Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_881 and Pf1N1B4_882 overlap by 4 nucleotides Pf1N1B4_882 and Pf1N1B4_883 are separated by 56 nucleotides Pf1N1B4_883 and Pf1N1B4_884 are separated by 146 nucleotides Pf1N1B4_884 and Pf1N1B4_885 are separated by 65 nucleotides
Pf1N1B4_881 - FIG00954164: hypothetical protein, at 1,006,441 to 1,006,656
_881
Pf1N1B4_882 - carbonic anhydrase, family 3, at 1,006,653 to 1,007,177
_882
Pf1N1B4_883 - Hypothetical nudix hydrolase YeaB, at 1,007,234 to 1,007,833
_883
Pf1N1B4_884 - MutT/nudix family protein, at 1,007,980 to 1,008,531
_884
Pf1N1B4_885 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 1,008,597 to 1,009,100
_885
Position (kb)
1007
1008 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1006.241 kb on + strand at 1006.241 kb on + strand at 1006.241 kb on + strand at 1006.241 kb on + strand at 1006.242 kb on - strand at 1006.242 kb on - strand at 1006.242 kb on - strand at 1006.266 kb on + strand at 1006.267 kb on - strand at 1006.284 kb on + strand at 1006.284 kb on + strand at 1006.284 kb on + strand at 1006.285 kb on - strand at 1006.285 kb on - strand at 1006.285 kb on - strand at 1006.285 kb on - strand at 1006.288 kb on + strand at 1006.288 kb on + strand at 1006.363 kb on + strand at 1006.433 kb on + strand at 1006.434 kb on - strand at 1006.705 kb on + strand at 1006.705 kb on + strand at 1006.705 kb on + strand at 1006.706 kb on - strand, within Pf1N1B4_882 at 1006.706 kb on - strand, within Pf1N1B4_882 at 1006.706 kb on - strand, within Pf1N1B4_882 at 1006.706 kb on - strand, within Pf1N1B4_882 at 1006.706 kb on - strand, within Pf1N1B4_882 at 1006.886 kb on + strand, within Pf1N1B4_882 at 1006.913 kb on + strand, within Pf1N1B4_882 at 1006.914 kb on - strand, within Pf1N1B4_882 at 1006.914 kb on - strand, within Pf1N1B4_882 at 1007.171 kb on - strand at 1007.171 kb on - strand at 1007.180 kb on + strand at 1007.180 kb on + strand at 1007.180 kb on + strand at 1007.181 kb on - strand at 1007.181 kb on - strand at 1007.245 kb on + strand at 1007.246 kb on - strand at 1007.246 kb on - strand at 1007.246 kb on - strand at 1007.286 kb on + strand at 1007.287 kb on - strand at 1007.287 kb on - strand at 1007.341 kb on - strand, within Pf1N1B4_883 at 1007.509 kb on - strand, within Pf1N1B4_883 at 1007.509 kb on - strand, within Pf1N1B4_883 at 1007.683 kb on + strand, within Pf1N1B4_883 at 1007.819 kb on + strand at 1007.819 kb on + strand at 1007.819 kb on + strand at 1007.819 kb on + strand at 1007.820 kb on - strand at 1007.820 kb on - strand at 1007.955 kb on - strand at 1007.955 kb on - strand at 1008.069 kb on + strand, within Pf1N1B4_884 at 1008.069 kb on + strand at 1008.069 kb on + strand, within Pf1N1B4_884 at 1008.069 kb on + strand, within Pf1N1B4_884 at 1008.070 kb on - strand, within Pf1N1B4_884 at 1008.080 kb on - strand, within Pf1N1B4_884 at 1008.094 kb on - strand, within Pf1N1B4_884 at 1008.273 kb on + strand, within Pf1N1B4_884 at 1008.273 kb on + strand at 1008.273 kb on + strand, within Pf1N1B4_884 at 1008.274 kb on - strand, within Pf1N1B4_884 at 1008.274 kb on - strand, within Pf1N1B4_884 at 1008.274 kb on - strand, within Pf1N1B4_884 at 1008.274 kb on - strand, within Pf1N1B4_884 at 1008.274 kb on - strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.277 kb on + strand, within Pf1N1B4_884 at 1008.278 kb on - strand, within Pf1N1B4_884 at 1008.278 kb on - strand, within Pf1N1B4_884 at 1008.278 kb on - strand, within Pf1N1B4_884 at 1008.278 kb on - strand, within Pf1N1B4_884 at 1008.278 kb on - strand, within Pf1N1B4_884 at 1008.279 kb on + strand, within Pf1N1B4_884 at 1008.279 kb on + strand, within Pf1N1B4_884 at 1008.279 kb on + strand, within Pf1N1B4_884 at 1008.438 kb on + strand, within Pf1N1B4_884 at 1008.443 kb on + strand, within Pf1N1B4_884 at 1008.443 kb on + strand, within Pf1N1B4_884 at 1008.443 kb on + strand, within Pf1N1B4_884 at 1008.443 kb on + strand, within Pf1N1B4_884 at 1008.443 kb on + strand, within Pf1N1B4_884 at 1008.443 kb on + strand, within Pf1N1B4_884 at 1008.443 kb on + strand, within Pf1N1B4_884 at 1008.444 kb on - strand, within Pf1N1B4_884 at 1008.444 kb on - strand, within Pf1N1B4_884 at 1008.444 kb on - strand, within Pf1N1B4_884 at 1008.444 kb on - strand, within Pf1N1B4_884 at 1008.444 kb on - strand, within Pf1N1B4_884 at 1008.478 kb on + strand at 1008.529 kb on + strand at 1008.529 kb on + strand at 1008.529 kb on + strand at 1008.530 kb on - strand at 1008.530 kb on - strand at 1008.530 kb on - strand at 1008.538 kb on + strand at 1008.539 kb on - strand at 1008.539 kb on - strand at 1008.544 kb on + strand at 1008.544 kb on + strand at 1008.544 kb on + strand at 1008.544 kb on + strand at 1008.544 kb on + strand at 1008.544 kb on + strand at 1008.544 kb on + strand at 1008.544 kb on + strand at 1008.544 kb on + strand at 1008.545 kb on - strand at 1008.545 kb on - strand at 1008.545 kb on - strand at 1008.545 kb on - strand at 1008.545 kb on - strand at 1008.545 kb on - strand at 1008.554 kb on + strand at 1008.554 kb on + strand at 1008.555 kb on - strand at 1008.555 kb on - strand at 1008.556 kb on + strand at 1008.561 kb on + strand at 1008.561 kb on + strand at 1008.561 kb on + strand at 1008.562 kb on - strand at 1008.562 kb on - strand at 1008.562 kb on - strand at 1008.828 kb on + strand, within Pf1N1B4_885 at 1008.828 kb on + strand, within Pf1N1B4_885 at 1008.828 kb on + strand, within Pf1N1B4_885 at 1008.828 kb on + strand, within Pf1N1B4_885 at 1008.828 kb on + strand, within Pf1N1B4_885 at 1008.828 kb on + strand, within Pf1N1B4_885
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 1,006,241 + -0.9 1,006,241 + -1.2 1,006,241 + -1.2 1,006,241 + -1.2 1,006,242 - +0.5 1,006,242 - +1.1 1,006,242 - +0.4 1,006,266 + -0.5 1,006,267 - -2.4 1,006,284 + -1.9 1,006,284 + -0.1 1,006,284 + -1.7 1,006,285 - +0.8 1,006,285 - -0.7 1,006,285 - +1.5 1,006,285 - -1.2 1,006,288 + -1.0 1,006,288 + -0.4 1,006,363 + +0.0 1,006,433 + +0.5 1,006,434 - -0.9 1,006,705 + +0.0 1,006,705 + -1.2 1,006,705 + +0.5 1,006,706 - Pf1N1B4_882 0.10 -0.2 1,006,706 - Pf1N1B4_882 0.10 +1.5 1,006,706 - Pf1N1B4_882 0.10 +2.4 1,006,706 - Pf1N1B4_882 0.10 -0.9 1,006,706 - Pf1N1B4_882 0.10 +1.3 1,006,886 + Pf1N1B4_882 0.44 -0.4 1,006,913 + Pf1N1B4_882 0.50 +1.4 1,006,914 - Pf1N1B4_882 0.50 +0.1 1,006,914 - Pf1N1B4_882 0.50 +0.5 1,007,171 - +1.1 1,007,171 - +0.1 1,007,180 + +0.8 1,007,180 + -0.2 1,007,180 + +0.1 1,007,181 - +1.7 1,007,181 - +0.1 1,007,245 + -0.8 1,007,246 - -2.1 1,007,246 - -0.2 1,007,246 - +0.9 1,007,286 + -0.5 1,007,287 - -0.9 1,007,287 - +0.1 1,007,341 - Pf1N1B4_883 0.18 -1.9 1,007,509 - Pf1N1B4_883 0.46 +1.7 1,007,509 - Pf1N1B4_883 0.46 -2.3 1,007,683 + Pf1N1B4_883 0.75 -0.3 1,007,819 + +0.1 1,007,819 + -1.2 1,007,819 + -0.0 1,007,819 + -0.9 1,007,820 - +1.9 1,007,820 - +0.1 1,007,955 - +0.1 1,007,955 - +1.1 1,008,069 + Pf1N1B4_884 0.16 -0.2 1,008,069 + -0.9 1,008,069 + Pf1N1B4_884 0.16 +0.8 1,008,069 + Pf1N1B4_884 0.16 -0.7 1,008,070 - Pf1N1B4_884 0.16 +0.1 1,008,080 - Pf1N1B4_884 0.18 +0.8 1,008,094 - Pf1N1B4_884 0.21 +0.0 1,008,273 + Pf1N1B4_884 0.53 +0.5 1,008,273 + -0.5 1,008,273 + Pf1N1B4_884 0.53 +0.1 1,008,274 - Pf1N1B4_884 0.53 +0.6 1,008,274 - Pf1N1B4_884 0.53 -0.2 1,008,274 - Pf1N1B4_884 0.53 -0.2 1,008,274 - Pf1N1B4_884 0.53 -0.5 1,008,274 - Pf1N1B4_884 0.53 -1.2 1,008,277 + Pf1N1B4_884 0.54 -1.4 1,008,277 + Pf1N1B4_884 0.54 +0.1 1,008,277 + Pf1N1B4_884 0.54 -0.8 1,008,277 + Pf1N1B4_884 0.54 -1.5 1,008,277 + Pf1N1B4_884 0.54 +0.1 1,008,277 + Pf1N1B4_884 0.54 -0.9 1,008,277 + Pf1N1B4_884 0.54 +1.7 1,008,277 + Pf1N1B4_884 0.54 +0.1 1,008,277 + Pf1N1B4_884 0.54 -0.2 1,008,277 + Pf1N1B4_884 0.54 +0.1 1,008,278 - Pf1N1B4_884 0.54 +0.8 1,008,278 - Pf1N1B4_884 0.54 -0.2 1,008,278 - Pf1N1B4_884 0.54 -2.2 1,008,278 - Pf1N1B4_884 0.54 -0.0 1,008,278 - Pf1N1B4_884 0.54 +0.7 1,008,279 + Pf1N1B4_884 0.54 +0.9 1,008,279 + Pf1N1B4_884 0.54 +0.8 1,008,279 + Pf1N1B4_884 0.54 -0.2 1,008,438 + Pf1N1B4_884 0.83 -0.3 1,008,443 + Pf1N1B4_884 0.84 +1.4 1,008,443 + Pf1N1B4_884 0.84 -0.2 1,008,443 + Pf1N1B4_884 0.84 -0.5 1,008,443 + Pf1N1B4_884 0.84 -0.9 1,008,443 + Pf1N1B4_884 0.84 -2.2 1,008,443 + Pf1N1B4_884 0.84 -0.1 1,008,443 + Pf1N1B4_884 0.84 -0.3 1,008,444 - Pf1N1B4_884 0.84 -2.1 1,008,444 - Pf1N1B4_884 0.84 +1.1 1,008,444 - Pf1N1B4_884 0.84 -0.7 1,008,444 - Pf1N1B4_884 0.84 +1.7 1,008,444 - Pf1N1B4_884 0.84 +2.1 1,008,478 + +0.1 1,008,529 + +0.5 1,008,529 + -0.7 1,008,529 + -1.2 1,008,530 - +0.4 1,008,530 - -0.5 1,008,530 - -0.5 1,008,538 + +0.3 1,008,539 - +1.1 1,008,539 - +0.9 1,008,544 + -1.3 1,008,544 + -0.4 1,008,544 + +1.6 1,008,544 + +0.1 1,008,544 + +0.4 1,008,544 + -0.9 1,008,544 + +0.0 1,008,544 + -0.7 1,008,544 + -0.5 1,008,545 - -0.9 1,008,545 - +0.5 1,008,545 - -1.0 1,008,545 - +1.1 1,008,545 - -0.9 1,008,545 - +0.8 1,008,554 + +1.1 1,008,554 + +1.1 1,008,555 - +0.2 1,008,555 - -0.4 1,008,556 + -0.2 1,008,561 + -0.2 1,008,561 + -0.5 1,008,561 + -1.3 1,008,562 - -1.2 1,008,562 - +1.1 1,008,562 - +0.0 1,008,828 + Pf1N1B4_885 0.46 +0.1 1,008,828 + Pf1N1B4_885 0.46 +0.7 1,008,828 + Pf1N1B4_885 0.46 +0.1 1,008,828 + Pf1N1B4_885 0.46 +0.3 1,008,828 + Pf1N1B4_885 0.46 -0.3 1,008,828 + Pf1N1B4_885 0.46 +1.7
Or see this region's nucleotide sequence