Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02310 and MMP_RS02315 are separated by 0 nucleotides MMP_RS02315 and MMP_RS02320 overlap by 14 nucleotides MMP_RS02320 and MMP_RS02325 overlap by 4 nucleotides MMP_RS02325 and MMP_RS02330 are separated by 93 nucleotides
MMP_RS02310: MMP_RS02310 - RimK-like ATPgrasp N-terminal domain-containing protein, at 435,030 to 435,635
_RS02310
MMP_RS02315: MMP_RS02315 - RimK family alpha-L-glutamate ligase, at 435,636 to 436,586
_RS02315
MMP_RS02320: MMP_RS02320 - glutamate-cysteine ligase family protein, at 436,573 to 437,676
_RS02320
MMP_RS02325: MMP_RS02325 - epoxyqueuosine reductase, at 437,673 to 438,470
_RS02325
MMP_RS02330: MMP_RS02330 - dihydroorotate dehydrogenase, at 438,564 to 439,478
_RS02330
Position (kb) 436
437
438 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 435.922 kb on + strand, within MMP_RS02315 at 436.008 kb on + strand, within MMP_RS02315 at 436.109 kb on + strand, within MMP_RS02315 at 436.278 kb on - strand, within MMP_RS02315 at 436.326 kb on + strand, within MMP_RS02315 at 436.408 kb on + strand, within MMP_RS02315 at 436.473 kb on - strand, within MMP_RS02315 at 436.491 kb on - strand, within MMP_RS02315 at 436.492 kb on + strand at 436.494 kb on + strand at 436.518 kb on - strand at 436.518 kb on - strand at 436.594 kb on - strand at 436.595 kb on + strand at 436.641 kb on + strand at 436.818 kb on - strand, within MMP_RS02320 at 436.818 kb on - strand, within MMP_RS02320 at 436.822 kb on + strand, within MMP_RS02320 at 436.822 kb on + strand, within MMP_RS02320 at 436.832 kb on - strand, within MMP_RS02320 at 436.925 kb on - strand, within MMP_RS02320 at 436.985 kb on + strand, within MMP_RS02320 at 437.007 kb on - strand, within MMP_RS02320 at 437.042 kb on + strand, within MMP_RS02320 at 437.097 kb on - strand, within MMP_RS02320 at 437.098 kb on + strand, within MMP_RS02320 at 437.483 kb on - strand, within MMP_RS02320 at 437.541 kb on + strand, within MMP_RS02320 at 437.586 kb on - strand at 437.587 kb on + strand at 437.627 kb on - strand at 437.667 kb on - strand at 437.674 kb on - strand at 437.726 kb on - strand at 437.914 kb on - strand, within MMP_RS02325 at 437.958 kb on + strand, within MMP_RS02325 at 438.000 kb on - strand, within MMP_RS02325 at 438.001 kb on + strand, within MMP_RS02325 at 438.001 kb on + strand, within MMP_RS02325 at 438.005 kb on - strand, within MMP_RS02325 at 438.005 kb on - strand, within MMP_RS02325 at 438.013 kb on + strand, within MMP_RS02325 at 438.069 kb on - strand, within MMP_RS02325 at 438.277 kb on + strand, within MMP_RS02325 at 438.330 kb on - strand, within MMP_RS02325 at 438.330 kb on - strand, within MMP_RS02325 at 438.330 kb on - strand, within MMP_RS02325 at 438.331 kb on + strand, within MMP_RS02325 at 438.399 kb on + strand at 438.443 kb on - strand at 438.447 kb on + strand at 438.488 kb on + strand at 438.493 kb on - strand at 438.493 kb on - strand at 438.493 kb on - strand at 438.493 kb on - strand at 438.532 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 435,922 + MMP_RS02315 0.30 -0.0 436,008 + MMP_RS02315 0.39 +0.6 436,109 + MMP_RS02315 0.50 +1.0 436,278 - MMP_RS02315 0.68 +0.0 436,326 + MMP_RS02315 0.73 -0.1 436,408 + MMP_RS02315 0.81 +0.5 436,473 - MMP_RS02315 0.88 -0.0 436,491 - MMP_RS02315 0.90 +0.2 436,492 + +0.7 436,494 + +0.1 436,518 - +0.4 436,518 - +0.0 436,594 - -0.2 436,595 + +0.4 436,641 + +0.0 436,818 - MMP_RS02320 0.22 +2.8 436,818 - MMP_RS02320 0.22 +0.2 436,822 + MMP_RS02320 0.23 +0.4 436,822 + MMP_RS02320 0.23 +0.1 436,832 - MMP_RS02320 0.23 +0.3 436,925 - MMP_RS02320 0.32 +0.4 436,985 + MMP_RS02320 0.37 +0.3 437,007 - MMP_RS02320 0.39 -0.2 437,042 + MMP_RS02320 0.42 +0.1 437,097 - MMP_RS02320 0.47 +0.2 437,098 + MMP_RS02320 0.48 +1.2 437,483 - MMP_RS02320 0.82 +0.8 437,541 + MMP_RS02320 0.88 +0.4 437,586 - -0.2 437,587 + +0.1 437,627 - -0.3 437,667 - -0.6 437,674 - +2.5 437,726 - -0.0 437,914 - MMP_RS02325 0.30 +0.8 437,958 + MMP_RS02325 0.36 +0.6 438,000 - MMP_RS02325 0.41 -0.1 438,001 + MMP_RS02325 0.41 -0.2 438,001 + MMP_RS02325 0.41 -0.4 438,005 - MMP_RS02325 0.42 -0.6 438,005 - MMP_RS02325 0.42 +0.3 438,013 + MMP_RS02325 0.43 +0.3 438,069 - MMP_RS02325 0.50 -0.3 438,277 + MMP_RS02325 0.76 -0.3 438,330 - MMP_RS02325 0.82 +0.1 438,330 - MMP_RS02325 0.82 +0.6 438,330 - MMP_RS02325 0.82 +0.2 438,331 + MMP_RS02325 0.82 +0.2 438,399 + +0.5 438,443 - +0.6 438,447 + -0.3 438,488 + -0.1 438,493 - +0.4 438,493 - -1.3 438,493 - +0.3 438,493 - +0.3 438,532 + -4.0
Or see this region's nucleotide sequence