Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1736 and Pf1N1B4_1737 are separated by 81 nucleotides Pf1N1B4_1737 and Pf1N1B4_1738 overlap by 4 nucleotides Pf1N1B4_1738 and Pf1N1B4_1739 overlap by 4 nucleotides
Pf1N1B4_1736 - hypothetical protein, at 1,985,781 to 1,985,987
_1736
Pf1N1B4_1737 - hypothetical protein, at 1,986,069 to 1,986,707
_1737
Pf1N1B4_1738 - RNA polymerase sigma-54 factor RpoN, at 1,986,704 to 1,987,306
_1738
Pf1N1B4_1739 - Von Willebrand factor type A domain protein, at 1,987,303 to 1,989,045
_1739
Position (kb)
1986
1987
1988 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1985.711 kb on - strand at 1985.711 kb on - strand at 1985.721 kb on + strand at 1985.722 kb on - strand at 1985.722 kb on - strand at 1985.722 kb on - strand at 1985.722 kb on - strand at 1985.748 kb on + strand at 1985.749 kb on - strand at 1985.749 kb on - strand at 1985.749 kb on - strand at 1985.749 kb on - strand at 1985.749 kb on - strand at 1985.809 kb on + strand, within Pf1N1B4_1736 at 1985.810 kb on - strand, within Pf1N1B4_1736 at 1986.012 kb on - strand at 1986.714 kb on - strand at 1986.714 kb on - strand at 1986.714 kb on - strand at 1986.914 kb on + strand, within Pf1N1B4_1738 at 1986.915 kb on - strand, within Pf1N1B4_1738 at 1986.915 kb on - strand, within Pf1N1B4_1738 at 1986.915 kb on - strand, within Pf1N1B4_1738 at 1986.969 kb on + strand at 1986.969 kb on + strand, within Pf1N1B4_1738 at 1986.969 kb on + strand, within Pf1N1B4_1738 at 1986.969 kb on + strand, within Pf1N1B4_1738 at 1986.969 kb on + strand, within Pf1N1B4_1738 at 1986.969 kb on + strand, within Pf1N1B4_1738 at 1986.969 kb on + strand, within Pf1N1B4_1738 at 1986.970 kb on - strand, within Pf1N1B4_1738 at 1986.970 kb on - strand, within Pf1N1B4_1738 at 1986.970 kb on - strand, within Pf1N1B4_1738 at 1986.970 kb on - strand, within Pf1N1B4_1738 at 1986.970 kb on - strand, within Pf1N1B4_1738 at 1986.970 kb on - strand, within Pf1N1B4_1738 at 1987.016 kb on + strand, within Pf1N1B4_1738 at 1987.016 kb on + strand, within Pf1N1B4_1738 at 1987.016 kb on + strand, within Pf1N1B4_1738 at 1987.016 kb on + strand, within Pf1N1B4_1738 at 1987.017 kb on - strand at 1987.017 kb on - strand, within Pf1N1B4_1738 at 1987.017 kb on - strand, within Pf1N1B4_1738 at 1987.042 kb on - strand, within Pf1N1B4_1738 at 1987.042 kb on - strand, within Pf1N1B4_1738 at 1987.116 kb on + strand, within Pf1N1B4_1738 at 1987.167 kb on + strand, within Pf1N1B4_1738 at 1987.272 kb on - strand at 1987.310 kb on - strand at 1987.469 kb on + strand at 1987.591 kb on - strand, within Pf1N1B4_1739 at 1987.591 kb on - strand at 1987.724 kb on + strand, within Pf1N1B4_1739 at 1987.724 kb on + strand, within Pf1N1B4_1739 at 1987.725 kb on - strand, within Pf1N1B4_1739 at 1987.725 kb on - strand, within Pf1N1B4_1739 at 1987.725 kb on - strand, within Pf1N1B4_1739 at 1987.725 kb on - strand, within Pf1N1B4_1739 at 1987.725 kb on - strand, within Pf1N1B4_1739 at 1987.740 kb on + strand, within Pf1N1B4_1739 at 1987.740 kb on + strand, within Pf1N1B4_1739 at 1987.740 kb on + strand, within Pf1N1B4_1739 at 1987.740 kb on + strand, within Pf1N1B4_1739 at 1987.740 kb on + strand, within Pf1N1B4_1739 at 1987.740 kb on + strand, within Pf1N1B4_1739 at 1987.741 kb on - strand, within Pf1N1B4_1739 at 1987.741 kb on - strand, within Pf1N1B4_1739 at 1987.741 kb on - strand, within Pf1N1B4_1739 at 1987.741 kb on - strand, within Pf1N1B4_1739 at 1987.741 kb on - strand, within Pf1N1B4_1739 at 1987.741 kb on - strand, within Pf1N1B4_1739 at 1987.741 kb on - strand, within Pf1N1B4_1739 at 1987.741 kb on - strand at 1987.741 kb on - strand, within Pf1N1B4_1739 at 1987.768 kb on + strand, within Pf1N1B4_1739 at 1987.768 kb on + strand, within Pf1N1B4_1739 at 1987.768 kb on + strand, within Pf1N1B4_1739 at 1987.768 kb on + strand, within Pf1N1B4_1739 at 1987.769 kb on - strand, within Pf1N1B4_1739 at 1987.769 kb on - strand, within Pf1N1B4_1739 at 1987.949 kb on + strand, within Pf1N1B4_1739 at 1987.950 kb on - strand, within Pf1N1B4_1739 at 1987.950 kb on - strand, within Pf1N1B4_1739 at 1987.950 kb on - strand, within Pf1N1B4_1739 at 1987.955 kb on + strand, within Pf1N1B4_1739 at 1987.955 kb on + strand, within Pf1N1B4_1739 at 1987.955 kb on + strand, within Pf1N1B4_1739 at 1987.956 kb on - strand, within Pf1N1B4_1739 at 1987.956 kb on - strand, within Pf1N1B4_1739 at 1987.956 kb on - strand, within Pf1N1B4_1739 at 1987.956 kb on - strand, within Pf1N1B4_1739 at 1987.956 kb on - strand, within Pf1N1B4_1739 at 1987.956 kb on - strand, within Pf1N1B4_1739 at 1987.959 kb on + strand, within Pf1N1B4_1739 at 1987.959 kb on + strand, within Pf1N1B4_1739 at 1988.003 kb on + strand, within Pf1N1B4_1739 at 1988.003 kb on + strand at 1988.003 kb on + strand, within Pf1N1B4_1739 at 1988.003 kb on + strand, within Pf1N1B4_1739 at 1988.004 kb on - strand, within Pf1N1B4_1739 at 1988.004 kb on - strand, within Pf1N1B4_1739 at 1988.004 kb on - strand, within Pf1N1B4_1739 at 1988.004 kb on - strand, within Pf1N1B4_1739 at 1988.004 kb on - strand, within Pf1N1B4_1739 at 1988.004 kb on - strand, within Pf1N1B4_1739 at 1988.004 kb on - strand, within Pf1N1B4_1739 at 1988.004 kb on - strand, within Pf1N1B4_1739 at 1988.004 kb on - strand, within Pf1N1B4_1739 at 1988.008 kb on - strand, within Pf1N1B4_1739 at 1988.300 kb on + strand, within Pf1N1B4_1739 at 1988.300 kb on + strand, within Pf1N1B4_1739 at 1988.300 kb on + strand, within Pf1N1B4_1739 at 1988.301 kb on - strand, within Pf1N1B4_1739
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 1,985,711 - +0.2 1,985,711 - +0.7 1,985,721 + +1.7 1,985,722 - -0.1 1,985,722 - -1.1 1,985,722 - +0.5 1,985,722 - +0.1 1,985,748 + -0.5 1,985,749 - +0.2 1,985,749 - +0.0 1,985,749 - -0.8 1,985,749 - +0.2 1,985,749 - +0.5 1,985,809 + Pf1N1B4_1736 0.14 -1.8 1,985,810 - Pf1N1B4_1736 0.14 -0.7 1,986,012 - -0.3 1,986,714 - +0.3 1,986,714 - +0.3 1,986,714 - +1.5 1,986,914 + Pf1N1B4_1738 0.35 +1.1 1,986,915 - Pf1N1B4_1738 0.35 -1.3 1,986,915 - Pf1N1B4_1738 0.35 -1.5 1,986,915 - Pf1N1B4_1738 0.35 -0.3 1,986,969 + +0.9 1,986,969 + Pf1N1B4_1738 0.44 -2.1 1,986,969 + Pf1N1B4_1738 0.44 +0.5 1,986,969 + Pf1N1B4_1738 0.44 -0.5 1,986,969 + Pf1N1B4_1738 0.44 -0.8 1,986,969 + Pf1N1B4_1738 0.44 -0.3 1,986,969 + Pf1N1B4_1738 0.44 -0.5 1,986,970 - Pf1N1B4_1738 0.44 -2.7 1,986,970 - Pf1N1B4_1738 0.44 -1.1 1,986,970 - Pf1N1B4_1738 0.44 -1.1 1,986,970 - Pf1N1B4_1738 0.44 -1.3 1,986,970 - Pf1N1B4_1738 0.44 +2.1 1,986,970 - Pf1N1B4_1738 0.44 +0.2 1,987,016 + Pf1N1B4_1738 0.52 -2.0 1,987,016 + Pf1N1B4_1738 0.52 -1.4 1,987,016 + Pf1N1B4_1738 0.52 -1.3 1,987,016 + Pf1N1B4_1738 0.52 -1.5 1,987,017 - +0.5 1,987,017 - Pf1N1B4_1738 0.52 -0.8 1,987,017 - Pf1N1B4_1738 0.52 -0.5 1,987,042 - Pf1N1B4_1738 0.56 -0.8 1,987,042 - Pf1N1B4_1738 0.56 -0.7 1,987,116 + Pf1N1B4_1738 0.68 +0.9 1,987,167 + Pf1N1B4_1738 0.77 -1.3 1,987,272 - +0.5 1,987,310 - -0.3 1,987,469 + -0.9 1,987,591 - Pf1N1B4_1739 0.17 -0.5 1,987,591 - +0.5 1,987,724 + Pf1N1B4_1739 0.24 -1.1 1,987,724 + Pf1N1B4_1739 0.24 +0.7 1,987,725 - Pf1N1B4_1739 0.24 +0.4 1,987,725 - Pf1N1B4_1739 0.24 +0.4 1,987,725 - Pf1N1B4_1739 0.24 +1.8 1,987,725 - Pf1N1B4_1739 0.24 -0.2 1,987,725 - Pf1N1B4_1739 0.24 -2.2 1,987,740 + Pf1N1B4_1739 0.25 -1.1 1,987,740 + Pf1N1B4_1739 0.25 +1.1 1,987,740 + Pf1N1B4_1739 0.25 +1.9 1,987,740 + Pf1N1B4_1739 0.25 +0.6 1,987,740 + Pf1N1B4_1739 0.25 -1.5 1,987,740 + Pf1N1B4_1739 0.25 +1.5 1,987,741 - Pf1N1B4_1739 0.25 -0.7 1,987,741 - Pf1N1B4_1739 0.25 -1.6 1,987,741 - Pf1N1B4_1739 0.25 -0.3 1,987,741 - Pf1N1B4_1739 0.25 +0.6 1,987,741 - Pf1N1B4_1739 0.25 +1.5 1,987,741 - Pf1N1B4_1739 0.25 -1.5 1,987,741 - Pf1N1B4_1739 0.25 -1.5 1,987,741 - -0.5 1,987,741 - Pf1N1B4_1739 0.25 -1.3 1,987,768 + Pf1N1B4_1739 0.27 -0.5 1,987,768 + Pf1N1B4_1739 0.27 +1.2 1,987,768 + Pf1N1B4_1739 0.27 -1.8 1,987,768 + Pf1N1B4_1739 0.27 -1.2 1,987,769 - Pf1N1B4_1739 0.27 -1.7 1,987,769 - Pf1N1B4_1739 0.27 -0.7 1,987,949 + Pf1N1B4_1739 0.37 -0.1 1,987,950 - Pf1N1B4_1739 0.37 -0.8 1,987,950 - Pf1N1B4_1739 0.37 +1.3 1,987,950 - Pf1N1B4_1739 0.37 +0.5 1,987,955 + Pf1N1B4_1739 0.37 +1.8 1,987,955 + Pf1N1B4_1739 0.37 -1.7 1,987,955 + Pf1N1B4_1739 0.37 -1.0 1,987,956 - Pf1N1B4_1739 0.37 +1.8 1,987,956 - Pf1N1B4_1739 0.37 -0.5 1,987,956 - Pf1N1B4_1739 0.37 -0.1 1,987,956 - Pf1N1B4_1739 0.37 +0.9 1,987,956 - Pf1N1B4_1739 0.37 +0.0 1,987,956 - Pf1N1B4_1739 0.37 +0.2 1,987,959 + Pf1N1B4_1739 0.38 -0.8 1,987,959 + Pf1N1B4_1739 0.38 -0.7 1,988,003 + Pf1N1B4_1739 0.40 +0.2 1,988,003 + +0.5 1,988,003 + Pf1N1B4_1739 0.40 -0.7 1,988,003 + Pf1N1B4_1739 0.40 -1.1 1,988,004 - Pf1N1B4_1739 0.40 -1.5 1,988,004 - Pf1N1B4_1739 0.40 -0.8 1,988,004 - Pf1N1B4_1739 0.40 +0.6 1,988,004 - Pf1N1B4_1739 0.40 +0.8 1,988,004 - Pf1N1B4_1739 0.40 -0.8 1,988,004 - Pf1N1B4_1739 0.40 +0.4 1,988,004 - Pf1N1B4_1739 0.40 +0.8 1,988,004 - Pf1N1B4_1739 0.40 +0.6 1,988,004 - Pf1N1B4_1739 0.40 -1.5 1,988,008 - Pf1N1B4_1739 0.40 +0.1 1,988,300 + Pf1N1B4_1739 0.57 -1.1 1,988,300 + Pf1N1B4_1739 0.57 -0.3 1,988,300 + Pf1N1B4_1739 0.57 -0.1 1,988,301 - Pf1N1B4_1739 0.57 +0.2
Or see this region's nucleotide sequence