Experiment: PYE with nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00497 and CCNA_00498 are separated by 60 nucleotides CCNA_00498 and CCNA_00499 are separated by 75 nucleotides CCNA_00499 and CCNA_00500 overlap by 4 nucleotides CCNA_00500 and CCNA_00501 overlap by 4 nucleotides
CCNA_00497: CCNA_00497 - putative rhamnosyl transferase, at 514,298 to 515,224
_00497
CCNA_00498: CCNA_00498 - hypothetical protein, at 515,285 to 515,404
_00498
CCNA_00499: CCNA_00499 - hypothetical protein, at 515,480 to 515,845
_00499
CCNA_00500: CCNA_00500 - hydroxymethylglutaryl-CoA lyase, at 515,842 to 516,741
_00500
CCNA_00501: CCNA_00501 - glucosyltransferase, at 516,738 to 518,417
_00501
Position (kb)
515
516
517 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 514.852 kb on - strand, within CCNA_00497 at 514.937 kb on - strand, within CCNA_00497 at 514.980 kb on - strand, within CCNA_00497 at 514.998 kb on + strand, within CCNA_00497 at 515.003 kb on + strand, within CCNA_00497 at 515.003 kb on + strand, within CCNA_00497 at 515.008 kb on + strand, within CCNA_00497 at 515.011 kb on - strand, within CCNA_00497 at 515.014 kb on + strand, within CCNA_00497 at 515.028 kb on - strand, within CCNA_00497 at 515.039 kb on + strand, within CCNA_00497 at 515.047 kb on - strand, within CCNA_00497 at 515.049 kb on + strand, within CCNA_00497 at 515.049 kb on + strand, within CCNA_00497 at 515.057 kb on - strand, within CCNA_00497 at 515.077 kb on + strand, within CCNA_00497 at 515.077 kb on - strand, within CCNA_00497 at 515.084 kb on + strand, within CCNA_00497 at 515.085 kb on - strand, within CCNA_00497 at 515.090 kb on + strand, within CCNA_00497 at 515.090 kb on + strand, within CCNA_00497 at 515.090 kb on + strand, within CCNA_00497 at 515.155 kb on - strand at 515.220 kb on + strand at 515.230 kb on + strand at 515.231 kb on + strand at 515.238 kb on - strand at 515.257 kb on - strand at 515.277 kb on + strand at 515.290 kb on - strand at 515.297 kb on + strand, within CCNA_00498 at 515.300 kb on - strand, within CCNA_00498 at 515.349 kb on + strand, within CCNA_00498 at 515.349 kb on + strand, within CCNA_00498 at 515.357 kb on - strand, within CCNA_00498 at 515.368 kb on + strand, within CCNA_00498 at 515.374 kb on + strand, within CCNA_00498 at 515.382 kb on - strand, within CCNA_00498 at 515.443 kb on - strand at 515.465 kb on + strand at 515.538 kb on + strand, within CCNA_00499 at 515.546 kb on - strand, within CCNA_00499 at 515.565 kb on + strand, within CCNA_00499 at 515.565 kb on + strand, within CCNA_00499 at 515.582 kb on - strand, within CCNA_00499 at 515.586 kb on + strand, within CCNA_00499 at 515.604 kb on + strand, within CCNA_00499 at 515.640 kb on - strand, within CCNA_00499 at 515.642 kb on + strand, within CCNA_00499 at 515.642 kb on + strand, within CCNA_00499 at 515.642 kb on + strand, within CCNA_00499 at 515.689 kb on - strand, within CCNA_00499 at 515.713 kb on + strand, within CCNA_00499 at 515.713 kb on + strand, within CCNA_00499 at 515.721 kb on - strand, within CCNA_00499 at 515.767 kb on + strand, within CCNA_00499 at 515.820 kb on - strand at 515.846 kb on - strand at 516.744 kb on - strand at 516.744 kb on - strand at 516.744 kb on - strand at 516.748 kb on + strand at 516.768 kb on - strand at 516.820 kb on - strand at 516.821 kb on - strand at 516.821 kb on - strand at 516.856 kb on + strand at 516.861 kb on + strand at 516.934 kb on - strand, within CCNA_00501 at 517.036 kb on - strand, within CCNA_00501 at 517.068 kb on + strand, within CCNA_00501 at 517.164 kb on - strand, within CCNA_00501 at 517.224 kb on - strand, within CCNA_00501 at 517.229 kb on + strand, within CCNA_00501 at 517.254 kb on - strand, within CCNA_00501 at 517.317 kb on + strand, within CCNA_00501 at 517.317 kb on + strand, within CCNA_00501 at 517.392 kb on - strand, within CCNA_00501 at 517.403 kb on - strand, within CCNA_00501 at 517.473 kb on - strand, within CCNA_00501 at 517.521 kb on + strand, within CCNA_00501 at 517.586 kb on - strand, within CCNA_00501 at 517.586 kb on - strand, within CCNA_00501 at 517.671 kb on - strand, within CCNA_00501 at 517.710 kb on - strand, within CCNA_00501 at 517.710 kb on - strand, within CCNA_00501 at 517.710 kb on - strand, within CCNA_00501 at 517.710 kb on - strand, within CCNA_00501 at 517.710 kb on - strand, within CCNA_00501 at 517.716 kb on - strand, within CCNA_00501
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrate 100 mM remove 514,852 - CCNA_00497 0.60 -2.6 514,937 - CCNA_00497 0.69 +0.6 514,980 - CCNA_00497 0.74 -1.7 514,998 + CCNA_00497 0.76 +1.8 515,003 + CCNA_00497 0.76 +1.8 515,003 + CCNA_00497 0.76 +1.5 515,008 + CCNA_00497 0.77 +0.8 515,011 - CCNA_00497 0.77 +1.5 515,014 + CCNA_00497 0.77 +1.5 515,028 - CCNA_00497 0.79 +0.1 515,039 + CCNA_00497 0.80 +1.8 515,047 - CCNA_00497 0.81 -0.6 515,049 + CCNA_00497 0.81 +1.1 515,049 + CCNA_00497 0.81 +1.3 515,057 - CCNA_00497 0.82 -0.9 515,077 + CCNA_00497 0.84 +1.4 515,077 - CCNA_00497 0.84 +0.6 515,084 + CCNA_00497 0.85 +1.8 515,085 - CCNA_00497 0.85 -0.8 515,090 + CCNA_00497 0.85 +1.7 515,090 + CCNA_00497 0.85 +1.5 515,090 + CCNA_00497 0.85 +1.4 515,155 - -1.6 515,220 + +2.2 515,230 + +0.9 515,231 + +1.6 515,238 - +2.1 515,257 - +0.6 515,277 + +1.7 515,290 - -1.0 515,297 + CCNA_00498 0.10 +2.1 515,300 - CCNA_00498 0.12 -0.2 515,349 + CCNA_00498 0.53 +0.7 515,349 + CCNA_00498 0.53 +0.4 515,357 - CCNA_00498 0.60 -0.0 515,368 + CCNA_00498 0.69 -1.6 515,374 + CCNA_00498 0.74 -0.4 515,382 - CCNA_00498 0.81 +0.4 515,443 - -0.1 515,465 + -0.2 515,538 + CCNA_00499 0.16 +1.8 515,546 - CCNA_00499 0.18 -0.4 515,565 + CCNA_00499 0.23 +1.7 515,565 + CCNA_00499 0.23 +0.6 515,582 - CCNA_00499 0.28 -0.4 515,586 + CCNA_00499 0.29 -0.3 515,604 + CCNA_00499 0.34 +0.8 515,640 - CCNA_00499 0.44 +0.2 515,642 + CCNA_00499 0.44 +0.2 515,642 + CCNA_00499 0.44 +0.4 515,642 + CCNA_00499 0.44 -0.8 515,689 - CCNA_00499 0.57 +1.8 515,713 + CCNA_00499 0.64 +0.6 515,713 + CCNA_00499 0.64 -0.8 515,721 - CCNA_00499 0.66 +0.9 515,767 + CCNA_00499 0.78 -0.3 515,820 - +0.8 515,846 - +0.8 516,744 - +1.1 516,744 - +0.9 516,744 - +1.2 516,748 + +0.1 516,768 - -0.6 516,820 - -0.6 516,821 - -1.0 516,821 - -3.2 516,856 + -1.4 516,861 + -1.7 516,934 - CCNA_00501 0.12 +1.2 517,036 - CCNA_00501 0.18 -0.3 517,068 + CCNA_00501 0.20 -1.7 517,164 - CCNA_00501 0.25 -2.0 517,224 - CCNA_00501 0.29 -1.3 517,229 + CCNA_00501 0.29 -0.0 517,254 - CCNA_00501 0.31 -2.8 517,317 + CCNA_00501 0.34 -0.1 517,317 + CCNA_00501 0.34 -0.4 517,392 - CCNA_00501 0.39 -2.0 517,403 - CCNA_00501 0.40 -1.5 517,473 - CCNA_00501 0.44 -1.4 517,521 + CCNA_00501 0.47 -1.0 517,586 - CCNA_00501 0.50 -1.4 517,586 - CCNA_00501 0.50 -2.1 517,671 - CCNA_00501 0.56 -0.0 517,710 - CCNA_00501 0.58 -0.4 517,710 - CCNA_00501 0.58 -2.1 517,710 - CCNA_00501 0.58 -1.3 517,710 - CCNA_00501 0.58 -0.6 517,710 - CCNA_00501 0.58 -3.1 517,716 - CCNA_00501 0.58 -0.9
Or see this region's nucleotide sequence