Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2000 and Pf1N1B4_2001 are separated by 147 nucleotides Pf1N1B4_2001 and Pf1N1B4_2002 are separated by 196 nucleotides
Pf1N1B4_2000 - Pyridoxal kinase (EC 2.7.1.35), at 2,282,231 to 2,283,103
_2000
Pf1N1B4_2001 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 2,283,251 to 2,283,733
_2001
Pf1N1B4_2002 - putative Glutathione-regulated potassium-efflux system protein KefB, at 2,283,930 to 2,285,693
_2002
Position (kb)
2283
2284 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2282.347 kb on + strand, within Pf1N1B4_2000 at 2282.368 kb on + strand, within Pf1N1B4_2000 at 2282.368 kb on + strand, within Pf1N1B4_2000 at 2282.368 kb on + strand, within Pf1N1B4_2000 at 2282.368 kb on + strand, within Pf1N1B4_2000 at 2282.368 kb on + strand, within Pf1N1B4_2000 at 2282.369 kb on - strand, within Pf1N1B4_2000 at 2282.369 kb on - strand, within Pf1N1B4_2000 at 2282.369 kb on - strand, within Pf1N1B4_2000 at 2282.376 kb on - strand, within Pf1N1B4_2000 at 2282.376 kb on - strand, within Pf1N1B4_2000 at 2282.427 kb on + strand, within Pf1N1B4_2000 at 2282.427 kb on + strand, within Pf1N1B4_2000 at 2282.427 kb on + strand, within Pf1N1B4_2000 at 2282.428 kb on - strand, within Pf1N1B4_2000 at 2282.428 kb on - strand, within Pf1N1B4_2000 at 2282.559 kb on + strand, within Pf1N1B4_2000 at 2282.559 kb on + strand, within Pf1N1B4_2000 at 2282.561 kb on + strand, within Pf1N1B4_2000 at 2282.561 kb on + strand, within Pf1N1B4_2000 at 2282.561 kb on + strand, within Pf1N1B4_2000 at 2282.561 kb on + strand, within Pf1N1B4_2000 at 2282.561 kb on + strand, within Pf1N1B4_2000 at 2282.561 kb on + strand, within Pf1N1B4_2000 at 2282.561 kb on + strand, within Pf1N1B4_2000 at 2282.562 kb on - strand, within Pf1N1B4_2000 at 2282.562 kb on - strand, within Pf1N1B4_2000 at 2282.562 kb on - strand, within Pf1N1B4_2000 at 2282.562 kb on - strand, within Pf1N1B4_2000 at 2282.562 kb on - strand, within Pf1N1B4_2000 at 2282.793 kb on - strand, within Pf1N1B4_2000 at 2283.023 kb on + strand at 2283.023 kb on + strand at 2283.023 kb on + strand at 2283.023 kb on + strand at 2283.023 kb on + strand at 2283.023 kb on + strand at 2283.024 kb on - strand at 2283.024 kb on - strand at 2283.159 kb on + strand at 2283.160 kb on - strand at 2283.160 kb on - strand at 2283.221 kb on + strand at 2283.221 kb on + strand at 2283.221 kb on + strand at 2283.221 kb on + strand at 2283.222 kb on - strand at 2283.222 kb on - strand at 2283.222 kb on - strand at 2283.222 kb on - strand at 2283.222 kb on - strand at 2283.247 kb on + strand at 2283.247 kb on + strand at 2283.247 kb on + strand at 2283.247 kb on + strand at 2283.248 kb on - strand at 2283.327 kb on + strand, within Pf1N1B4_2001 at 2283.327 kb on + strand, within Pf1N1B4_2001 at 2283.328 kb on - strand, within Pf1N1B4_2001 at 2283.328 kb on - strand, within Pf1N1B4_2001 at 2283.435 kb on - strand, within Pf1N1B4_2001 at 2283.501 kb on + strand, within Pf1N1B4_2001 at 2283.501 kb on + strand, within Pf1N1B4_2001 at 2283.501 kb on + strand, within Pf1N1B4_2001 at 2283.501 kb on + strand, within Pf1N1B4_2001 at 2283.502 kb on - strand, within Pf1N1B4_2001 at 2283.502 kb on - strand, within Pf1N1B4_2001 at 2283.502 kb on - strand, within Pf1N1B4_2001 at 2283.588 kb on + strand, within Pf1N1B4_2001 at 2283.589 kb on - strand, within Pf1N1B4_2001 at 2283.589 kb on - strand, within Pf1N1B4_2001 at 2283.618 kb on + strand, within Pf1N1B4_2001 at 2283.636 kb on + strand, within Pf1N1B4_2001 at 2283.738 kb on + strand at 2283.738 kb on + strand at 2283.738 kb on + strand at 2283.738 kb on + strand at 2283.738 kb on + strand at 2283.738 kb on + strand at 2283.738 kb on + strand at 2283.739 kb on - strand at 2283.739 kb on - strand at 2283.739 kb on - strand at 2283.739 kb on - strand at 2283.739 kb on - strand at 2283.739 kb on - strand at 2283.768 kb on + strand at 2283.807 kb on - strand at 2283.859 kb on + strand at 2283.859 kb on + strand at 2283.859 kb on + strand at 2283.860 kb on - strand at 2283.860 kb on - strand at 2283.860 kb on - strand at 2283.860 kb on - strand at 2283.861 kb on + strand at 2283.861 kb on + strand at 2283.861 kb on + strand at 2283.861 kb on + strand at 2283.861 kb on + strand at 2283.861 kb on + strand at 2283.861 kb on + strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.862 kb on - strand at 2283.895 kb on + strand at 2283.924 kb on + strand at 2283.924 kb on + strand at 2283.925 kb on - strand at 2283.925 kb on - strand at 2283.925 kb on - strand at 2284.032 kb on + strand at 2284.032 kb on + strand at 2284.032 kb on + strand at 2284.032 kb on + strand at 2284.032 kb on + strand at 2284.032 kb on + strand at 2284.032 kb on + strand at 2284.033 kb on - strand at 2284.033 kb on - strand at 2284.033 kb on - strand at 2284.033 kb on - strand at 2284.034 kb on + strand at 2284.271 kb on + strand, within Pf1N1B4_2002 at 2284.272 kb on - strand, within Pf1N1B4_2002 at 2284.272 kb on - strand, within Pf1N1B4_2002 at 2284.272 kb on - strand, within Pf1N1B4_2002 at 2284.560 kb on + strand, within Pf1N1B4_2002 at 2284.560 kb on + strand, within Pf1N1B4_2002 at 2284.560 kb on + strand, within Pf1N1B4_2002 at 2284.560 kb on + strand, within Pf1N1B4_2002 at 2284.560 kb on + strand, within Pf1N1B4_2002 at 2284.560 kb on + strand, within Pf1N1B4_2002 at 2284.561 kb on - strand, within Pf1N1B4_2002 at 2284.561 kb on - strand, within Pf1N1B4_2002 at 2284.561 kb on - strand, within Pf1N1B4_2002 at 2284.650 kb on + strand, within Pf1N1B4_2002 at 2284.650 kb on + strand, within Pf1N1B4_2002 at 2284.650 kb on + strand, within Pf1N1B4_2002 at 2284.650 kb on + strand, within Pf1N1B4_2002 at 2284.650 kb on + strand, within Pf1N1B4_2002 at 2284.650 kb on + strand, within Pf1N1B4_2002 at 2284.651 kb on - strand, within Pf1N1B4_2002 at 2284.651 kb on - strand, within Pf1N1B4_2002 at 2284.651 kb on - strand, within Pf1N1B4_2002 at 2284.651 kb on - strand, within Pf1N1B4_2002
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 2,282,347 + Pf1N1B4_2000 0.13 -0.8 2,282,368 + Pf1N1B4_2000 0.16 +0.8 2,282,368 + Pf1N1B4_2000 0.16 -0.8 2,282,368 + Pf1N1B4_2000 0.16 +3.3 2,282,368 + Pf1N1B4_2000 0.16 -1.1 2,282,368 + Pf1N1B4_2000 0.16 -0.1 2,282,369 - Pf1N1B4_2000 0.16 -0.9 2,282,369 - Pf1N1B4_2000 0.16 -1.3 2,282,369 - Pf1N1B4_2000 0.16 -1.1 2,282,376 - Pf1N1B4_2000 0.17 +0.9 2,282,376 - Pf1N1B4_2000 0.17 +0.5 2,282,427 + Pf1N1B4_2000 0.22 -0.0 2,282,427 + Pf1N1B4_2000 0.22 +0.9 2,282,427 + Pf1N1B4_2000 0.22 -0.5 2,282,428 - Pf1N1B4_2000 0.23 +0.1 2,282,428 - Pf1N1B4_2000 0.23 -0.8 2,282,559 + Pf1N1B4_2000 0.38 +1.5 2,282,559 + Pf1N1B4_2000 0.38 -1.2 2,282,561 + Pf1N1B4_2000 0.38 -0.3 2,282,561 + Pf1N1B4_2000 0.38 -0.1 2,282,561 + Pf1N1B4_2000 0.38 -0.2 2,282,561 + Pf1N1B4_2000 0.38 -1.5 2,282,561 + Pf1N1B4_2000 0.38 -1.5 2,282,561 + Pf1N1B4_2000 0.38 +0.5 2,282,561 + Pf1N1B4_2000 0.38 -0.1 2,282,562 - Pf1N1B4_2000 0.38 -1.3 2,282,562 - Pf1N1B4_2000 0.38 -1.5 2,282,562 - Pf1N1B4_2000 0.38 -0.1 2,282,562 - Pf1N1B4_2000 0.38 +0.4 2,282,562 - Pf1N1B4_2000 0.38 +0.5 2,282,793 - Pf1N1B4_2000 0.64 -2.6 2,283,023 + +0.4 2,283,023 + +1.1 2,283,023 + -1.5 2,283,023 + +0.1 2,283,023 + -1.5 2,283,023 + -1.1 2,283,024 - -1.9 2,283,024 - -0.2 2,283,159 + -1.8 2,283,160 - -2.2 2,283,160 - -0.8 2,283,221 + -1.3 2,283,221 + +0.2 2,283,221 + -1.8 2,283,221 + -1.6 2,283,222 - -0.8 2,283,222 - +1.1 2,283,222 - +1.7 2,283,222 - -0.6 2,283,222 - -1.5 2,283,247 + -0.3 2,283,247 + -0.8 2,283,247 + -0.5 2,283,247 + -1.0 2,283,248 - -0.5 2,283,327 + Pf1N1B4_2001 0.16 -0.8 2,283,327 + Pf1N1B4_2001 0.16 -2.4 2,283,328 - Pf1N1B4_2001 0.16 +0.5 2,283,328 - Pf1N1B4_2001 0.16 -1.1 2,283,435 - Pf1N1B4_2001 0.38 +0.3 2,283,501 + Pf1N1B4_2001 0.52 -0.3 2,283,501 + Pf1N1B4_2001 0.52 -1.9 2,283,501 + Pf1N1B4_2001 0.52 -1.3 2,283,501 + Pf1N1B4_2001 0.52 +0.9 2,283,502 - Pf1N1B4_2001 0.52 -1.6 2,283,502 - Pf1N1B4_2001 0.52 -1.5 2,283,502 - Pf1N1B4_2001 0.52 +2.1 2,283,588 + Pf1N1B4_2001 0.70 -0.6 2,283,589 - Pf1N1B4_2001 0.70 -0.3 2,283,589 - Pf1N1B4_2001 0.70 +0.5 2,283,618 + Pf1N1B4_2001 0.76 -1.5 2,283,636 + Pf1N1B4_2001 0.80 +0.9 2,283,738 + +0.1 2,283,738 + +0.5 2,283,738 + -0.1 2,283,738 + +1.1 2,283,738 + -0.4 2,283,738 + +0.2 2,283,738 + +1.5 2,283,739 - -2.3 2,283,739 - +0.9 2,283,739 - +0.4 2,283,739 - -1.8 2,283,739 - +1.4 2,283,739 - -0.3 2,283,768 + -0.3 2,283,807 - +0.1 2,283,859 + +0.7 2,283,859 + -1.5 2,283,859 + +0.2 2,283,860 - +0.5 2,283,860 - +0.7 2,283,860 - -0.3 2,283,860 - -1.3 2,283,861 + -1.3 2,283,861 + -1.6 2,283,861 + +1.5 2,283,861 + -0.5 2,283,861 + +1.1 2,283,861 + -0.5 2,283,861 + +0.5 2,283,862 - +0.2 2,283,862 - -0.3 2,283,862 - -0.5 2,283,862 - +0.5 2,283,862 - -1.3 2,283,862 - +0.4 2,283,862 - +0.7 2,283,862 - -2.1 2,283,862 - -0.8 2,283,862 - +0.3 2,283,862 - +0.5 2,283,862 - +0.9 2,283,862 - -1.8 2,283,862 - +1.5 2,283,862 - +1.2 2,283,862 - -0.8 2,283,862 - +0.5 2,283,862 - -0.1 2,283,895 + -1.1 2,283,924 + -0.8 2,283,924 + +0.5 2,283,925 - -0.5 2,283,925 - -0.5 2,283,925 - +0.5 2,284,032 + -0.1 2,284,032 + -0.8 2,284,032 + -2.5 2,284,032 + -0.5 2,284,032 + -2.1 2,284,032 + +0.3 2,284,032 + +1.2 2,284,033 - +1.8 2,284,033 - +1.2 2,284,033 - +1.0 2,284,033 - +0.6 2,284,034 + -0.5 2,284,271 + Pf1N1B4_2002 0.19 -0.8 2,284,272 - Pf1N1B4_2002 0.19 -0.8 2,284,272 - Pf1N1B4_2002 0.19 +0.4 2,284,272 - Pf1N1B4_2002 0.19 -0.5 2,284,560 + Pf1N1B4_2002 0.36 -1.3 2,284,560 + Pf1N1B4_2002 0.36 -1.1 2,284,560 + Pf1N1B4_2002 0.36 -0.8 2,284,560 + Pf1N1B4_2002 0.36 -1.8 2,284,560 + Pf1N1B4_2002 0.36 +1.0 2,284,560 + Pf1N1B4_2002 0.36 +0.5 2,284,561 - Pf1N1B4_2002 0.36 +0.8 2,284,561 - Pf1N1B4_2002 0.36 -0.2 2,284,561 - Pf1N1B4_2002 0.36 +0.3 2,284,650 + Pf1N1B4_2002 0.41 -0.1 2,284,650 + Pf1N1B4_2002 0.41 -0.5 2,284,650 + Pf1N1B4_2002 0.41 +0.2 2,284,650 + Pf1N1B4_2002 0.41 -0.5 2,284,650 + Pf1N1B4_2002 0.41 -0.4 2,284,650 + Pf1N1B4_2002 0.41 -0.1 2,284,651 - Pf1N1B4_2002 0.41 -0.3 2,284,651 - Pf1N1B4_2002 0.41 +0.6 2,284,651 - Pf1N1B4_2002 0.41 +0.2 2,284,651 - Pf1N1B4_2002 0.41 -1.0
Or see this region's nucleotide sequence