Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_724

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_723 and Pf1N1B4_724 overlap by 11 nucleotidesPf1N1B4_724 and Pf1N1B4_725 are separated by 218 nucleotides Pf1N1B4_723 - two-component system sensor protein, at 826,276 to 827,556 _723 Pf1N1B4_724 - DNA-binding response regulator ColR, at 827,546 to 828,229 _724 Pf1N1B4_725 - PAP2 superfamily protein, at 828,448 to 829,191 _725 Position (kb) 827 828 829Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 826.551 kb on + strand, within Pf1N1B4_723at 826.551 kb on + strand, within Pf1N1B4_723at 826.594 kb on - strand, within Pf1N1B4_723at 826.594 kb on - strand, within Pf1N1B4_723at 826.596 kb on - strand, within Pf1N1B4_723at 826.675 kb on + strand, within Pf1N1B4_723at 826.888 kb on + strand, within Pf1N1B4_723at 826.888 kb on + strand, within Pf1N1B4_723at 826.888 kb on + strand, within Pf1N1B4_723at 826.889 kb on - strand, within Pf1N1B4_723at 826.889 kb on - strand, within Pf1N1B4_723at 826.889 kb on - strand, within Pf1N1B4_723at 826.889 kb on - strand, within Pf1N1B4_723at 827.000 kb on + strand, within Pf1N1B4_723at 827.000 kb on + strand, within Pf1N1B4_723at 827.000 kb on + strand, within Pf1N1B4_723at 827.000 kb on + strand, within Pf1N1B4_723at 827.000 kb on + strand, within Pf1N1B4_723at 827.000 kb on + strand, within Pf1N1B4_723at 827.001 kb on - strand, within Pf1N1B4_723at 827.001 kb on - strand, within Pf1N1B4_723at 827.001 kb on - strand, within Pf1N1B4_723at 827.001 kb on - strand, within Pf1N1B4_723at 827.001 kb on - strand, within Pf1N1B4_723at 827.001 kb on - strand, within Pf1N1B4_723at 827.001 kb on - strand, within Pf1N1B4_723at 827.001 kb on - strand, within Pf1N1B4_723at 827.029 kb on + strand, within Pf1N1B4_723at 827.113 kb on + strand, within Pf1N1B4_723at 827.114 kb on - strand, within Pf1N1B4_723at 827.135 kb on - strand, within Pf1N1B4_723at 827.135 kb on - strand, within Pf1N1B4_723at 827.153 kb on - strand, within Pf1N1B4_723at 827.210 kb on + strand, within Pf1N1B4_723at 827.211 kb on - strand, within Pf1N1B4_723at 827.246 kb on + strand, within Pf1N1B4_723at 827.247 kb on - strand, within Pf1N1B4_723at 827.247 kb on - strand, within Pf1N1B4_723at 827.247 kb on - strand, within Pf1N1B4_723at 827.308 kb on + strand, within Pf1N1B4_723at 827.309 kb on - strand, within Pf1N1B4_723at 827.339 kb on + strand, within Pf1N1B4_723at 827.340 kb on - strand, within Pf1N1B4_723at 827.340 kb on - strand, within Pf1N1B4_723at 827.340 kb on - strand, within Pf1N1B4_723at 827.340 kb on - strand, within Pf1N1B4_723at 827.340 kb on - strand, within Pf1N1B4_723at 827.340 kb on - strand, within Pf1N1B4_723at 827.547 kb on + strandat 827.548 kb on - strandat 827.723 kb on + strand, within Pf1N1B4_724at 827.724 kb on - strand, within Pf1N1B4_724at 827.724 kb on - strand, within Pf1N1B4_724at 827.724 kb on - strand, within Pf1N1B4_724at 827.724 kb on - strand, within Pf1N1B4_724at 827.724 kb on - strand, within Pf1N1B4_724at 827.827 kb on - strand, within Pf1N1B4_724at 827.934 kb on + strand, within Pf1N1B4_724at 828.174 kb on + strandat 828.244 kb on + strandat 828.252 kb on - strandat 828.276 kb on - strandat 828.316 kb on + strandat 828.317 kb on - strandat 828.317 kb on - strandat 828.497 kb on + strandat 828.583 kb on + strand, within Pf1N1B4_725at 828.583 kb on + strand, within Pf1N1B4_725at 828.584 kb on - strandat 828.622 kb on + strand, within Pf1N1B4_725at 828.622 kb on + strand, within Pf1N1B4_725at 828.623 kb on - strand, within Pf1N1B4_725at 828.623 kb on - strand, within Pf1N1B4_725at 828.859 kb on + strand, within Pf1N1B4_725at 828.860 kb on - strand, within Pf1N1B4_725at 828.860 kb on - strand, within Pf1N1B4_725at 828.963 kb on - strand, within Pf1N1B4_725at 829.120 kb on + strandat 829.120 kb on + strandat 829.121 kb on - strandat 829.123 kb on + strandat 829.123 kb on + strandat 829.123 kb on + strandat 829.123 kb on + strandat 829.123 kb on + strandat 829.123 kb on + strandat 829.123 kb on + strandat 829.123 kb on + strandat 829.124 kb on - strandat 829.124 kb on - strandat 829.124 kb on - strandat 829.124 kb on - strandat 829.124 kb on - strandat 829.126 kb on + strandat 829.126 kb on + strandat 829.126 kb on + strandat 829.126 kb on + strandat 829.126 kb on + strandat 829.126 kb on + strandat 829.126 kb on + strandat 829.126 kb on + strandat 829.127 kb on - strandat 829.127 kb on - strandat 829.127 kb on - strandat 829.127 kb on - strandat 829.127 kb on - strandat 829.160 kb on + strandat 829.160 kb on + strandat 829.161 kb on - strandat 829.161 kb on - strandat 829.161 kb on - strandat 829.192 kb on + strandat 829.193 kb on - strandat 829.193 kb on - strandat 829.202 kb on + strandat 829.202 kb on + strandat 829.203 kb on - strandat 829.203 kb on - strandat 829.203 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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826,551 + Pf1N1B4_723 0.21 +0.1
826,551 + Pf1N1B4_723 0.21 +1.0
826,594 - Pf1N1B4_723 0.25 -0.0
826,594 - Pf1N1B4_723 0.25 +0.6
826,596 - Pf1N1B4_723 0.25 -0.6
826,675 + Pf1N1B4_723 0.31 -1.3
826,888 + Pf1N1B4_723 0.48 -0.0
826,888 + Pf1N1B4_723 0.48 +1.0
826,888 + Pf1N1B4_723 0.48 +0.9
826,889 - Pf1N1B4_723 0.48 +0.3
826,889 - Pf1N1B4_723 0.48 +0.2
826,889 - Pf1N1B4_723 0.48 +0.5
826,889 - Pf1N1B4_723 0.48 +1.8
827,000 + Pf1N1B4_723 0.57 -0.0
827,000 + Pf1N1B4_723 0.57 +2.8
827,000 + Pf1N1B4_723 0.57 -0.7
827,000 + Pf1N1B4_723 0.57 +1.2
827,000 + Pf1N1B4_723 0.57 +0.1
827,000 + Pf1N1B4_723 0.57 +0.6
827,001 - Pf1N1B4_723 0.57 -0.9
827,001 - Pf1N1B4_723 0.57 +2.6
827,001 - Pf1N1B4_723 0.57 +0.8
827,001 - Pf1N1B4_723 0.57 +0.7
827,001 - Pf1N1B4_723 0.57 +1.5
827,001 - Pf1N1B4_723 0.57 +2.3
827,001 - Pf1N1B4_723 0.57 -0.2
827,001 - Pf1N1B4_723 0.57 +0.7
827,029 + Pf1N1B4_723 0.59 -0.0
827,113 + Pf1N1B4_723 0.65 -0.3
827,114 - Pf1N1B4_723 0.65 +0.9
827,135 - Pf1N1B4_723 0.67 +1.3
827,135 - Pf1N1B4_723 0.67 -0.0
827,153 - Pf1N1B4_723 0.68 +0.7
827,210 + Pf1N1B4_723 0.73 +1.0
827,211 - Pf1N1B4_723 0.73 +0.9
827,246 + Pf1N1B4_723 0.76 -1.3
827,247 - Pf1N1B4_723 0.76 +0.9
827,247 - Pf1N1B4_723 0.76 -0.0
827,247 - Pf1N1B4_723 0.76 -1.1
827,308 + Pf1N1B4_723 0.81 +2.0
827,309 - Pf1N1B4_723 0.81 +1.3
827,339 + Pf1N1B4_723 0.83 -1.3
827,340 - Pf1N1B4_723 0.83 +0.4
827,340 - Pf1N1B4_723 0.83 +0.5
827,340 - Pf1N1B4_723 0.83 -0.9
827,340 - Pf1N1B4_723 0.83 +0.7
827,340 - Pf1N1B4_723 0.83 +0.6
827,340 - Pf1N1B4_723 0.83 +1.8
827,547 + +0.5
827,548 - -0.6
827,723 + Pf1N1B4_724 0.26 +0.4
827,724 - Pf1N1B4_724 0.26 -1.0
827,724 - Pf1N1B4_724 0.26 -2.8
827,724 - Pf1N1B4_724 0.26 -3.5
827,724 - Pf1N1B4_724 0.26 -1.3
827,724 - Pf1N1B4_724 0.26 -0.3
827,827 - Pf1N1B4_724 0.41 -0.0
827,934 + Pf1N1B4_724 0.57 -1.7
828,174 + -1.0
828,244 + -0.7
828,252 - -0.0
828,276 - +3.8
828,316 + -0.4
828,317 - +0.3
828,317 - +0.2
828,497 + -1.2
828,583 + Pf1N1B4_725 0.18 +0.8
828,583 + Pf1N1B4_725 0.18 +0.3
828,584 - +1.6
828,622 + Pf1N1B4_725 0.23 -1.2
828,622 + Pf1N1B4_725 0.23 +0.3
828,623 - Pf1N1B4_725 0.24 -0.6
828,623 - Pf1N1B4_725 0.24 -0.6
828,859 + Pf1N1B4_725 0.55 +0.6
828,860 - Pf1N1B4_725 0.55 +1.3
828,860 - Pf1N1B4_725 0.55 +1.6
828,963 - Pf1N1B4_725 0.69 +0.1
829,120 + +1.0
829,120 + -0.2
829,121 - -0.3
829,123 + -1.0
829,123 + +0.6
829,123 + +0.3
829,123 + +1.0
829,123 + +0.4
829,123 + +0.1
829,123 + -0.7
829,123 + +1.0
829,124 - -0.0
829,124 - -0.1
829,124 - -0.3
829,124 - +1.7
829,124 - -0.0
829,126 + -0.3
829,126 + -0.6
829,126 + -0.8
829,126 + -0.0
829,126 + +1.0
829,126 + -2.0
829,126 + +0.5
829,126 + -2.8
829,127 - +2.4
829,127 - +3.3
829,127 - -1.2
829,127 - -0.6
829,127 - +1.0
829,160 + -2.0
829,160 + +1.0
829,161 - -0.6
829,161 - -0.4
829,161 - +1.5
829,192 + -0.6
829,193 - +1.1
829,193 - -0.0
829,202 + +2.8
829,202 + +1.0
829,203 - -1.8
829,203 - -0.0
829,203 - -0.5

Or see this region's nucleotide sequence