Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_669 and Pf1N1B4_670 overlap by 4 nucleotides Pf1N1B4_670 and Pf1N1B4_671 overlap by 14 nucleotides Pf1N1B4_671 and Pf1N1B4_672 overlap by 4 nucleotides
Pf1N1B4_669 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 765,346 to 766,071
_669
Pf1N1B4_670 - FIG01248689: hypothetical protein, at 766,068 to 767,045
_670
Pf1N1B4_671 - 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases, at 767,032 to 768,576
_671
Pf1N1B4_672 - FIG00955538: hypothetical protein, at 768,573 to 769,736
_672
Position (kb) 767
768
769 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 766.230 kb on + strand, within Pf1N1B4_670 at 766.397 kb on - strand, within Pf1N1B4_670 at 766.434 kb on + strand, within Pf1N1B4_670 at 766.434 kb on + strand, within Pf1N1B4_670 at 766.435 kb on - strand, within Pf1N1B4_670 at 766.435 kb on - strand at 766.435 kb on - strand, within Pf1N1B4_670 at 766.435 kb on - strand, within Pf1N1B4_670 at 766.435 kb on - strand, within Pf1N1B4_670 at 766.455 kb on + strand, within Pf1N1B4_670 at 766.456 kb on - strand, within Pf1N1B4_670 at 766.456 kb on - strand, within Pf1N1B4_670 at 766.456 kb on - strand, within Pf1N1B4_670 at 766.456 kb on - strand, within Pf1N1B4_670 at 766.456 kb on - strand, within Pf1N1B4_670 at 766.456 kb on - strand, within Pf1N1B4_670 at 766.500 kb on + strand, within Pf1N1B4_670 at 766.500 kb on + strand, within Pf1N1B4_670 at 766.500 kb on + strand, within Pf1N1B4_670 at 766.500 kb on + strand, within Pf1N1B4_670 at 766.500 kb on + strand, within Pf1N1B4_670 at 766.500 kb on + strand, within Pf1N1B4_670 at 766.501 kb on - strand, within Pf1N1B4_670 at 766.501 kb on - strand, within Pf1N1B4_670 at 766.501 kb on - strand, within Pf1N1B4_670 at 766.501 kb on - strand, within Pf1N1B4_670 at 766.573 kb on - strand, within Pf1N1B4_670 at 766.573 kb on - strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.575 kb on + strand, within Pf1N1B4_670 at 766.576 kb on - strand, within Pf1N1B4_670 at 766.576 kb on - strand, within Pf1N1B4_670 at 766.576 kb on - strand, within Pf1N1B4_670 at 766.576 kb on - strand, within Pf1N1B4_670 at 766.576 kb on - strand, within Pf1N1B4_670 at 766.576 kb on - strand, within Pf1N1B4_670 at 766.576 kb on - strand, within Pf1N1B4_670 at 766.576 kb on - strand, within Pf1N1B4_670 at 766.576 kb on - strand, within Pf1N1B4_670 at 766.659 kb on + strand, within Pf1N1B4_670 at 766.659 kb on + strand, within Pf1N1B4_670 at 766.660 kb on - strand, within Pf1N1B4_670 at 766.660 kb on - strand, within Pf1N1B4_670 at 766.660 kb on - strand, within Pf1N1B4_670 at 766.660 kb on - strand, within Pf1N1B4_670 at 766.701 kb on + strand, within Pf1N1B4_670 at 766.701 kb on + strand at 766.701 kb on + strand, within Pf1N1B4_670 at 766.701 kb on + strand, within Pf1N1B4_670 at 766.702 kb on - strand, within Pf1N1B4_670 at 766.702 kb on - strand at 766.702 kb on - strand, within Pf1N1B4_670 at 766.702 kb on - strand, within Pf1N1B4_670 at 766.702 kb on - strand, within Pf1N1B4_670 at 766.702 kb on - strand, within Pf1N1B4_670 at 766.702 kb on - strand, within Pf1N1B4_670 at 766.702 kb on - strand, within Pf1N1B4_670 at 766.927 kb on - strand, within Pf1N1B4_670 at 766.965 kb on + strand at 767.004 kb on + strand at 767.004 kb on + strand at 767.004 kb on + strand at 767.005 kb on - strand at 767.005 kb on - strand at 767.008 kb on + strand at 767.008 kb on + strand at 767.008 kb on + strand at 767.008 kb on + strand at 767.009 kb on - strand at 767.009 kb on - strand at 767.009 kb on - strand at 767.009 kb on - strand at 767.257 kb on + strand, within Pf1N1B4_671 at 767.257 kb on + strand, within Pf1N1B4_671 at 767.258 kb on - strand, within Pf1N1B4_671 at 767.258 kb on - strand, within Pf1N1B4_671 at 767.545 kb on + strand, within Pf1N1B4_671 at 767.545 kb on + strand, within Pf1N1B4_671 at 767.545 kb on + strand at 767.545 kb on + strand, within Pf1N1B4_671 at 767.545 kb on + strand, within Pf1N1B4_671 at 767.545 kb on + strand, within Pf1N1B4_671 at 767.545 kb on + strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.546 kb on - strand, within Pf1N1B4_671 at 767.547 kb on + strand, within Pf1N1B4_671 at 767.547 kb on + strand, within Pf1N1B4_671 at 767.547 kb on + strand, within Pf1N1B4_671 at 767.548 kb on - strand, within Pf1N1B4_671 at 767.548 kb on - strand, within Pf1N1B4_671 at 767.548 kb on - strand, within Pf1N1B4_671 at 767.548 kb on - strand, within Pf1N1B4_671 at 767.650 kb on + strand, within Pf1N1B4_671 at 767.650 kb on + strand, within Pf1N1B4_671 at 767.651 kb on - strand, within Pf1N1B4_671 at 767.651 kb on - strand, within Pf1N1B4_671 at 767.651 kb on - strand, within Pf1N1B4_671 at 767.683 kb on + strand, within Pf1N1B4_671 at 767.683 kb on + strand, within Pf1N1B4_671 at 767.684 kb on - strand at 767.684 kb on - strand, within Pf1N1B4_671 at 767.684 kb on - strand, within Pf1N1B4_671 at 767.684 kb on - strand, within Pf1N1B4_671 at 767.684 kb on - strand, within Pf1N1B4_671 at 767.684 kb on - strand, within Pf1N1B4_671 at 767.859 kb on + strand, within Pf1N1B4_671 at 767.860 kb on - strand, within Pf1N1B4_671 at 768.083 kb on + strand, within Pf1N1B4_671 at 768.619 kb on + strand at 768.620 kb on - strand at 768.639 kb on + strand at 768.640 kb on - strand at 768.640 kb on - strand at 768.645 kb on + strand at 768.714 kb on + strand at 769.051 kb on - strand, within Pf1N1B4_672 at 769.051 kb on - strand, within Pf1N1B4_672 at 769.077 kb on + strand, within Pf1N1B4_672 at 769.078 kb on - strand, within Pf1N1B4_672 at 769.078 kb on - strand, within Pf1N1B4_672 at 769.243 kb on - strand, within Pf1N1B4_672 at 769.267 kb on - strand, within Pf1N1B4_672 at 769.305 kb on - strand, within Pf1N1B4_672
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 766,230 + Pf1N1B4_670 0.17 -1.6 766,397 - Pf1N1B4_670 0.34 -1.9 766,434 + Pf1N1B4_670 0.37 -0.6 766,434 + Pf1N1B4_670 0.37 -1.0 766,435 - Pf1N1B4_670 0.38 -0.4 766,435 - -0.6 766,435 - Pf1N1B4_670 0.38 +1.0 766,435 - Pf1N1B4_670 0.38 +0.6 766,435 - Pf1N1B4_670 0.38 +1.0 766,455 + Pf1N1B4_670 0.40 +0.6 766,456 - Pf1N1B4_670 0.40 -0.6 766,456 - Pf1N1B4_670 0.40 +0.4 766,456 - Pf1N1B4_670 0.40 +1.0 766,456 - Pf1N1B4_670 0.40 -0.2 766,456 - Pf1N1B4_670 0.40 +0.8 766,456 - Pf1N1B4_670 0.40 +0.5 766,500 + Pf1N1B4_670 0.44 -0.6 766,500 + Pf1N1B4_670 0.44 -0.3 766,500 + Pf1N1B4_670 0.44 -2.6 766,500 + Pf1N1B4_670 0.44 -0.4 766,500 + Pf1N1B4_670 0.44 -0.0 766,500 + Pf1N1B4_670 0.44 +0.2 766,501 - Pf1N1B4_670 0.44 +0.2 766,501 - Pf1N1B4_670 0.44 +1.1 766,501 - Pf1N1B4_670 0.44 -0.3 766,501 - Pf1N1B4_670 0.44 -2.0 766,573 - Pf1N1B4_670 0.52 +0.5 766,573 - Pf1N1B4_670 0.52 +0.5 766,575 + Pf1N1B4_670 0.52 -0.8 766,575 + Pf1N1B4_670 0.52 +1.5 766,575 + Pf1N1B4_670 0.52 -0.5 766,575 + Pf1N1B4_670 0.52 +1.0 766,575 + Pf1N1B4_670 0.52 +0.1 766,575 + Pf1N1B4_670 0.52 -0.6 766,575 + Pf1N1B4_670 0.52 -0.6 766,575 + Pf1N1B4_670 0.52 +0.3 766,575 + Pf1N1B4_670 0.52 -1.6 766,575 + Pf1N1B4_670 0.52 +0.3 766,576 - Pf1N1B4_670 0.52 +0.5 766,576 - Pf1N1B4_670 0.52 -1.4 766,576 - Pf1N1B4_670 0.52 +0.7 766,576 - Pf1N1B4_670 0.52 +1.0 766,576 - Pf1N1B4_670 0.52 -0.0 766,576 - Pf1N1B4_670 0.52 -1.8 766,576 - Pf1N1B4_670 0.52 +0.8 766,576 - Pf1N1B4_670 0.52 -0.5 766,576 - Pf1N1B4_670 0.52 -2.0 766,659 + Pf1N1B4_670 0.60 -1.6 766,659 + Pf1N1B4_670 0.60 -1.9 766,660 - Pf1N1B4_670 0.61 +1.0 766,660 - Pf1N1B4_670 0.61 -1.2 766,660 - Pf1N1B4_670 0.61 -0.9 766,660 - Pf1N1B4_670 0.61 +1.4 766,701 + Pf1N1B4_670 0.65 +0.2 766,701 + +2.0 766,701 + Pf1N1B4_670 0.65 +0.5 766,701 + Pf1N1B4_670 0.65 -0.4 766,702 - Pf1N1B4_670 0.65 +0.4 766,702 - +0.2 766,702 - Pf1N1B4_670 0.65 -0.9 766,702 - Pf1N1B4_670 0.65 -1.4 766,702 - Pf1N1B4_670 0.65 -0.2 766,702 - Pf1N1B4_670 0.65 +0.2 766,702 - Pf1N1B4_670 0.65 +0.3 766,702 - Pf1N1B4_670 0.65 -0.0 766,927 - Pf1N1B4_670 0.88 -1.2 766,965 + -0.6 767,004 + -1.5 767,004 + +0.5 767,004 + -1.0 767,005 - -0.4 767,005 - +1.0 767,008 + +0.6 767,008 + -1.6 767,008 + -1.9 767,008 + -0.0 767,009 - -0.3 767,009 - -1.4 767,009 - -1.6 767,009 - +1.4 767,257 + Pf1N1B4_671 0.15 -0.2 767,257 + Pf1N1B4_671 0.15 +2.5 767,258 - Pf1N1B4_671 0.15 -0.6 767,258 - Pf1N1B4_671 0.15 -0.3 767,545 + Pf1N1B4_671 0.33 -2.7 767,545 + Pf1N1B4_671 0.33 +0.3 767,545 + +1.0 767,545 + Pf1N1B4_671 0.33 -0.2 767,545 + Pf1N1B4_671 0.33 -0.3 767,545 + Pf1N1B4_671 0.33 +0.4 767,545 + Pf1N1B4_671 0.33 -1.6 767,546 - Pf1N1B4_671 0.33 -0.6 767,546 - +1.0 767,546 - Pf1N1B4_671 0.33 +1.8 767,546 - Pf1N1B4_671 0.33 +1.0 767,546 - Pf1N1B4_671 0.33 -1.0 767,546 - Pf1N1B4_671 0.33 +0.4 767,546 - Pf1N1B4_671 0.33 -2.0 767,546 - Pf1N1B4_671 0.33 +0.4 767,546 - Pf1N1B4_671 0.33 +0.6 767,546 - Pf1N1B4_671 0.33 -1.0 767,546 - Pf1N1B4_671 0.33 -0.2 767,546 - Pf1N1B4_671 0.33 -0.0 767,547 + Pf1N1B4_671 0.33 -1.4 767,547 + Pf1N1B4_671 0.33 -1.0 767,547 + Pf1N1B4_671 0.33 +0.1 767,548 - Pf1N1B4_671 0.33 +1.0 767,548 - Pf1N1B4_671 0.33 -0.1 767,548 - Pf1N1B4_671 0.33 +0.9 767,548 - Pf1N1B4_671 0.33 +2.0 767,650 + Pf1N1B4_671 0.40 +0.4 767,650 + Pf1N1B4_671 0.40 -0.6 767,651 - Pf1N1B4_671 0.40 +0.5 767,651 - Pf1N1B4_671 0.40 -0.6 767,651 - Pf1N1B4_671 0.40 +0.8 767,683 + Pf1N1B4_671 0.42 -1.9 767,683 + Pf1N1B4_671 0.42 -0.0 767,684 - -0.0 767,684 - Pf1N1B4_671 0.42 -1.6 767,684 - Pf1N1B4_671 0.42 -0.4 767,684 - Pf1N1B4_671 0.42 +1.0 767,684 - Pf1N1B4_671 0.42 +1.0 767,684 - Pf1N1B4_671 0.42 -0.6 767,859 + Pf1N1B4_671 0.54 +0.2 767,860 - Pf1N1B4_671 0.54 +0.2 768,083 + Pf1N1B4_671 0.68 -3.0 768,619 + +0.8 768,620 - -0.5 768,639 + -0.6 768,640 - +0.2 768,640 - -2.2 768,645 + +0.4 768,714 + +1.0 769,051 - Pf1N1B4_672 0.41 +1.0 769,051 - Pf1N1B4_672 0.41 -0.4 769,077 + Pf1N1B4_672 0.43 -1.0 769,078 - Pf1N1B4_672 0.43 -0.0 769,078 - Pf1N1B4_672 0.43 -1.4 769,243 - Pf1N1B4_672 0.58 -1.0 769,267 - Pf1N1B4_672 0.60 -0.6 769,305 - Pf1N1B4_672 0.63 +0.2
Or see this region's nucleotide sequence