Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_765

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_764 and Pf1N1B4_765 are separated by 116 nucleotidesPf1N1B4_765 and Pf1N1B4_766 are separated by 413 nucleotides Pf1N1B4_764 - Uncharacterized isomerase yddE, PhzC-PhzF family, at 868,318 to 869,100 _764 Pf1N1B4_765 - Cys-tRNA(Pro) deacylase YbaK, at 869,217 to 869,687 _765 Pf1N1B4_766 - Glycerol uptake facilitator protein, at 870,101 to 870,910 _766 Position (kb) 869 870Strain fitness (log2 ratio) -2 -1 0 1 2 3at 868.249 kb on - strandat 868.274 kb on + strandat 868.275 kb on - strandat 868.275 kb on - strandat 868.275 kb on - strandat 868.330 kb on + strandat 868.331 kb on - strandat 868.331 kb on - strandat 868.331 kb on - strandat 868.331 kb on - strandat 868.427 kb on + strand, within Pf1N1B4_764at 868.428 kb on - strand, within Pf1N1B4_764at 868.654 kb on + strand, within Pf1N1B4_764at 868.655 kb on - strand, within Pf1N1B4_764at 868.844 kb on + strand, within Pf1N1B4_764at 868.844 kb on + strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.845 kb on - strand, within Pf1N1B4_764at 868.862 kb on + strand, within Pf1N1B4_764at 868.863 kb on - strand, within Pf1N1B4_764at 868.863 kb on - strand, within Pf1N1B4_764at 868.863 kb on - strand, within Pf1N1B4_764at 868.914 kb on + strand, within Pf1N1B4_764at 869.092 kb on + strandat 869.093 kb on - strandat 869.093 kb on - strandat 869.093 kb on - strandat 869.183 kb on + strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.184 kb on - strandat 869.355 kb on - strand, within Pf1N1B4_765at 869.355 kb on - strand, within Pf1N1B4_765at 869.602 kb on + strand, within Pf1N1B4_765at 869.602 kb on + strand, within Pf1N1B4_765at 869.602 kb on + strand, within Pf1N1B4_765at 869.602 kb on + strandat 869.629 kb on + strand, within Pf1N1B4_765at 869.629 kb on + strand, within Pf1N1B4_765at 869.736 kb on - strandat 869.751 kb on - strandat 869.763 kb on + strandat 869.763 kb on + strandat 869.763 kb on + strandat 869.763 kb on + strandat 869.763 kb on + strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.764 kb on - strandat 869.771 kb on + strandat 869.772 kb on - strandat 869.855 kb on + strandat 869.936 kb on + strandat 869.936 kb on + strandat 869.936 kb on + strandat 870.073 kb on - strandat 870.332 kb on + strand, within Pf1N1B4_766at 870.332 kb on + strandat 870.333 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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868,249 - +2.8
868,274 + +2.0
868,275 - -2.0
868,275 - -0.3
868,275 - +0.0
868,330 + +0.0
868,331 - -0.6
868,331 - -0.2
868,331 - -0.3
868,331 - -0.6
868,427 + Pf1N1B4_764 0.14 -0.8
868,428 - Pf1N1B4_764 0.14 -2.3
868,654 + Pf1N1B4_764 0.43 +0.4
868,655 - Pf1N1B4_764 0.43 -0.6
868,844 + Pf1N1B4_764 0.67 -1.0
868,844 + Pf1N1B4_764 0.67 -0.1
868,845 - Pf1N1B4_764 0.67 +0.7
868,845 - Pf1N1B4_764 0.67 -0.6
868,845 - Pf1N1B4_764 0.67 +1.0
868,845 - Pf1N1B4_764 0.67 -0.2
868,845 - Pf1N1B4_764 0.67 +2.0
868,845 - Pf1N1B4_764 0.67 -0.4
868,862 + Pf1N1B4_764 0.69 -0.6
868,863 - Pf1N1B4_764 0.70 -1.6
868,863 - Pf1N1B4_764 0.70 +0.4
868,863 - Pf1N1B4_764 0.70 +1.0
868,914 + Pf1N1B4_764 0.76 +2.0
869,092 + +0.8
869,093 - -0.2
869,093 - -0.3
869,093 - +0.6
869,183 + -0.6
869,184 - -0.8
869,184 - -1.3
869,184 - -0.1
869,184 - +1.6
869,184 - +0.4
869,184 - +1.0
869,355 - Pf1N1B4_765 0.29 -1.0
869,355 - Pf1N1B4_765 0.29 -2.0
869,602 + Pf1N1B4_765 0.82 +0.0
869,602 + Pf1N1B4_765 0.82 +1.4
869,602 + Pf1N1B4_765 0.82 -0.6
869,602 + +2.0
869,629 + Pf1N1B4_765 0.87 -0.3
869,629 + Pf1N1B4_765 0.87 -0.8
869,736 - +0.1
869,751 - -1.6
869,763 + -0.3
869,763 + +0.7
869,763 + -1.0
869,763 + +0.6
869,763 + -0.6
869,764 - +0.7
869,764 - +0.5
869,764 - -0.6
869,764 - -1.0
869,764 - +0.0
869,764 - +0.6
869,764 - +1.6
869,771 + -0.3
869,772 - +0.9
869,855 + -1.8
869,936 + +1.8
869,936 + -0.7
869,936 + -0.8
870,073 - -1.0
870,332 + Pf1N1B4_766 0.29 -1.0
870,332 + +0.0
870,333 - +0.4

Or see this region's nucleotide sequence