Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03290 and MMP_RS03295 are separated by 9 nucleotides MMP_RS03295 and MMP_RS03300 are separated by 323 nucleotides MMP_RS03300 and MMP_RS03305 are separated by 168 nucleotides MMP_RS03305 and MMP_RS03310 are separated by 177 nucleotides
MMP_RS03290: MMP_RS03290 - AAA family ATPase, at 619,232 to 619,768
_RS03290
MMP_RS03295: MMP_RS03295 - 50S ribosomal protein L34e, at 619,778 to 620,047
_RS03295
MMP_RS03300: MMP_RS03300 - TOBE domain-containing protein, at 620,371 to 620,577
_RS03300
MMP_RS03305: MMP_RS03305 - winged helix-turn-helix domain-containing protein, at 620,746 to 621,144
_RS03305
MMP_RS03310: MMP_RS03310 - ferrous iron transport protein B, at 621,322 to 623,265
_RS03310
Position (kb) 620
621
622 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 620.050 kb on - strand at 620.120 kb on - strand at 620.200 kb on - strand at 620.256 kb on - strand at 620.256 kb on - strand at 620.297 kb on - strand at 620.298 kb on + strand at 620.314 kb on - strand at 620.315 kb on + strand at 620.336 kb on - strand at 620.368 kb on - strand at 620.369 kb on + strand at 620.369 kb on + strand at 620.370 kb on - strand at 620.382 kb on - strand at 620.546 kb on - strand, within MMP_RS03300 at 620.642 kb on - strand at 620.643 kb on + strand at 620.645 kb on + strand at 620.662 kb on + strand at 620.664 kb on + strand at 620.679 kb on + strand at 620.682 kb on - strand at 620.682 kb on - strand at 620.683 kb on + strand at 620.685 kb on + strand at 620.685 kb on + strand at 620.686 kb on - strand at 620.692 kb on - strand at 620.716 kb on + strand at 620.793 kb on + strand, within MMP_RS03305 at 620.793 kb on + strand, within MMP_RS03305 at 620.793 kb on + strand, within MMP_RS03305 at 620.793 kb on + strand, within MMP_RS03305 at 620.835 kb on + strand, within MMP_RS03305 at 620.859 kb on - strand, within MMP_RS03305 at 620.859 kb on - strand, within MMP_RS03305 at 620.859 kb on - strand, within MMP_RS03305 at 620.891 kb on - strand, within MMP_RS03305 at 620.930 kb on + strand, within MMP_RS03305 at 621.127 kb on + strand at 621.142 kb on - strand at 621.167 kb on + strand at 621.196 kb on - strand at 621.239 kb on - strand at 621.239 kb on - strand at 621.267 kb on - strand at 621.268 kb on + strand at 621.268 kb on + strand at 621.271 kb on - strand at 621.273 kb on - strand at 621.323 kb on - strand at 621.334 kb on - strand at 621.334 kb on - strand at 621.350 kb on - strand at 621.350 kb on - strand at 621.355 kb on - strand at 621.390 kb on + strand at 621.855 kb on - strand at 621.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 620,050 - -1.3 620,120 - -0.9 620,200 - -0.5 620,256 - +0.5 620,256 - -0.3 620,297 - +0.3 620,298 + +0.2 620,314 - -0.2 620,315 + +2.2 620,336 - +0.2 620,368 - -0.7 620,369 + +2.6 620,369 + +0.9 620,370 - -0.6 620,382 - +3.6 620,546 - MMP_RS03300 0.85 +0.1 620,642 - +0.5 620,643 + +0.5 620,645 + +0.2 620,662 + +0.8 620,664 + +0.4 620,679 + +0.1 620,682 - +0.4 620,682 - +0.6 620,683 + -1.2 620,685 + +0.9 620,685 + +1.3 620,686 - +1.4 620,692 - +0.8 620,716 + +0.7 620,793 + MMP_RS03305 0.12 +0.6 620,793 + MMP_RS03305 0.12 +0.7 620,793 + MMP_RS03305 0.12 +0.2 620,793 + MMP_RS03305 0.12 +0.5 620,835 + MMP_RS03305 0.22 +0.4 620,859 - MMP_RS03305 0.28 +0.6 620,859 - MMP_RS03305 0.28 +1.9 620,859 - MMP_RS03305 0.28 +0.2 620,891 - MMP_RS03305 0.36 +0.3 620,930 + MMP_RS03305 0.46 +0.9 621,127 + +0.3 621,142 - +0.5 621,167 + -0.1 621,196 - -1.5 621,239 - +0.3 621,239 - -0.1 621,267 - +0.1 621,268 + -0.3 621,268 + -0.1 621,271 - -0.2 621,273 - -0.4 621,323 - -0.3 621,334 - -4.3 621,334 - -4.4 621,350 - -2.9 621,350 - -2.5 621,355 - +0.1 621,390 + -2.2 621,855 - -3.9 621,894 - -3.5
Or see this region's nucleotide sequence