Strain Fitness in Methanococcus maripaludis S2 around MMP_RS02205

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS02200 and MMP_RS02205 are separated by 62 nucleotidesMMP_RS02205 and MMP_RS02210 are separated by 171 nucleotides MMP_RS02200: MMP_RS02200 - methyl-accepting chemotaxis protein, at 411,833 to 414,028 _RS02200 MMP_RS02205: MMP_RS02205 - threonine--tRNA ligase, at 414,091 to 415,959 _RS02205 MMP_RS02210: MMP_RS02210 - bifunctional N(6)-L-threonylcarbamoyladenine synthase/serine/threonine protein kinase, at 416,131 to 417,777 _RS02210 Position (kb) 414 415 416Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 413.407 kb on - strand, within MMP_RS02200at 413.416 kb on + strand, within MMP_RS02200at 413.492 kb on + strand, within MMP_RS02200at 413.688 kb on - strand, within MMP_RS02200at 413.689 kb on + strand, within MMP_RS02200at 414.023 kb on + strandat 414.043 kb on + strandat 414.976 kb on - strandat 415.055 kb on - strandat 415.056 kb on + strandat 415.261 kb on - strandat 415.974 kb on - strandat 415.975 kb on + strandat 416.040 kb on + strandat 416.051 kb on - strandat 416.157 kb on + strandat 416.445 kb on - strandat 416.719 kb on - strandat 416.807 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
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413,407 - MMP_RS02200 0.72 +0.7
413,416 + MMP_RS02200 0.72 -0.2
413,492 + MMP_RS02200 0.76 +0.2
413,688 - MMP_RS02200 0.84 -0.3
413,689 + MMP_RS02200 0.85 +0.5
414,023 + +0.2
414,043 + +3.1
414,976 - -3.6
415,055 - -1.9
415,056 + -2.8
415,261 - -2.8
415,974 - -2.5
415,975 + -3.1
416,040 + -2.9
416,051 - -2.8
416,157 + -3.2
416,445 - -2.4
416,719 - -3.5
416,807 - -2.8

Or see this region's nucleotide sequence