Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1569 and Pf1N1B4_1570 overlap by 4 nucleotides Pf1N1B4_1570 and Pf1N1B4_1571 are separated by 219 nucleotides Pf1N1B4_1571 and Pf1N1B4_1572 are separated by 144 nucleotides
Pf1N1B4_1569 - Twin-arginine translocation protein TatC, at 1,805,133 to 1,805,927
_1569
Pf1N1B4_1570 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 1,805,924 to 1,806,631
_1570
Pf1N1B4_1571 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 1,806,851 to 1,808,767
_1571
Pf1N1B4_1572 - Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-), at 1,808,912 to 1,811,482
_1572
Position (kb)
1806
1807
1808
1809 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1806.081 kb on + strand, within Pf1N1B4_1570 at 1806.081 kb on + strand, within Pf1N1B4_1570 at 1806.082 kb on - strand, within Pf1N1B4_1570 at 1806.082 kb on - strand at 1806.154 kb on - strand, within Pf1N1B4_1570 at 1806.452 kb on + strand, within Pf1N1B4_1570 at 1806.452 kb on + strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.645 kb on - strand at 1806.717 kb on + strand at 1806.775 kb on + strand at 1806.784 kb on - strand at 1806.784 kb on - strand at 1806.818 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.829 kb on - strand at 1806.844 kb on + strand at 1806.844 kb on + strand at 1806.845 kb on - strand at 1806.854 kb on + strand at 1806.854 kb on + strand at 1806.854 kb on + strand at 1806.854 kb on + strand at 1806.855 kb on - strand at 1806.885 kb on + strand at 1806.885 kb on + strand at 1806.885 kb on + strand at 1806.885 kb on + strand at 1806.886 kb on - strand at 1806.886 kb on - strand at 1806.886 kb on - strand at 1806.886 kb on - strand at 1807.007 kb on - strand at 1807.058 kb on + strand, within Pf1N1B4_1571 at 1807.059 kb on - strand, within Pf1N1B4_1571 at 1807.206 kb on - strand, within Pf1N1B4_1571 at 1807.225 kb on + strand, within Pf1N1B4_1571 at 1807.226 kb on - strand, within Pf1N1B4_1571 at 1807.226 kb on - strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.416 kb on - strand at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.418 kb on - strand, within Pf1N1B4_1571 at 1807.680 kb on + strand, within Pf1N1B4_1571 at 1807.681 kb on - strand, within Pf1N1B4_1571 at 1809.270 kb on + strand, within Pf1N1B4_1572 at 1809.271 kb on - strand, within Pf1N1B4_1572 at 1809.306 kb on + strand, within Pf1N1B4_1572 at 1809.306 kb on + strand, within Pf1N1B4_1572 at 1809.307 kb on - strand, within Pf1N1B4_1572
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 1,806,081 + Pf1N1B4_1570 0.22 -1.8 1,806,081 + Pf1N1B4_1570 0.22 +0.0 1,806,082 - Pf1N1B4_1570 0.22 +0.8 1,806,082 - -0.9 1,806,154 - Pf1N1B4_1570 0.32 +0.5 1,806,452 + Pf1N1B4_1570 0.75 -0.4 1,806,452 + Pf1N1B4_1570 0.75 +0.1 1,806,453 - Pf1N1B4_1570 0.75 -0.9 1,806,453 - Pf1N1B4_1570 0.75 +1.1 1,806,453 - Pf1N1B4_1570 0.75 -0.2 1,806,453 - Pf1N1B4_1570 0.75 -1.2 1,806,453 - Pf1N1B4_1570 0.75 -1.8 1,806,453 - Pf1N1B4_1570 0.75 -0.4 1,806,645 - -1.9 1,806,717 + -0.0 1,806,775 + +1.4 1,806,784 - -0.6 1,806,784 - +1.4 1,806,818 + -2.2 1,806,828 + +2.0 1,806,828 + -0.9 1,806,828 + -0.6 1,806,828 + -0.6 1,806,828 + -0.6 1,806,829 - +0.1 1,806,844 + +0.4 1,806,844 + +1.4 1,806,845 - -0.0 1,806,854 + -0.0 1,806,854 + -0.2 1,806,854 + +0.2 1,806,854 + -1.2 1,806,855 - -1.2 1,806,885 + -0.9 1,806,885 + -1.2 1,806,885 + -0.0 1,806,885 + +1.4 1,806,886 - -2.7 1,806,886 - -0.8 1,806,886 - -0.3 1,806,886 - +0.8 1,807,007 - -0.4 1,807,058 + Pf1N1B4_1571 0.11 +1.4 1,807,059 - Pf1N1B4_1571 0.11 -3.0 1,807,206 - Pf1N1B4_1571 0.19 +1.6 1,807,225 + Pf1N1B4_1571 0.20 -1.2 1,807,226 - Pf1N1B4_1571 0.20 -2.2 1,807,226 - Pf1N1B4_1571 0.20 -1.8 1,807,415 + Pf1N1B4_1571 0.29 -1.4 1,807,415 + Pf1N1B4_1571 0.29 +0.4 1,807,415 + Pf1N1B4_1571 0.29 -0.9 1,807,415 + Pf1N1B4_1571 0.29 -2.3 1,807,415 + Pf1N1B4_1571 0.29 -0.6 1,807,415 + Pf1N1B4_1571 0.29 -2.3 1,807,416 - +2.4 1,807,416 - Pf1N1B4_1571 0.29 -1.0 1,807,416 - Pf1N1B4_1571 0.29 +0.4 1,807,416 - Pf1N1B4_1571 0.29 +0.6 1,807,416 - Pf1N1B4_1571 0.29 -0.6 1,807,416 - -0.6 1,807,416 - Pf1N1B4_1571 0.29 -1.4 1,807,416 - Pf1N1B4_1571 0.29 -0.4 1,807,416 - Pf1N1B4_1571 0.29 +0.1 1,807,416 - Pf1N1B4_1571 0.29 -1.4 1,807,418 - Pf1N1B4_1571 0.30 +0.8 1,807,680 + Pf1N1B4_1571 0.43 -0.2 1,807,681 - Pf1N1B4_1571 0.43 -1.9 1,809,270 + Pf1N1B4_1572 0.14 +2.1 1,809,271 - Pf1N1B4_1572 0.14 +1.2 1,809,306 + Pf1N1B4_1572 0.15 +0.4 1,809,306 + Pf1N1B4_1572 0.15 -1.6 1,809,307 - Pf1N1B4_1572 0.15 -2.2
Or see this region's nucleotide sequence