Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_192 and Pf1N1B4_193 are separated by 205 nucleotides Pf1N1B4_193 and Pf1N1B4_194 are separated by 101 nucleotides Pf1N1B4_194 and Pf1N1B4_195 are separated by 223 nucleotides
Pf1N1B4_192 - Cysteine desulfurase (EC 2.8.1.7), at 241,196 to 242,362
_192
Pf1N1B4_193 - Transporter, LysE family, at 242,568 to 243,182
_193
Pf1N1B4_194 - ABC-type proline/glycine betaine transport systems, ATPase components, at 243,284 to 243,553
_194
Pf1N1B4_195 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 243,777 to 244,235
_195
Position (kb)
242
243
244 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 241.710 kb on + strand, within Pf1N1B4_192 at 241.710 kb on + strand, within Pf1N1B4_192 at 241.710 kb on + strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.964 kb on - strand, within Pf1N1B4_192 at 241.964 kb on - strand, within Pf1N1B4_192 at 241.992 kb on + strand at 241.992 kb on + strand, within Pf1N1B4_192 at 241.992 kb on + strand at 241.993 kb on - strand at 241.993 kb on - strand, within Pf1N1B4_192 at 241.993 kb on - strand, within Pf1N1B4_192 at 241.993 kb on - strand at 241.993 kb on - strand, within Pf1N1B4_192 at 241.993 kb on - strand, within Pf1N1B4_192 at 242.344 kb on - strand at 242.344 kb on - strand at 242.363 kb on - strand at 242.462 kb on + strand at 242.462 kb on + strand at 242.463 kb on - strand at 242.501 kb on - strand at 242.510 kb on + strand at 242.534 kb on + strand at 242.534 kb on + strand at 242.761 kb on + strand, within Pf1N1B4_193 at 242.779 kb on + strand, within Pf1N1B4_193 at 242.851 kb on + strand, within Pf1N1B4_193 at 242.851 kb on + strand, within Pf1N1B4_193 at 242.852 kb on - strand, within Pf1N1B4_193 at 242.852 kb on - strand, within Pf1N1B4_193 at 242.932 kb on + strand, within Pf1N1B4_193 at 242.932 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.963 kb on - strand, within Pf1N1B4_193 at 242.963 kb on - strand, within Pf1N1B4_193 at 242.963 kb on - strand, within Pf1N1B4_193 at 242.963 kb on - strand, within Pf1N1B4_193 at 242.974 kb on + strand, within Pf1N1B4_193 at 242.974 kb on + strand, within Pf1N1B4_193 at 242.974 kb on + strand, within Pf1N1B4_193 at 242.975 kb on - strand, within Pf1N1B4_193 at 243.211 kb on + strand at 243.212 kb on - strand at 243.272 kb on + strand at 243.308 kb on + strand at 243.309 kb on - strand at 243.396 kb on + strand, within Pf1N1B4_194 at 243.465 kb on + strand, within Pf1N1B4_194 at 243.466 kb on - strand, within Pf1N1B4_194 at 243.466 kb on - strand, within Pf1N1B4_194 at 243.617 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.719 kb on + strand at 243.719 kb on + strand at 243.784 kb on + strand at 243.804 kb on + strand at 243.822 kb on + strand at 243.826 kb on + strand, within Pf1N1B4_195 at 243.826 kb on + strand, within Pf1N1B4_195 at 243.938 kb on + strand, within Pf1N1B4_195 at 243.938 kb on + strand, within Pf1N1B4_195 at 243.939 kb on - strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.055 kb on + strand, within Pf1N1B4_195 at 244.111 kb on - strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.154 kb on - strand, within Pf1N1B4_195 at 244.154 kb on - strand, within Pf1N1B4_195
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 241,710 + Pf1N1B4_192 0.44 -0.4 241,710 + Pf1N1B4_192 0.44 +1.0 241,710 + Pf1N1B4_192 0.44 -0.6 241,711 - Pf1N1B4_192 0.44 +0.7 241,711 - Pf1N1B4_192 0.44 -0.0 241,711 - Pf1N1B4_192 0.44 -1.5 241,711 - Pf1N1B4_192 0.44 +0.4 241,711 - Pf1N1B4_192 0.44 +0.1 241,964 - Pf1N1B4_192 0.66 -1.6 241,964 - Pf1N1B4_192 0.66 +1.4 241,992 + -0.6 241,992 + Pf1N1B4_192 0.68 -0.0 241,992 + -0.6 241,993 - -0.0 241,993 - Pf1N1B4_192 0.68 -0.5 241,993 - Pf1N1B4_192 0.68 +1.8 241,993 - +0.7 241,993 - Pf1N1B4_192 0.68 +0.7 241,993 - Pf1N1B4_192 0.68 -0.6 242,344 - +1.7 242,344 - -0.6 242,363 - -0.9 242,462 + +1.5 242,462 + -0.5 242,463 - -0.1 242,501 - +0.5 242,510 + +1.0 242,534 + +0.7 242,534 + -1.0 242,761 + Pf1N1B4_193 0.31 +1.0 242,779 + Pf1N1B4_193 0.34 +1.4 242,851 + Pf1N1B4_193 0.46 -1.4 242,851 + Pf1N1B4_193 0.46 -2.2 242,852 - Pf1N1B4_193 0.46 -0.0 242,852 - Pf1N1B4_193 0.46 -1.4 242,932 + Pf1N1B4_193 0.59 -0.6 242,932 + Pf1N1B4_193 0.59 -1.0 242,962 + Pf1N1B4_193 0.64 -1.0 242,962 + Pf1N1B4_193 0.64 -0.0 242,962 + Pf1N1B4_193 0.64 -1.8 242,962 + Pf1N1B4_193 0.64 -1.4 242,962 + Pf1N1B4_193 0.64 -1.4 242,963 - Pf1N1B4_193 0.64 +0.4 242,963 - Pf1N1B4_193 0.64 +1.2 242,963 - Pf1N1B4_193 0.64 -1.4 242,963 - Pf1N1B4_193 0.64 +1.0 242,974 + Pf1N1B4_193 0.66 -1.4 242,974 + Pf1N1B4_193 0.66 -1.4 242,974 + Pf1N1B4_193 0.66 +0.4 242,975 - Pf1N1B4_193 0.66 +1.5 243,211 + +1.3 243,212 - -2.4 243,272 + +0.1 243,308 + -1.3 243,309 - -1.6 243,396 + Pf1N1B4_194 0.41 +0.4 243,465 + Pf1N1B4_194 0.67 +0.4 243,466 - Pf1N1B4_194 0.67 +1.0 243,466 - Pf1N1B4_194 0.67 -0.8 243,617 + +1.4 243,713 + -0.1 243,713 + -0.0 243,713 + -0.6 243,713 + +1.0 243,713 + +0.3 243,713 + -1.0 243,713 + +0.0 243,715 + -1.0 243,715 + -1.4 243,715 + +1.0 243,715 + -0.5 243,715 + -1.0 243,719 + -1.4 243,719 + -1.0 243,784 + +1.1 243,804 + -2.5 243,822 + -1.8 243,826 + Pf1N1B4_195 0.11 +0.3 243,826 + Pf1N1B4_195 0.11 +0.7 243,938 + Pf1N1B4_195 0.35 -1.6 243,938 + Pf1N1B4_195 0.35 -0.4 243,939 - Pf1N1B4_195 0.35 +0.7 244,029 + Pf1N1B4_195 0.55 -0.6 244,029 + Pf1N1B4_195 0.55 -0.3 244,029 + Pf1N1B4_195 0.55 -2.0 244,029 + Pf1N1B4_195 0.55 +0.6 244,030 - Pf1N1B4_195 0.55 +0.2 244,030 - Pf1N1B4_195 0.55 +1.0 244,030 - Pf1N1B4_195 0.55 -2.2 244,030 - Pf1N1B4_195 0.55 -1.0 244,055 + Pf1N1B4_195 0.61 +0.4 244,111 - Pf1N1B4_195 0.73 +0.6 244,153 + Pf1N1B4_195 0.82 -1.3 244,153 + +2.0 244,153 + Pf1N1B4_195 0.82 -1.4 244,153 + -1.0 244,153 + Pf1N1B4_195 0.82 -1.0 244,153 + Pf1N1B4_195 0.82 +0.3 244,153 + Pf1N1B4_195 0.82 +0.8 244,154 - Pf1N1B4_195 0.82 +0.5 244,154 - Pf1N1B4_195 0.82 +0.5
Or see this region's nucleotide sequence