Experiment: Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS19210 and ABZR86_RS19215 are separated by 190 nucleotides ABZR86_RS19215 and ABZR86_RS19220 are separated by 60 nucleotides ABZR86_RS19220 and ABZR86_RS19225 are separated by 44 nucleotides
ABZR86_RS19210: ABZR86_RS19210 - polyhydroxyalkanoate depolymerase, at 296,712 to 297,965
_RS19210
ABZR86_RS19215: ABZR86_RS19215 - class I SAM-dependent methyltransferase, at 298,156 to 298,902
_RS19215
ABZR86_RS19220: ABZR86_RS19220 - RDD family protein, at 298,963 to 299,469
_RS19220
ABZR86_RS19225: ABZR86_RS19225 - site-specific tyrosine recombinase XerD, at 299,514 to 300,425
_RS19225
Position (kb) 298
299
300 Strain fitness (log2 ratio) -2
-1
0
1 at 298.001 kb on + strand at 298.001 kb on + strand at 298.053 kb on + strand at 298.054 kb on - strand at 298.070 kb on + strand at 298.116 kb on + strand at 298.116 kb on + strand at 298.157 kb on + strand at 298.158 kb on - strand at 298.158 kb on - strand at 298.271 kb on + strand, within ABZR86_RS19215 at 298.271 kb on + strand, within ABZR86_RS19215 at 298.271 kb on + strand, within ABZR86_RS19215 at 298.272 kb on - strand, within ABZR86_RS19215 at 298.272 kb on - strand, within ABZR86_RS19215 at 298.272 kb on - strand, within ABZR86_RS19215 at 298.307 kb on + strand, within ABZR86_RS19215 at 298.308 kb on - strand, within ABZR86_RS19215 at 298.308 kb on - strand, within ABZR86_RS19215 at 298.308 kb on - strand, within ABZR86_RS19215 at 298.355 kb on + strand, within ABZR86_RS19215 at 298.356 kb on - strand, within ABZR86_RS19215 at 298.356 kb on - strand, within ABZR86_RS19215 at 298.604 kb on + strand, within ABZR86_RS19215 at 298.604 kb on + strand, within ABZR86_RS19215 at 298.605 kb on - strand, within ABZR86_RS19215 at 298.785 kb on + strand, within ABZR86_RS19215 at 298.785 kb on + strand, within ABZR86_RS19215 at 298.836 kb on - strand at 298.836 kb on - strand at 298.836 kb on - strand at 298.908 kb on + strand at 298.909 kb on - strand at 299.255 kb on + strand, within ABZR86_RS19220 at 299.255 kb on + strand, within ABZR86_RS19220 at 299.256 kb on - strand, within ABZR86_RS19220 at 299.289 kb on - strand, within ABZR86_RS19220 at 299.357 kb on + strand, within ABZR86_RS19220 at 299.358 kb on - strand, within ABZR86_RS19220 at 299.358 kb on - strand, within ABZR86_RS19220 at 299.358 kb on - strand, within ABZR86_RS19220 at 299.399 kb on + strand, within ABZR86_RS19220 at 299.400 kb on - strand, within ABZR86_RS19220 at 299.495 kb on + strand at 299.534 kb on + strand at 299.534 kb on + strand at 299.534 kb on + strand at 299.535 kb on - strand at 299.535 kb on - strand at 299.535 kb on - strand at 299.709 kb on + strand, within ABZR86_RS19225 at 299.773 kb on - strand, within ABZR86_RS19225 at 299.773 kb on - strand, within ABZR86_RS19225 at 299.776 kb on - strand, within ABZR86_RS19225 at 299.955 kb on + strand, within ABZR86_RS19225 at 299.956 kb on - strand, within ABZR86_RS19225 at 299.956 kb on - strand, within ABZR86_RS19225 at 299.956 kb on - strand, within ABZR86_RS19225 at 300.103 kb on - strand, within ABZR86_RS19225 at 300.363 kb on + strand at 300.364 kb on - strand at 300.364 kb on - strand at 300.364 kb on - strand at 300.365 kb on + strand at 300.366 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.02 mg/ml remove 298,001 + -0.3 298,001 + -0.7 298,053 + +0.0 298,054 - +0.1 298,070 + -1.3 298,116 + -0.1 298,116 + +0.7 298,157 + +0.2 298,158 - +0.2 298,158 - +1.2 298,271 + ABZR86_RS19215 0.15 -0.7 298,271 + ABZR86_RS19215 0.15 -0.5 298,271 + ABZR86_RS19215 0.15 -0.3 298,272 - ABZR86_RS19215 0.16 -0.2 298,272 - ABZR86_RS19215 0.16 -0.9 298,272 - ABZR86_RS19215 0.16 -0.2 298,307 + ABZR86_RS19215 0.20 +0.9 298,308 - ABZR86_RS19215 0.20 +0.0 298,308 - ABZR86_RS19215 0.20 -0.1 298,308 - ABZR86_RS19215 0.20 -0.1 298,355 + ABZR86_RS19215 0.27 -0.6 298,356 - ABZR86_RS19215 0.27 +0.2 298,356 - ABZR86_RS19215 0.27 -0.2 298,604 + ABZR86_RS19215 0.60 +0.3 298,604 + ABZR86_RS19215 0.60 -0.1 298,605 - ABZR86_RS19215 0.60 +0.1 298,785 + ABZR86_RS19215 0.84 -0.2 298,785 + ABZR86_RS19215 0.84 -2.0 298,836 - -0.1 298,836 - -0.4 298,836 - +0.6 298,908 + -0.7 298,909 - -0.1 299,255 + ABZR86_RS19220 0.58 +0.3 299,255 + ABZR86_RS19220 0.58 +0.0 299,256 - ABZR86_RS19220 0.58 +0.4 299,289 - ABZR86_RS19220 0.64 +0.7 299,357 + ABZR86_RS19220 0.78 +0.7 299,358 - ABZR86_RS19220 0.78 +0.1 299,358 - ABZR86_RS19220 0.78 -0.1 299,358 - ABZR86_RS19220 0.78 +0.2 299,399 + ABZR86_RS19220 0.86 +0.3 299,400 - ABZR86_RS19220 0.86 -0.3 299,495 + +0.2 299,534 + +0.2 299,534 + +0.2 299,534 + -0.8 299,535 - -0.2 299,535 - -0.8 299,535 - +0.3 299,709 + ABZR86_RS19225 0.21 -0.6 299,773 - ABZR86_RS19225 0.28 +0.3 299,773 - ABZR86_RS19225 0.28 -0.2 299,776 - ABZR86_RS19225 0.29 +0.1 299,955 + ABZR86_RS19225 0.48 -0.8 299,956 - ABZR86_RS19225 0.48 -0.1 299,956 - ABZR86_RS19225 0.48 +1.1 299,956 - ABZR86_RS19225 0.48 -1.1 300,103 - ABZR86_RS19225 0.65 +0.0 300,363 + -1.8 300,364 - -0.7 300,364 - -0.1 300,364 - -1.0 300,365 + -0.8 300,366 - -1.7
Or see this region's nucleotide sequence