Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01680 and MMP_RS01685 overlap by 8 nucleotides MMP_RS01685 and MMP_RS01690 overlap by 4 nucleotides MMP_RS01690 and MMP_RS01695 are separated by 367 nucleotides
MMP_RS01680: MMP_RS01680 - phenylacetate--CoA ligase, at 308,925 to 310,169
_RS01680
MMP_RS01685: MMP_RS01685 - indolepyruvate oxidoreductase subunit beta, at 310,162 to 310,761
_RS01685
MMP_RS01690: MMP_RS01690 - indolepyruvate ferredoxin oxidoreductase subunit alpha, at 310,758 to 312,596
_RS01690
MMP_RS01695: MMP_RS01695 - fructose-1 6-bisphosphatase, at 312,964 to 314,115
_RS01695
Position (kb) 310
311
312
313 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 309.821 kb on - strand, within MMP_RS01680 at 309.828 kb on + strand, within MMP_RS01680 at 309.879 kb on + strand, within MMP_RS01680 at 309.882 kb on - strand, within MMP_RS01680 at 309.883 kb on + strand, within MMP_RS01680 at 309.898 kb on - strand, within MMP_RS01680 at 309.903 kb on - strand, within MMP_RS01680 at 309.943 kb on + strand, within MMP_RS01680 at 310.049 kb on - strand at 310.050 kb on + strand at 310.065 kb on - strand at 310.065 kb on - strand at 310.065 kb on - strand at 310.397 kb on - strand, within MMP_RS01685 at 310.486 kb on - strand, within MMP_RS01685 at 310.745 kb on + strand at 310.997 kb on + strand, within MMP_RS01690 at 311.111 kb on - strand, within MMP_RS01690 at 311.229 kb on - strand, within MMP_RS01690 at 311.427 kb on + strand, within MMP_RS01690 at 311.619 kb on - strand, within MMP_RS01690 at 311.620 kb on + strand, within MMP_RS01690 at 311.860 kb on + strand, within MMP_RS01690 at 312.075 kb on - strand, within MMP_RS01690 at 312.107 kb on - strand, within MMP_RS01690 at 312.313 kb on - strand, within MMP_RS01690 at 312.313 kb on - strand, within MMP_RS01690 at 312.313 kb on - strand, within MMP_RS01690 at 312.314 kb on + strand, within MMP_RS01690 at 312.370 kb on + strand, within MMP_RS01690 at 312.455 kb on - strand at 312.609 kb on - strand at 312.613 kb on + strand at 312.671 kb on + strand at 312.671 kb on + strand at 312.672 kb on - strand at 312.673 kb on + strand at 312.673 kb on + strand at 312.674 kb on - strand at 312.674 kb on - strand at 312.680 kb on + strand at 312.711 kb on + strand at 312.776 kb on + strand at 312.786 kb on + strand at 312.790 kb on - strand at 312.819 kb on - strand at 312.823 kb on - strand at 312.842 kb on - strand at 312.843 kb on + strand at 312.843 kb on + strand at 312.843 kb on + strand at 312.895 kb on + strand at 312.912 kb on - strand at 312.912 kb on - strand at 312.918 kb on - strand at 312.984 kb on + strand at 313.029 kb on + strand at 313.435 kb on - strand at 313.525 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 309,821 - MMP_RS01680 0.72 +0.4 309,828 + MMP_RS01680 0.73 +0.1 309,879 + MMP_RS01680 0.77 +0.1 309,882 - MMP_RS01680 0.77 +0.2 309,883 + MMP_RS01680 0.77 +0.0 309,898 - MMP_RS01680 0.78 +0.0 309,903 - MMP_RS01680 0.79 -0.1 309,943 + MMP_RS01680 0.82 +0.2 310,049 - +0.1 310,050 + -0.1 310,065 - +0.6 310,065 - +0.3 310,065 - -0.6 310,397 - MMP_RS01685 0.39 +0.3 310,486 - MMP_RS01685 0.54 -0.0 310,745 + +0.1 310,997 + MMP_RS01690 0.13 +0.1 311,111 - MMP_RS01690 0.19 +0.4 311,229 - MMP_RS01690 0.26 +0.4 311,427 + MMP_RS01690 0.36 +0.8 311,619 - MMP_RS01690 0.47 +2.4 311,620 + MMP_RS01690 0.47 +0.1 311,860 + MMP_RS01690 0.60 -0.2 312,075 - MMP_RS01690 0.72 +0.4 312,107 - MMP_RS01690 0.73 -0.3 312,313 - MMP_RS01690 0.85 +0.6 312,313 - MMP_RS01690 0.85 +2.6 312,313 - MMP_RS01690 0.85 -0.1 312,314 + MMP_RS01690 0.85 -0.1 312,370 + MMP_RS01690 0.88 +0.0 312,455 - -0.1 312,609 - -0.3 312,613 + +0.3 312,671 + +0.6 312,671 + +0.1 312,672 - +2.9 312,673 + +0.0 312,673 + +0.3 312,674 - +0.3 312,674 - +3.0 312,680 + +0.3 312,711 + +3.0 312,776 + -0.4 312,786 + +0.0 312,790 - -0.5 312,819 - -0.3 312,823 - -5.2 312,842 - -3.3 312,843 + -1.5 312,843 + -3.3 312,843 + -0.6 312,895 + -2.9 312,912 - +0.0 312,912 - -1.8 312,918 - -2.6 312,984 + -2.7 313,029 + -2.7 313,435 - -1.0 313,525 - -3.2
Or see this region's nucleotide sequence