Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_800

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_798 and Pf1N1B4_799 are separated by 47 nucleotidesPf1N1B4_799 and Pf1N1B4_800 are separated by 12 nucleotidesPf1N1B4_800 and Pf1N1B4_801 are separated by 54 nucleotides Pf1N1B4_798 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 911,657 to 912,598 _798 Pf1N1B4_799 - hypothetical protein, at 912,646 to 912,807 _799 Pf1N1B4_800 - OpgC protein, at 912,820 to 913,944 _800 Pf1N1B4_801 - D-serine/D-alanine/glycine transporter, at 913,999 to 915,420 _801 Position (kb) 912 913 914Strain fitness (log2 ratio) -2 -1 0 1 2at 911.862 kb on + strand, within Pf1N1B4_798at 911.862 kb on + strand, within Pf1N1B4_798at 911.862 kb on + strand, within Pf1N1B4_798at 911.862 kb on + strandat 911.862 kb on + strand, within Pf1N1B4_798at 911.863 kb on - strand, within Pf1N1B4_798at 911.863 kb on - strand, within Pf1N1B4_798at 911.863 kb on - strand, within Pf1N1B4_798at 911.907 kb on + strand, within Pf1N1B4_798at 911.907 kb on + strand, within Pf1N1B4_798at 911.908 kb on - strand, within Pf1N1B4_798at 911.944 kb on + strand, within Pf1N1B4_798at 911.945 kb on - strand, within Pf1N1B4_798at 912.021 kb on + strand, within Pf1N1B4_798at 912.022 kb on - strand, within Pf1N1B4_798at 912.186 kb on + strand, within Pf1N1B4_798at 912.186 kb on + strand, within Pf1N1B4_798at 912.186 kb on + strand, within Pf1N1B4_798at 912.187 kb on - strand, within Pf1N1B4_798at 912.187 kb on - strand, within Pf1N1B4_798at 912.187 kb on - strand, within Pf1N1B4_798at 912.216 kb on + strand, within Pf1N1B4_798at 912.217 kb on - strand, within Pf1N1B4_798at 912.417 kb on - strand, within Pf1N1B4_798at 912.625 kb on + strandat 912.625 kb on + strandat 912.625 kb on + strandat 912.625 kb on + strandat 912.625 kb on + strandat 912.625 kb on + strandat 912.625 kb on + strandat 912.625 kb on + strandat 912.626 kb on - strandat 912.626 kb on - strandat 912.626 kb on - strandat 912.626 kb on - strandat 912.626 kb on - strandat 912.626 kb on - strandat 912.626 kb on - strandat 912.711 kb on + strandat 912.714 kb on - strand, within Pf1N1B4_799at 912.736 kb on + strand, within Pf1N1B4_799at 912.736 kb on + strand, within Pf1N1B4_799at 912.792 kb on + strandat 912.928 kb on - strandat 913.066 kb on + strand, within Pf1N1B4_800at 913.066 kb on + strand, within Pf1N1B4_800at 913.066 kb on + strand, within Pf1N1B4_800at 913.066 kb on + strand, within Pf1N1B4_800at 913.066 kb on + strand, within Pf1N1B4_800at 913.066 kb on + strand, within Pf1N1B4_800at 913.066 kb on + strand, within Pf1N1B4_800at 913.066 kb on + strand, within Pf1N1B4_800at 913.067 kb on - strand, within Pf1N1B4_800at 913.067 kb on - strand, within Pf1N1B4_800at 913.067 kb on - strand, within Pf1N1B4_800at 913.067 kb on - strand, within Pf1N1B4_800at 913.077 kb on + strand, within Pf1N1B4_800at 913.078 kb on - strand, within Pf1N1B4_800at 913.078 kb on - strand, within Pf1N1B4_800at 913.168 kb on + strand, within Pf1N1B4_800at 913.168 kb on + strand, within Pf1N1B4_800at 913.254 kb on + strand, within Pf1N1B4_800at 913.254 kb on + strand, within Pf1N1B4_800at 913.255 kb on - strand, within Pf1N1B4_800at 913.312 kb on + strand, within Pf1N1B4_800at 913.312 kb on + strand, within Pf1N1B4_800at 913.312 kb on + strand, within Pf1N1B4_800at 913.312 kb on + strand, within Pf1N1B4_800at 913.313 kb on - strand, within Pf1N1B4_800at 913.339 kb on + strand, within Pf1N1B4_800at 913.339 kb on + strand, within Pf1N1B4_800at 913.339 kb on + strand, within Pf1N1B4_800at 913.339 kb on + strand, within Pf1N1B4_800at 913.339 kb on + strandat 913.429 kb on - strand, within Pf1N1B4_800at 913.429 kb on - strand, within Pf1N1B4_800at 913.525 kb on + strand, within Pf1N1B4_800at 913.526 kb on - strand, within Pf1N1B4_800at 913.675 kb on + strand, within Pf1N1B4_800at 913.676 kb on - strand, within Pf1N1B4_800at 913.676 kb on - strand, within Pf1N1B4_800at 913.923 kb on + strandat 913.923 kb on + strandat 913.923 kb on + strandat 913.923 kb on + strandat 913.924 kb on - strandat 913.924 kb on - strandat 913.942 kb on + strandat 913.942 kb on + strandat 913.942 kb on + strandat 913.943 kb on - strandat 913.966 kb on - strandat 914.000 kb on + strandat 914.001 kb on - strandat 914.001 kb on - strandat 914.001 kb on - strandat 914.058 kb on - strandat 914.099 kb on + strandat 914.099 kb on + strandat 914.100 kb on - strandat 914.100 kb on - strandat 914.100 kb on - strandat 914.127 kb on - strandat 914.127 kb on - strandat 914.127 kb on - strandat 914.171 kb on + strand, within Pf1N1B4_801at 914.202 kb on - strandat 914.339 kb on + strand, within Pf1N1B4_801at 914.339 kb on + strand, within Pf1N1B4_801at 914.339 kb on + strand, within Pf1N1B4_801at 914.339 kb on + strand, within Pf1N1B4_801at 914.339 kb on + strandat 914.339 kb on + strand, within Pf1N1B4_801at 914.340 kb on - strand, within Pf1N1B4_801at 914.343 kb on + strand, within Pf1N1B4_801at 914.344 kb on - strand, within Pf1N1B4_801at 914.344 kb on - strand, within Pf1N1B4_801at 914.344 kb on - strand, within Pf1N1B4_801at 914.459 kb on + strand, within Pf1N1B4_801at 914.616 kb on - strand, within Pf1N1B4_801at 914.649 kb on - strand, within Pf1N1B4_801at 914.649 kb on - strand, within Pf1N1B4_801at 914.649 kb on - strand, within Pf1N1B4_801

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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911,862 + Pf1N1B4_798 0.22 -2.3
911,862 + Pf1N1B4_798 0.22 +0.8
911,862 + Pf1N1B4_798 0.22 -1.3
911,862 + +0.4
911,862 + Pf1N1B4_798 0.22 +0.3
911,863 - Pf1N1B4_798 0.22 +1.0
911,863 - Pf1N1B4_798 0.22 +1.8
911,863 - Pf1N1B4_798 0.22 +1.0
911,907 + Pf1N1B4_798 0.27 +0.4
911,907 + Pf1N1B4_798 0.27 +1.8
911,908 - Pf1N1B4_798 0.27 -0.7
911,944 + Pf1N1B4_798 0.30 +0.2
911,945 - Pf1N1B4_798 0.31 +1.0
912,021 + Pf1N1B4_798 0.39 +0.3
912,022 - Pf1N1B4_798 0.39 -2.0
912,186 + Pf1N1B4_798 0.56 +0.0
912,186 + Pf1N1B4_798 0.56 -1.1
912,186 + Pf1N1B4_798 0.56 -2.0
912,187 - Pf1N1B4_798 0.56 -0.8
912,187 - Pf1N1B4_798 0.56 -0.6
912,187 - Pf1N1B4_798 0.56 -1.4
912,216 + Pf1N1B4_798 0.59 -1.0
912,217 - Pf1N1B4_798 0.59 -0.2
912,417 - Pf1N1B4_798 0.81 -1.3
912,625 + -0.3
912,625 + +1.5
912,625 + -0.6
912,625 + +1.0
912,625 + +0.4
912,625 + -0.8
912,625 + +1.5
912,625 + -0.6
912,626 - +0.7
912,626 - +0.5
912,626 - +0.0
912,626 - -0.1
912,626 - -1.0
912,626 - +0.0
912,626 - -0.1
912,711 + +0.4
912,714 - Pf1N1B4_799 0.42 +0.4
912,736 + Pf1N1B4_799 0.56 +0.8
912,736 + Pf1N1B4_799 0.56 -0.1
912,792 + +0.0
912,928 - -2.1
913,066 + Pf1N1B4_800 0.22 +0.0
913,066 + Pf1N1B4_800 0.22 +0.3
913,066 + Pf1N1B4_800 0.22 -0.5
913,066 + Pf1N1B4_800 0.22 +1.0
913,066 + Pf1N1B4_800 0.22 -1.0
913,066 + Pf1N1B4_800 0.22 -0.8
913,066 + Pf1N1B4_800 0.22 -1.3
913,066 + Pf1N1B4_800 0.22 +0.7
913,067 - Pf1N1B4_800 0.22 +0.0
913,067 - Pf1N1B4_800 0.22 -1.3
913,067 - Pf1N1B4_800 0.22 -1.0
913,067 - Pf1N1B4_800 0.22 -2.4
913,077 + Pf1N1B4_800 0.23 -1.5
913,078 - Pf1N1B4_800 0.23 -1.0
913,078 - Pf1N1B4_800 0.23 -1.0
913,168 + Pf1N1B4_800 0.31 +0.2
913,168 + Pf1N1B4_800 0.31 +0.0
913,254 + Pf1N1B4_800 0.39 -0.5
913,254 + Pf1N1B4_800 0.39 -2.0
913,255 - Pf1N1B4_800 0.39 -1.3
913,312 + Pf1N1B4_800 0.44 -1.5
913,312 + Pf1N1B4_800 0.44 -0.2
913,312 + Pf1N1B4_800 0.44 -0.5
913,312 + Pf1N1B4_800 0.44 -2.5
913,313 - Pf1N1B4_800 0.44 -1.9
913,339 + Pf1N1B4_800 0.46 +0.3
913,339 + Pf1N1B4_800 0.46 +1.0
913,339 + Pf1N1B4_800 0.46 +0.0
913,339 + Pf1N1B4_800 0.46 +1.0
913,339 + +0.0
913,429 - Pf1N1B4_800 0.54 -0.5
913,429 - Pf1N1B4_800 0.54 -0.2
913,525 + Pf1N1B4_800 0.63 -2.0
913,526 - Pf1N1B4_800 0.63 -0.5
913,675 + Pf1N1B4_800 0.76 -1.8
913,676 - Pf1N1B4_800 0.76 -1.0
913,676 - Pf1N1B4_800 0.76 +0.5
913,923 + -0.6
913,923 + +0.0
913,923 + -0.5
913,923 + +0.3
913,924 - +0.7
913,924 - +0.5
913,942 + +0.6
913,942 + +0.0
913,942 + -1.3
913,943 - -1.5
913,966 - -0.3
914,000 + -0.4
914,001 - +1.0
914,001 - -0.5
914,001 - -1.3
914,058 - +0.3
914,099 + -0.2
914,099 + +0.5
914,100 - +1.0
914,100 - -0.3
914,100 - +0.0
914,127 - -1.3
914,127 - -1.3
914,127 - +0.5
914,171 + Pf1N1B4_801 0.12 -0.7
914,202 - -0.5
914,339 + Pf1N1B4_801 0.24 -0.8
914,339 + Pf1N1B4_801 0.24 -0.2
914,339 + Pf1N1B4_801 0.24 +0.0
914,339 + Pf1N1B4_801 0.24 -1.1
914,339 + +2.0
914,339 + Pf1N1B4_801 0.24 -1.0
914,340 - Pf1N1B4_801 0.24 -2.0
914,343 + Pf1N1B4_801 0.24 +1.0
914,344 - Pf1N1B4_801 0.24 -0.1
914,344 - Pf1N1B4_801 0.24 -1.1
914,344 - Pf1N1B4_801 0.24 +0.6
914,459 + Pf1N1B4_801 0.32 +0.6
914,616 - Pf1N1B4_801 0.43 -1.5
914,649 - Pf1N1B4_801 0.46 +0.0
914,649 - Pf1N1B4_801 0.46 -0.6
914,649 - Pf1N1B4_801 0.46 +0.7

Or see this region's nucleotide sequence