Experiment: D-Glucosamine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1311 and Pf1N1B4_1312 are separated by 83 nucleotides Pf1N1B4_1312 and Pf1N1B4_1313 overlap by 36 nucleotides Pf1N1B4_1313 and Pf1N1B4_1314 are separated by 38 nucleotides Pf1N1B4_1314 and Pf1N1B4_1315 are separated by 82 nucleotides
Pf1N1B4_1311 - Probable transmembrane protein, at 1,494,621 to 1,495,841
_1311
Pf1N1B4_1312 - hypothetical protein, at 1,495,925 to 1,496,062
_1312
Pf1N1B4_1313 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 1,496,027 to 1,497,037
_1313
Pf1N1B4_1314 - DNA-binding protein Fis, at 1,497,076 to 1,497,354
_1314
Pf1N1B4_1315 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 1,497,437 to 1,499,044
_1315
Position (kb)
1496
1497
1498 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1495.303 kb on + strand, within Pf1N1B4_1311 at 1495.304 kb on - strand, within Pf1N1B4_1311 at 1495.426 kb on - strand, within Pf1N1B4_1311 at 1495.435 kb on - strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.611 kb on + strand, within Pf1N1B4_1311 at 1495.612 kb on - strand, within Pf1N1B4_1311 at 1495.612 kb on - strand, within Pf1N1B4_1311 at 1495.612 kb on - strand, within Pf1N1B4_1311 at 1495.713 kb on + strand, within Pf1N1B4_1311 at 1495.812 kb on + strand at 1495.812 kb on + strand at 1495.812 kb on + strand at 1495.871 kb on + strand at 1495.890 kb on + strand at 1495.893 kb on + strand at 1495.893 kb on + strand at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.026 kb on + strand, within Pf1N1B4_1312 at 1496.051 kb on + strand at 1496.051 kb on + strand at 1496.051 kb on + strand at 1496.051 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.053 kb on + strand at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.145 kb on + strand, within Pf1N1B4_1313 at 1496.320 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.582 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.843 kb on + strand, within Pf1N1B4_1313 at 1496.843 kb on + strand, within Pf1N1B4_1313 at 1496.868 kb on + strand, within Pf1N1B4_1313 at 1496.868 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.996 kb on + strand at 1496.996 kb on + strand at 1496.996 kb on + strand at 1496.996 kb on + strand at 1497.056 kb on + strand at 1497.352 kb on + strand at 1497.615 kb on - strand, within Pf1N1B4_1315 at 1497.903 kb on - strand, within Pf1N1B4_1315 at 1497.944 kb on + strand, within Pf1N1B4_1315 at 1497.944 kb on + strand, within Pf1N1B4_1315 at 1497.944 kb on + strand, within Pf1N1B4_1315 at 1497.945 kb on - strand, within Pf1N1B4_1315 at 1497.945 kb on - strand, within Pf1N1B4_1315 at 1497.945 kb on - strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (N) remove 1,495,303 + Pf1N1B4_1311 0.56 -1.8 1,495,304 - Pf1N1B4_1311 0.56 -2.0 1,495,426 - Pf1N1B4_1311 0.66 +0.2 1,495,435 - Pf1N1B4_1311 0.67 +1.0 1,495,611 + Pf1N1B4_1311 0.81 +1.7 1,495,611 + Pf1N1B4_1311 0.81 +1.9 1,495,611 + Pf1N1B4_1311 0.81 -0.7 1,495,611 + Pf1N1B4_1311 0.81 +0.1 1,495,611 + Pf1N1B4_1311 0.81 -1.0 1,495,612 - Pf1N1B4_1311 0.81 +0.9 1,495,612 - Pf1N1B4_1311 0.81 -1.1 1,495,612 - Pf1N1B4_1311 0.81 +1.0 1,495,713 + Pf1N1B4_1311 0.89 -1.5 1,495,812 + -0.2 1,495,812 + +0.3 1,495,812 + -1.0 1,495,871 + -1.5 1,495,890 + -0.6 1,495,893 + +0.2 1,495,893 + +0.9 1,496,026 + Pf1N1B4_1312 0.73 +0.3 1,496,026 + Pf1N1B4_1312 0.73 -0.4 1,496,026 + Pf1N1B4_1312 0.73 +1.1 1,496,026 + Pf1N1B4_1312 0.73 -1.5 1,496,026 + Pf1N1B4_1312 0.73 -0.5 1,496,026 + Pf1N1B4_1312 0.73 +0.8 1,496,026 + Pf1N1B4_1312 0.73 -0.2 1,496,026 + Pf1N1B4_1312 0.73 +3.5 1,496,051 + -0.2 1,496,051 + +2.0 1,496,051 + +0.6 1,496,051 + +0.5 1,496,053 + -0.1 1,496,053 + +0.9 1,496,053 + -0.2 1,496,053 + +2.9 1,496,053 + +0.2 1,496,053 + +0.3 1,496,053 + +0.3 1,496,053 + -1.8 1,496,053 + +0.8 1,496,053 + +1.3 1,496,053 + +0.3 1,496,053 + +2.1 1,496,053 + +1.1 1,496,053 + -1.0 1,496,053 + +0.2 1,496,053 + -0.4 1,496,053 + +0.1 1,496,053 + +1.3 1,496,053 + +0.3 1,496,053 + -0.7 1,496,053 + +2.2 1,496,053 + +2.7 1,496,053 + +0.6 1,496,053 + -0.5 1,496,145 + Pf1N1B4_1313 0.12 -1.5 1,496,145 + Pf1N1B4_1313 0.12 -0.5 1,496,145 + Pf1N1B4_1313 0.12 +2.1 1,496,145 + Pf1N1B4_1313 0.12 +0.6 1,496,145 + Pf1N1B4_1313 0.12 +1.9 1,496,320 + Pf1N1B4_1313 0.29 +0.5 1,496,564 + Pf1N1B4_1313 0.53 +0.3 1,496,564 + Pf1N1B4_1313 0.53 +3.7 1,496,564 + Pf1N1B4_1313 0.53 +1.3 1,496,564 + Pf1N1B4_1313 0.53 +0.9 1,496,564 + Pf1N1B4_1313 0.53 +1.1 1,496,564 + Pf1N1B4_1313 0.53 -0.8 1,496,564 + Pf1N1B4_1313 0.53 -0.4 1,496,564 + Pf1N1B4_1313 0.53 +1.7 1,496,582 + Pf1N1B4_1313 0.55 +1.7 1,496,753 + Pf1N1B4_1313 0.72 +0.2 1,496,753 + Pf1N1B4_1313 0.72 +1.9 1,496,753 + Pf1N1B4_1313 0.72 +1.8 1,496,843 + Pf1N1B4_1313 0.81 +2.3 1,496,843 + Pf1N1B4_1313 0.81 +1.9 1,496,868 + Pf1N1B4_1313 0.83 +0.9 1,496,868 + Pf1N1B4_1313 0.83 +1.0 1,496,891 + Pf1N1B4_1313 0.85 +0.3 1,496,891 + Pf1N1B4_1313 0.85 +0.9 1,496,891 + Pf1N1B4_1313 0.85 -0.5 1,496,891 + Pf1N1B4_1313 0.85 +1.4 1,496,969 + -0.4 1,496,969 + -0.7 1,496,969 + +0.8 1,496,969 + +0.8 1,496,969 + +0.5 1,496,996 + -1.1 1,496,996 + +0.6 1,496,996 + +0.3 1,496,996 + -1.8 1,497,056 + +0.8 1,497,352 + -0.7 1,497,615 - Pf1N1B4_1315 0.11 -1.1 1,497,903 - Pf1N1B4_1315 0.29 -0.5 1,497,944 + Pf1N1B4_1315 0.32 -2.8 1,497,944 + Pf1N1B4_1315 0.32 -2.2 1,497,944 + Pf1N1B4_1315 0.32 -0.9 1,497,945 - Pf1N1B4_1315 0.32 -0.1 1,497,945 - Pf1N1B4_1315 0.32 -3.9 1,497,945 - Pf1N1B4_1315 0.32 +3.3 1,498,016 + Pf1N1B4_1315 0.36 -2.4 1,498,016 + Pf1N1B4_1315 0.36 -1.5 1,498,016 + Pf1N1B4_1315 0.36 -1.7 1,498,016 + Pf1N1B4_1315 0.36 +0.3 1,498,016 + Pf1N1B4_1315 0.36 +1.3 1,498,016 + Pf1N1B4_1315 0.36 +0.0 1,498,016 + Pf1N1B4_1315 0.36 -2.8 1,498,016 + Pf1N1B4_1315 0.36 +0.9 1,498,017 - Pf1N1B4_1315 0.36 +0.5 1,498,017 - Pf1N1B4_1315 0.36 -3.4 1,498,017 - Pf1N1B4_1315 0.36 -0.7 1,498,017 - Pf1N1B4_1315 0.36 -1.7
Or see this region's nucleotide sequence