2783.583 kb on + strand at 2783.584 kb on - strand at 2783.643 kb on + strand at 2783.643 kb on + strand at 2783.643 kb on + strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.777 kb on + strand at 2783.777 kb on + strand at 2783.777 kb on + strand at 2783.777 kb on + strand at 2783.777 kb on + strand at 2783.778 kb on - strand at 2783.778 kb on - strand at 2783.778 kb on - strand at 2783.778 kb on - strand at 2783.778 kb on - strand at 2783.828 kb on + strand, within Pf1N1B4_2454 at 2783.829 kb on - strand, within Pf1N1B4_2454 at 2783.858 kb on + strand at 2783.859 kb on - strand, within Pf1N1B4_2454 at 2783.934 kb on - strand, within Pf1N1B4_2454 at 2783.981 kb on + strand, within Pf1N1B4_2454 at 2783.982 kb on - strand, within Pf1N1B4_2454 at 2784.083 kb on + strand, within Pf1N1B4_2454 at 2784.083 kb on + strand, within Pf1N1B4_2454 at 2784.084 kb on - strand, within Pf1N1B4_2454 at 2784.084 kb on - strand, within Pf1N1B4_2454 at 2784.084 kb on - strand, within Pf1N1B4_2454 at 2784.099 kb on - strand, within Pf1N1B4_2454 at 2784.324 kb on - strand, within Pf1N1B4_2454
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 2,782,401 + +0.4 2,782,460 - -1.4 2,782,467 + +2.4 2,782,467 + -0.3 2,782,468 - +0.2 2,782,468 - +0.4 2,782,468 - +0.1 2,782,530 + -1.1 2,782,530 + -0.9 2,782,530 + +1.4 2,782,530 + -1.8 2,782,531 - +3.2 2,782,531 - -0.9 2,782,616 + -2.1 2,782,635 + -1.4 2,782,635 + +0.3 2,782,635 + +0.4 2,782,635 + -0.2 2,782,635 + -0.6 2,782,635 + -1.4 2,782,636 - +0.6 2,782,636 - -0.7 2,782,636 - +0.2 2,782,636 - +1.2 2,782,636 - -0.4 2,782,717 + Pf1N1B4_2452 0.11 -0.3 2,782,717 + Pf1N1B4_2452 0.11 +0.3 2,782,718 - Pf1N1B4_2452 0.11 -1.5 2,782,744 + Pf1N1B4_2452 0.15 -0.2 2,782,744 + Pf1N1B4_2452 0.15 -0.9 2,782,744 + Pf1N1B4_2452 0.15 -0.7 2,782,744 + Pf1N1B4_2452 0.15 +1.6 2,782,744 + Pf1N1B4_2452 0.15 +1.4 2,782,745 - Pf1N1B4_2452 0.15 +0.4 2,782,745 - Pf1N1B4_2452 0.15 -0.6 2,782,745 - Pf1N1B4_2452 0.15 +0.8 2,782,745 - Pf1N1B4_2452 0.15 +0.1 2,782,863 + Pf1N1B4_2452 0.32 -0.8 2,782,894 + Pf1N1B4_2452 0.36 -1.6 2,782,894 + Pf1N1B4_2452 0.36 +0.4 2,782,894 + Pf1N1B4_2452 0.36 +1.4 2,782,894 + Pf1N1B4_2452 0.36 -0.3 2,782,894 + Pf1N1B4_2452 0.36 +0.8 2,782,894 + Pf1N1B4_2452 0.36 -0.6 2,782,894 + Pf1N1B4_2452 0.36 -1.9 2,782,894 + Pf1N1B4_2452 0.36 +1.1 2,782,894 + Pf1N1B4_2452 0.36 -2.2 2,782,895 - Pf1N1B4_2452 0.36 +1.8 2,782,895 - Pf1N1B4_2452 0.36 -0.9 2,782,895 - Pf1N1B4_2452 0.36 -1.1 2,782,916 + Pf1N1B4_2452 0.39 -0.6 2,782,916 + Pf1N1B4_2452 0.39 -1.4 2,782,917 - Pf1N1B4_2452 0.39 +0.5 2,782,917 - Pf1N1B4_2452 0.39 +2.0 2,783,091 + Pf1N1B4_2452 0.63 -2.5 2,783,104 + Pf1N1B4_2452 0.65 -0.2 2,783,162 - Pf1N1B4_2452 0.73 +2.4 2,783,162 - Pf1N1B4_2452 0.73 -0.3 2,783,164 + Pf1N1B4_2452 0.74 -0.2 2,783,164 + Pf1N1B4_2452 0.74 +1.0 2,783,164 + Pf1N1B4_2452 0.74 -0.6 2,783,164 + Pf1N1B4_2452 0.74 +1.4 2,783,293 + +1.1 2,783,293 + +0.7 2,783,302 + -0.7 2,783,365 + Pf1N1B4_2453 0.17 +0.4 2,783,365 + Pf1N1B4_2453 0.17 -1.8 2,783,366 - Pf1N1B4_2453 0.18 -3.2 2,783,583 + +1.4 2,783,583 + +1.8 2,783,584 - +0.2 2,783,643 + -0.8 2,783,643 + +1.8 2,783,643 + -1.6 2,783,644 - +2.0 2,783,644 - -0.6 2,783,644 - +1.0 2,783,644 - -1.2 2,783,644 - -1.8 2,783,644 - +0.4 2,783,644 - -0.2 2,783,644 - +1.2 2,783,777 + +1.1 2,783,777 + -0.2 2,783,777 + -0.8 2,783,777 + +1.0 2,783,777 + -0.2 2,783,778 - +0.2 2,783,778 - -1.1 2,783,778 - -2.6 2,783,778 - -0.5 2,783,778 - +1.0 2,783,828 + Pf1N1B4_2454 0.13 +0.4 2,783,829 - Pf1N1B4_2454 0.13 -0.2 2,783,858 + -0.6 2,783,859 - Pf1N1B4_2454 0.15 -0.5 2,783,934 - Pf1N1B4_2454 0.21 +0.5 2,783,981 + Pf1N1B4_2454 0.25 -1.1 2,783,982 - Pf1N1B4_2454 0.25 -1.6 2,784,083 + Pf1N1B4_2454 0.33 -0.7 2,784,083 + Pf1N1B4_2454 0.33 +0.2 2,784,084 - Pf1N1B4_2454 0.33 -0.4 2,784,084 - Pf1N1B4_2454 0.33 -1.0 2,784,084 - Pf1N1B4_2454 0.33 +0.9 2,784,099 - Pf1N1B4_2454 0.35 -1.2 2,784,324 - Pf1N1B4_2454 0.52 -0.0
Or see this region's nucleotide sequence