Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2334 and Pf1N1B4_2335 overlap by 4 nucleotides Pf1N1B4_2335 and Pf1N1B4_2336 overlap by 4 nucleotides Pf1N1B4_2336 and Pf1N1B4_2337 are separated by 26 nucleotides
Pf1N1B4_2334 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 2,651,846 to 2,653,051
_2334
Pf1N1B4_2335 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 2,653,048 to 2,653,644
_2335
Pf1N1B4_2336 - FIG003551: hypothetical protein, at 2,653,641 to 2,654,075
_2336
Pf1N1B4_2337 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,102 to 2,654,722
_2337
Position (kb)
2653
2654 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2652.117 kb on + strand, within Pf1N1B4_2334 at 2652.118 kb on - strand, within Pf1N1B4_2334 at 2652.235 kb on + strand, within Pf1N1B4_2334 at 2652.279 kb on + strand at 2652.502 kb on + strand, within Pf1N1B4_2334 at 2652.822 kb on + strand, within Pf1N1B4_2334 at 2652.870 kb on + strand, within Pf1N1B4_2334 at 2652.870 kb on + strand, within Pf1N1B4_2334 at 2652.871 kb on - strand, within Pf1N1B4_2334 at 2652.921 kb on + strand, within Pf1N1B4_2334 at 2652.922 kb on - strand, within Pf1N1B4_2334 at 2652.957 kb on + strand at 2652.957 kb on + strand at 2652.957 kb on + strand at 2652.958 kb on - strand at 2652.958 kb on - strand at 2652.958 kb on - strand at 2653.125 kb on + strand, within Pf1N1B4_2335 at 2653.125 kb on + strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.126 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.389 kb on - strand, within Pf1N1B4_2335 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.756 kb on + strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.757 kb on - strand, within Pf1N1B4_2336 at 2653.759 kb on + strand, within Pf1N1B4_2336 at 2653.856 kb on + strand at 2653.857 kb on - strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.963 kb on + strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2653.964 kb on - strand, within Pf1N1B4_2336 at 2654.048 kb on + strand at 2654.049 kb on - strand at 2654.049 kb on - strand at 2654.052 kb on + strand at 2654.052 kb on + strand at 2654.053 kb on - strand at 2654.064 kb on + strand at 2654.064 kb on + strand at 2654.165 kb on + strand, within Pf1N1B4_2337 at 2654.174 kb on - strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.180 kb on + strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.181 kb on - strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.307 kb on + strand, within Pf1N1B4_2337 at 2654.308 kb on - strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand, within Pf1N1B4_2337 at 2654.316 kb on + strand at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.317 kb on - strand, within Pf1N1B4_2337 at 2654.451 kb on + strand, within Pf1N1B4_2337
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 2,652,117 + Pf1N1B4_2334 0.22 -0.2 2,652,118 - Pf1N1B4_2334 0.23 -1.4 2,652,235 + Pf1N1B4_2334 0.32 -0.2 2,652,279 + -0.2 2,652,502 + Pf1N1B4_2334 0.54 -0.8 2,652,822 + Pf1N1B4_2334 0.81 -0.6 2,652,870 + Pf1N1B4_2334 0.85 +0.7 2,652,870 + Pf1N1B4_2334 0.85 +2.2 2,652,871 - Pf1N1B4_2334 0.85 +1.4 2,652,921 + Pf1N1B4_2334 0.89 +0.3 2,652,922 - Pf1N1B4_2334 0.89 -1.4 2,652,957 + +0.4 2,652,957 + +3.0 2,652,957 + -0.6 2,652,958 - +1.4 2,652,958 - +1.0 2,652,958 - -2.0 2,653,125 + Pf1N1B4_2335 0.13 -1.2 2,653,125 + Pf1N1B4_2335 0.13 -0.4 2,653,126 - Pf1N1B4_2335 0.13 +0.4 2,653,126 - Pf1N1B4_2335 0.13 -0.2 2,653,126 - Pf1N1B4_2335 0.13 -1.9 2,653,126 - Pf1N1B4_2335 0.13 -1.2 2,653,389 - Pf1N1B4_2335 0.57 +0.0 2,653,389 - Pf1N1B4_2335 0.57 -1.5 2,653,389 - Pf1N1B4_2335 0.57 -1.2 2,653,756 + Pf1N1B4_2336 0.26 -1.2 2,653,756 + Pf1N1B4_2336 0.26 +1.7 2,653,756 + Pf1N1B4_2336 0.26 -0.0 2,653,756 + -0.2 2,653,756 + Pf1N1B4_2336 0.26 -0.9 2,653,756 + Pf1N1B4_2336 0.26 -1.9 2,653,756 + Pf1N1B4_2336 0.26 +1.0 2,653,757 - Pf1N1B4_2336 0.27 +0.4 2,653,757 - Pf1N1B4_2336 0.27 -0.2 2,653,759 + Pf1N1B4_2336 0.27 -1.2 2,653,856 + -0.6 2,653,857 - Pf1N1B4_2336 0.50 -1.6 2,653,963 + Pf1N1B4_2336 0.74 +1.4 2,653,963 + Pf1N1B4_2336 0.74 +0.1 2,653,963 + Pf1N1B4_2336 0.74 +0.4 2,653,963 + Pf1N1B4_2336 0.74 +0.2 2,653,963 + Pf1N1B4_2336 0.74 +0.7 2,653,963 + Pf1N1B4_2336 0.74 +0.3 2,653,964 - Pf1N1B4_2336 0.74 -0.6 2,653,964 - Pf1N1B4_2336 0.74 -1.2 2,654,048 + +2.4 2,654,049 - +0.8 2,654,049 - +0.4 2,654,052 + +0.3 2,654,052 + -1.4 2,654,053 - +2.6 2,654,064 + +0.4 2,654,064 + +0.3 2,654,165 + Pf1N1B4_2337 0.10 -0.9 2,654,174 - Pf1N1B4_2337 0.12 -0.6 2,654,180 + Pf1N1B4_2337 0.13 -2.7 2,654,180 + Pf1N1B4_2337 0.13 -0.9 2,654,180 + Pf1N1B4_2337 0.13 +1.2 2,654,180 + Pf1N1B4_2337 0.13 -0.2 2,654,181 - Pf1N1B4_2337 0.13 -2.8 2,654,181 - Pf1N1B4_2337 0.13 +1.0 2,654,307 + Pf1N1B4_2337 0.33 +0.0 2,654,307 + Pf1N1B4_2337 0.33 -0.9 2,654,307 + Pf1N1B4_2337 0.33 -1.6 2,654,308 - Pf1N1B4_2337 0.33 -0.9 2,654,316 + Pf1N1B4_2337 0.34 -1.2 2,654,316 + Pf1N1B4_2337 0.34 -1.7 2,654,316 + Pf1N1B4_2337 0.34 -1.2 2,654,316 + Pf1N1B4_2337 0.34 -0.9 2,654,316 + Pf1N1B4_2337 0.34 -1.6 2,654,316 + Pf1N1B4_2337 0.34 -2.8 2,654,316 + -0.6 2,654,317 - Pf1N1B4_2337 0.35 +0.6 2,654,317 - Pf1N1B4_2337 0.35 -0.9 2,654,451 + Pf1N1B4_2337 0.56 -1.2
Or see this region's nucleotide sequence