Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_174 and Pf1N1B4_175 overlap by 1 nucleotides Pf1N1B4_175 and Pf1N1B4_176 are separated by 247 nucleotides
Pf1N1B4_174 - Lipid A export ATP-binding/permease protein MsbA, at 215,454 to 217,172
_174
Pf1N1B4_175 - sigma-54 dependent transcriptional regulator, at 217,172 to 219,076
_175
Pf1N1B4_176 - Acetylpolyamine aminohydrolase, at 219,324 to 220,349
_176
Position (kb) 217
218
219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 216.236 kb on - strand, within Pf1N1B4_174 at 216.281 kb on - strand, within Pf1N1B4_174 at 216.439 kb on + strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.802 kb on + strand, within Pf1N1B4_174 at 217.100 kb on - strand at 217.190 kb on - strand at 218.173 kb on - strand, within Pf1N1B4_175 at 218.388 kb on + strand, within Pf1N1B4_175 at 218.563 kb on - strand at 218.608 kb on - strand, within Pf1N1B4_175 at 218.608 kb on - strand, within Pf1N1B4_175 at 218.981 kb on + strand at 218.981 kb on + strand at 219.044 kb on + strand at 219.044 kb on + strand at 219.045 kb on - strand at 219.045 kb on - strand at 219.045 kb on - strand at 219.069 kb on - strand at 219.169 kb on - strand at 219.169 kb on - strand at 219.306 kb on + strand at 219.307 kb on - strand at 219.507 kb on - strand, within Pf1N1B4_176 at 219.718 kb on + strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.848 kb on - strand, within Pf1N1B4_176 at 219.908 kb on - strand, within Pf1N1B4_176 at 220.021 kb on + strand, within Pf1N1B4_176 at 220.022 kb on - strand, within Pf1N1B4_176
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 216,236 - Pf1N1B4_174 0.45 -0.6 216,281 - Pf1N1B4_174 0.48 -0.5 216,439 + Pf1N1B4_174 0.57 -0.6 216,440 - Pf1N1B4_174 0.57 -1.4 216,440 - Pf1N1B4_174 0.57 -1.3 216,440 - Pf1N1B4_174 0.57 -0.6 216,440 - Pf1N1B4_174 0.57 -0.6 216,440 - Pf1N1B4_174 0.57 -0.6 216,802 + Pf1N1B4_174 0.78 +0.4 217,100 - -1.8 217,190 - -1.4 218,173 - Pf1N1B4_175 0.53 -0.6 218,388 + Pf1N1B4_175 0.64 -3.0 218,563 - +2.0 218,608 - Pf1N1B4_175 0.75 -1.4 218,608 - Pf1N1B4_175 0.75 -1.0 218,981 + -0.2 218,981 + +0.1 219,044 + +0.4 219,044 + +0.8 219,045 - -0.6 219,045 - +0.4 219,045 - -0.9 219,069 - +0.6 219,169 - -0.6 219,169 - -0.4 219,306 + +3.0 219,307 - -2.4 219,507 - Pf1N1B4_176 0.18 +0.7 219,718 + Pf1N1B4_176 0.38 +0.7 219,719 - Pf1N1B4_176 0.38 -1.0 219,719 - Pf1N1B4_176 0.38 +1.3 219,719 - Pf1N1B4_176 0.38 +1.0 219,847 + Pf1N1B4_176 0.51 -0.6 219,847 + Pf1N1B4_176 0.51 +1.0 219,848 - Pf1N1B4_176 0.51 -0.6 219,908 - Pf1N1B4_176 0.57 -0.0 220,021 + Pf1N1B4_176 0.68 +1.4 220,022 - Pf1N1B4_176 0.68 -0.4
Or see this region's nucleotide sequence