Experiment: formate McAA biotin dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02270 and MMJJ_RS02275 overlap by 14 nucleotides MMJJ_RS02275 and MMJJ_RS02280 are separated by 231 nucleotides MMJJ_RS02280 and MMJJ_RS02285 are separated by 423 nucleotides MMJJ_RS02285 and MMJJ_RS02290 are separated by 1 nucleotides
MMJJ_RS02270: MMJJ_RS02270 - HEPN domain-containing protein, at 429,486 to 429,995
_RS02270
MMJJ_RS02275: MMJJ_RS02275 - nucleotidyltransferase domain-containing protein, at 429,982 to 430,599
_RS02275
MMJJ_RS02280: MMJJ_RS02280 - sulfate permease, at 430,831 to 432,489
_RS02280
MMJJ_RS02285: MMJJ_RS02285 - class III signal peptide-containing protein, at 432,913 to 433,134
_RS02285
MMJJ_RS02290: MMJJ_RS02290 - MarR family transcriptional regulator, at 433,136 to 433,762
_RS02290
Position (kb) 430
431
432
433 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 429.843 kb on - strand, within MMJJ_RS02270 at 429.858 kb on + strand, within MMJJ_RS02270 at 429.943 kb on - strand, within MMJJ_RS02270 at 429.976 kb on - strand at 430.109 kb on + strand, within MMJJ_RS02275 at 430.111 kb on + strand, within MMJJ_RS02275 at 430.111 kb on + strand, within MMJJ_RS02275 at 430.111 kb on + strand, within MMJJ_RS02275 at 430.114 kb on - strand, within MMJJ_RS02275 at 430.350 kb on - strand, within MMJJ_RS02275 at 430.350 kb on - strand, within MMJJ_RS02275 at 430.424 kb on - strand, within MMJJ_RS02275 at 430.447 kb on - strand, within MMJJ_RS02275 at 430.536 kb on - strand, within MMJJ_RS02275 at 430.566 kb on + strand at 430.568 kb on - strand at 430.569 kb on + strand at 430.579 kb on - strand at 430.613 kb on + strand at 430.619 kb on + strand at 430.622 kb on - strand at 430.622 kb on - strand at 430.622 kb on - strand at 430.623 kb on + strand at 430.623 kb on + strand at 430.624 kb on - strand at 430.680 kb on - strand at 430.680 kb on - strand at 430.693 kb on - strand at 430.693 kb on - strand at 430.693 kb on - strand at 430.694 kb on + strand at 430.698 kb on + strand at 430.699 kb on - strand at 430.700 kb on + strand at 430.700 kb on + strand at 430.700 kb on + strand at 430.700 kb on + strand at 430.705 kb on + strand at 430.710 kb on + strand at 430.722 kb on + strand at 430.736 kb on + strand at 430.739 kb on - strand at 430.740 kb on + strand at 430.740 kb on + strand at 430.740 kb on + strand at 430.741 kb on - strand at 430.741 kb on - strand at 430.742 kb on + strand at 430.743 kb on - strand at 430.744 kb on + strand at 430.762 kb on - strand at 430.764 kb on - strand at 430.765 kb on + strand at 430.765 kb on + strand at 430.787 kb on + strand at 430.790 kb on - strand at 430.796 kb on + strand at 430.796 kb on + strand at 430.808 kb on + strand at 430.808 kb on + strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.828 kb on - strand at 430.829 kb on + strand at 430.829 kb on + strand at 430.846 kb on + strand at 430.878 kb on + strand at 430.901 kb on - strand at 430.902 kb on + strand at 430.964 kb on - strand at 430.992 kb on + strand at 430.992 kb on + strand at 431.073 kb on - strand, within MMJJ_RS02280 at 431.074 kb on + strand, within MMJJ_RS02280 at 431.126 kb on - strand, within MMJJ_RS02280 at 431.160 kb on - strand, within MMJJ_RS02280 at 431.196 kb on - strand, within MMJJ_RS02280 at 431.197 kb on + strand, within MMJJ_RS02280 at 431.217 kb on + strand, within MMJJ_RS02280 at 431.295 kb on - strand, within MMJJ_RS02280 at 431.295 kb on - strand, within MMJJ_RS02280 at 431.299 kb on - strand, within MMJJ_RS02280 at 431.300 kb on + strand, within MMJJ_RS02280 at 431.300 kb on + strand, within MMJJ_RS02280 at 431.300 kb on + strand, within MMJJ_RS02280 at 431.300 kb on + strand, within MMJJ_RS02280 at 431.310 kb on - strand, within MMJJ_RS02280 at 431.321 kb on - strand, within MMJJ_RS02280 at 431.321 kb on - strand, within MMJJ_RS02280 at 431.321 kb on - strand, within MMJJ_RS02280 at 431.322 kb on + strand, within MMJJ_RS02280 at 431.358 kb on + strand, within MMJJ_RS02280 at 431.362 kb on + strand, within MMJJ_RS02280 at 431.408 kb on - strand, within MMJJ_RS02280 at 431.417 kb on - strand, within MMJJ_RS02280 at 431.442 kb on - strand, within MMJJ_RS02280 at 431.443 kb on + strand, within MMJJ_RS02280 at 431.443 kb on + strand at 431.443 kb on + strand, within MMJJ_RS02280 at 431.454 kb on + strand, within MMJJ_RS02280 at 431.476 kb on + strand, within MMJJ_RS02280 at 431.568 kb on - strand, within MMJJ_RS02280 at 431.588 kb on - strand, within MMJJ_RS02280 at 431.634 kb on - strand, within MMJJ_RS02280 at 431.635 kb on + strand, within MMJJ_RS02280 at 431.679 kb on - strand, within MMJJ_RS02280 at 431.679 kb on - strand, within MMJJ_RS02280 at 431.680 kb on + strand, within MMJJ_RS02280 at 431.862 kb on + strand, within MMJJ_RS02280 at 431.868 kb on + strand, within MMJJ_RS02280 at 431.905 kb on + strand, within MMJJ_RS02280 at 431.919 kb on - strand, within MMJJ_RS02280 at 431.919 kb on - strand, within MMJJ_RS02280 at 431.920 kb on + strand, within MMJJ_RS02280 at 431.996 kb on - strand, within MMJJ_RS02280 at 432.026 kb on - strand, within MMJJ_RS02280 at 432.105 kb on + strand, within MMJJ_RS02280 at 432.190 kb on + strand, within MMJJ_RS02280 at 432.254 kb on - strand, within MMJJ_RS02280 at 432.254 kb on - strand, within MMJJ_RS02280 at 432.315 kb on - strand, within MMJJ_RS02280 at 432.315 kb on - strand, within MMJJ_RS02280 at 432.316 kb on + strand, within MMJJ_RS02280 at 432.328 kb on + strand at 432.350 kb on - strand at 432.378 kb on - strand at 432.378 kb on - strand at 432.402 kb on - strand at 432.423 kb on + strand at 432.446 kb on - strand at 432.447 kb on + strand at 432.450 kb on + strand at 432.455 kb on - strand at 432.456 kb on + strand at 432.477 kb on + strand at 432.562 kb on - strand at 432.617 kb on + strand at 432.621 kb on + strand at 432.657 kb on - strand at 432.706 kb on - strand at 432.706 kb on - strand at 432.707 kb on + strand at 432.710 kb on - strand at 432.756 kb on - strand at 432.757 kb on + strand at 432.811 kb on - strand at 432.812 kb on + strand at 432.817 kb on - strand at 432.817 kb on - strand at 432.818 kb on + strand at 432.818 kb on + strand at 432.818 kb on + strand at 432.838 kb on - strand at 432.839 kb on + strand at 432.839 kb on + strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.844 kb on - strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.845 kb on + strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.846 kb on - strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.847 kb on + strand at 432.848 kb on - strand at 432.848 kb on - strand at 432.848 kb on - strand at 432.848 kb on - strand at 432.848 kb on - strand at 432.849 kb on + strand at 432.849 kb on + strand at 432.849 kb on + strand at 432.849 kb on + strand at 432.849 kb on + strand at 432.849 kb on + strand at 432.849 kb on + strand at 432.849 kb on + strand at 432.849 kb on + strand at 432.862 kb on - strand at 432.868 kb on - strand at 432.869 kb on + strand at 432.869 kb on + strand at 432.869 kb on + strand at 432.869 kb on + strand at 432.873 kb on + strand at 432.873 kb on + strand at 432.887 kb on + strand at 432.895 kb on - strand at 432.912 kb on - strand at 432.913 kb on + strand at 433.019 kb on - strand, within MMJJ_RS02285 at 433.019 kb on - strand, within MMJJ_RS02285 at 433.020 kb on + strand, within MMJJ_RS02285 at 433.027 kb on + strand, within MMJJ_RS02285 at 433.082 kb on - strand, within MMJJ_RS02285 at 433.083 kb on + strand, within MMJJ_RS02285 at 433.121 kb on - strand at 433.121 kb on - strand at 433.125 kb on - strand at 433.126 kb on + strand at 433.126 kb on + strand at 433.133 kb on + strand at 433.138 kb on + strand at 433.139 kb on - strand at 433.162 kb on - strand at 433.163 kb on + strand at 433.166 kb on - strand at 433.180 kb on + strand at 433.185 kb on + strand at 433.199 kb on + strand, within MMJJ_RS02290 at 433.206 kb on + strand, within MMJJ_RS02290 at 433.215 kb on + strand, within MMJJ_RS02290 at 433.295 kb on - strand, within MMJJ_RS02290 at 433.296 kb on + strand, within MMJJ_RS02290 at 433.302 kb on + strand, within MMJJ_RS02290 at 433.352 kb on - strand, within MMJJ_RS02290 at 433.472 kb on + strand, within MMJJ_RS02290 at 433.472 kb on + strand, within MMJJ_RS02290
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA biotin dropout remove 429,843 - MMJJ_RS02270 0.70 -0.0 429,858 + MMJJ_RS02270 0.73 -0.4 429,943 - MMJJ_RS02270 0.90 -0.0 429,976 - -0.5 430,109 + MMJJ_RS02275 0.21 -3.1 430,111 + MMJJ_RS02275 0.21 -2.9 430,111 + MMJJ_RS02275 0.21 -2.7 430,111 + MMJJ_RS02275 0.21 -1.7 430,114 - MMJJ_RS02275 0.21 -1.4 430,350 - MMJJ_RS02275 0.60 -2.8 430,350 - MMJJ_RS02275 0.60 -3.4 430,424 - MMJJ_RS02275 0.72 -2.1 430,447 - MMJJ_RS02275 0.75 -4.2 430,536 - MMJJ_RS02275 0.90 -1.1 430,566 + -2.4 430,568 - -3.1 430,569 + -2.0 430,579 - -1.4 430,613 + -3.4 430,619 + -3.3 430,622 - -2.7 430,622 - -3.4 430,622 - -3.4 430,623 + -2.7 430,623 + -2.4 430,624 - -3.6 430,680 - -0.1 430,680 - +0.5 430,693 - +0.7 430,693 - -0.9 430,693 - +0.1 430,694 + +0.3 430,698 + -0.4 430,699 - +0.0 430,700 + +0.9 430,700 + +0.0 430,700 + +2.0 430,700 + -0.8 430,705 + -1.2 430,710 + -1.2 430,722 + +0.5 430,736 + -0.7 430,739 - +0.0 430,740 + -1.0 430,740 + -1.3 430,740 + +0.5 430,741 - +0.1 430,741 - -0.1 430,742 + -0.4 430,743 - +0.4 430,744 + -0.4 430,762 - +0.6 430,764 - +0.2 430,765 + +0.2 430,765 + -2.3 430,787 + -0.2 430,790 - -0.6 430,796 + -0.3 430,796 + +1.3 430,808 + -0.6 430,808 + +0.6 430,828 - +0.5 430,828 - -0.3 430,828 - +0.8 430,828 - +0.4 430,828 - +0.1 430,828 - -0.4 430,828 - -0.2 430,828 - +0.9 430,829 + -0.4 430,829 + -2.0 430,846 + +0.9 430,878 + +0.2 430,901 - +0.0 430,902 + -0.2 430,964 - -0.3 430,992 + -0.4 430,992 + +0.2 431,073 - MMJJ_RS02280 0.15 +0.5 431,074 + MMJJ_RS02280 0.15 +0.5 431,126 - MMJJ_RS02280 0.18 -0.5 431,160 - MMJJ_RS02280 0.20 +0.3 431,196 - MMJJ_RS02280 0.22 -0.1 431,197 + MMJJ_RS02280 0.22 -0.2 431,217 + MMJJ_RS02280 0.23 -0.3 431,295 - MMJJ_RS02280 0.28 -0.2 431,295 - MMJJ_RS02280 0.28 +0.1 431,299 - MMJJ_RS02280 0.28 +1.0 431,300 + MMJJ_RS02280 0.28 -0.4 431,300 + MMJJ_RS02280 0.28 -0.1 431,300 + MMJJ_RS02280 0.28 +0.3 431,300 + MMJJ_RS02280 0.28 +0.6 431,310 - MMJJ_RS02280 0.29 +0.2 431,321 - MMJJ_RS02280 0.30 +0.9 431,321 - MMJJ_RS02280 0.30 +0.1 431,321 - MMJJ_RS02280 0.30 +2.1 431,322 + MMJJ_RS02280 0.30 +0.3 431,358 + MMJJ_RS02280 0.32 -1.3 431,362 + MMJJ_RS02280 0.32 -0.3 431,408 - MMJJ_RS02280 0.35 +1.3 431,417 - MMJJ_RS02280 0.35 +0.5 431,442 - MMJJ_RS02280 0.37 +0.5 431,443 + MMJJ_RS02280 0.37 +0.6 431,443 + -1.6 431,443 + MMJJ_RS02280 0.37 +0.5 431,454 + MMJJ_RS02280 0.38 +1.0 431,476 + MMJJ_RS02280 0.39 -0.0 431,568 - MMJJ_RS02280 0.44 -1.0 431,588 - MMJJ_RS02280 0.46 -1.0 431,634 - MMJJ_RS02280 0.48 +0.2 431,635 + MMJJ_RS02280 0.48 -1.2 431,679 - MMJJ_RS02280 0.51 +0.3 431,679 - MMJJ_RS02280 0.51 +0.7 431,680 + MMJJ_RS02280 0.51 +0.1 431,862 + MMJJ_RS02280 0.62 +0.4 431,868 + MMJJ_RS02280 0.63 +0.2 431,905 + MMJJ_RS02280 0.65 +1.5 431,919 - MMJJ_RS02280 0.66 -1.8 431,919 - MMJJ_RS02280 0.66 +0.9 431,920 + MMJJ_RS02280 0.66 +0.5 431,996 - MMJJ_RS02280 0.70 -0.1 432,026 - MMJJ_RS02280 0.72 -0.8 432,105 + MMJJ_RS02280 0.77 +0.9 432,190 + MMJJ_RS02280 0.82 -1.3 432,254 - MMJJ_RS02280 0.86 -0.0 432,254 - MMJJ_RS02280 0.86 +0.7 432,315 - MMJJ_RS02280 0.89 -0.1 432,315 - MMJJ_RS02280 0.89 +0.5 432,316 + MMJJ_RS02280 0.90 +0.7 432,328 + +0.3 432,350 - -0.0 432,378 - +1.2 432,378 - +0.4 432,402 - +1.0 432,423 + +0.6 432,446 - +0.1 432,447 + +0.5 432,450 + +0.3 432,455 - +0.6 432,456 + +0.3 432,477 + +0.4 432,562 - -0.5 432,617 + +0.2 432,621 + -0.4 432,657 - +0.5 432,706 - +0.4 432,706 - -0.2 432,707 + -0.8 432,710 - -0.3 432,756 - +0.3 432,757 + +0.1 432,811 - +0.2 432,812 + -0.9 432,817 - -0.3 432,817 - +0.1 432,818 + +0.2 432,818 + -0.7 432,818 + -0.1 432,838 - +0.0 432,839 + +0.2 432,839 + -0.0 432,844 - -0.1 432,844 - +0.5 432,844 - +0.1 432,844 - -0.3 432,844 - -1.6 432,844 - -0.8 432,844 - -0.6 432,844 - +1.3 432,844 - +0.3 432,844 - +0.8 432,844 - +0.1 432,844 - +0.6 432,844 - -2.0 432,844 - +0.0 432,844 - +0.4 432,844 - +0.5 432,845 + +0.0 432,845 + +0.3 432,845 + +1.8 432,845 + +0.1 432,845 + +0.1 432,845 + -0.5 432,845 + +0.1 432,845 + +0.5 432,845 + -1.0 432,845 + -0.6 432,845 + -0.3 432,845 + +0.9 432,845 + +0.7 432,845 + -0.6 432,845 + -1.0 432,845 + +0.6 432,845 + +0.3 432,845 + -0.0 432,846 - +0.3 432,846 - -0.1 432,846 - +0.6 432,846 - +1.1 432,846 - +0.2 432,846 - -0.5 432,846 - -0.2 432,846 - -0.6 432,846 - +0.2 432,846 - +0.1 432,846 - +0.4 432,846 - -0.7 432,847 + -0.5 432,847 + +0.3 432,847 + +0.2 432,847 + +1.0 432,847 + +0.9 432,847 + -0.0 432,847 + +0.1 432,847 + -0.7 432,847 + +0.1 432,847 + -0.6 432,847 + -0.3 432,848 - -1.0 432,848 - +0.6 432,848 - -0.1 432,848 - +0.4 432,848 - +0.8 432,849 + +1.0 432,849 + +0.6 432,849 + +1.6 432,849 + +0.3 432,849 + -0.1 432,849 + -0.1 432,849 + -0.2 432,849 + -0.0 432,849 + +1.0 432,862 - +1.0 432,868 - -0.2 432,869 + -0.2 432,869 + +1.2 432,869 + -0.5 432,869 + -0.3 432,873 + -0.1 432,873 + -1.0 432,887 + -0.5 432,895 - -0.4 432,912 - +0.7 432,913 + -0.1 433,019 - MMJJ_RS02285 0.48 +1.3 433,019 - MMJJ_RS02285 0.48 -0.5 433,020 + MMJJ_RS02285 0.48 -0.5 433,027 + MMJJ_RS02285 0.51 -0.3 433,082 - MMJJ_RS02285 0.76 +1.3 433,083 + MMJJ_RS02285 0.77 -0.6 433,121 - -0.3 433,121 - +0.3 433,125 - +0.5 433,126 + +0.1 433,126 + +0.7 433,133 + +0.0 433,138 + +0.6 433,139 - -0.8 433,162 - -0.2 433,163 + -0.1 433,166 - +0.3 433,180 + +0.9 433,185 + +1.5 433,199 + MMJJ_RS02290 0.10 +1.0 433,206 + MMJJ_RS02290 0.11 +0.3 433,215 + MMJJ_RS02290 0.13 -0.3 433,295 - MMJJ_RS02290 0.25 +0.5 433,296 + MMJJ_RS02290 0.26 -2.4 433,302 + MMJJ_RS02290 0.26 +0.2 433,352 - MMJJ_RS02290 0.34 -0.2 433,472 + MMJJ_RS02290 0.54 -0.1 433,472 + MMJJ_RS02290 0.54 -0.6
Or see this region's nucleotide sequence