Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_660 and Pf1N1B4_661 are separated by 219 nucleotides Pf1N1B4_661 and Pf1N1B4_662 are separated by 276 nucleotides Pf1N1B4_662 and Pf1N1B4_663 overlap by 4 nucleotides
Pf1N1B4_660 - Chemotactic transducer, at 756,803 to 758,692
_660
Pf1N1B4_661 - glycosyl transferase, group 1 family protein, at 758,912 to 760,033
_661
Pf1N1B4_662 - FIG00955875: hypothetical protein, at 760,310 to 760,801
_662
Pf1N1B4_663 - FIG00955324: hypothetical protein, at 760,798 to 761,793
_663
Position (kb)
758
759
760
761 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 757.957 kb on + strand, within Pf1N1B4_660 at 757.957 kb on + strand, within Pf1N1B4_660 at 758.582 kb on - strand at 758.582 kb on - strand at 758.582 kb on - strand at 758.677 kb on + strand at 758.677 kb on + strand at 758.677 kb on + strand at 758.677 kb on + strand at 758.755 kb on + strand at 758.755 kb on + strand at 758.755 kb on + strand at 758.755 kb on + strand at 758.755 kb on + strand at 758.755 kb on + strand at 758.756 kb on - strand at 758.756 kb on - strand at 758.756 kb on - strand at 758.865 kb on + strand at 758.865 kb on + strand at 758.865 kb on + strand at 758.866 kb on - strand at 758.866 kb on - strand at 758.866 kb on - strand at 758.866 kb on - strand at 758.866 kb on - strand at 758.866 kb on - strand at 758.866 kb on - strand at 758.866 kb on - strand at 758.866 kb on - strand at 758.874 kb on + strand at 758.875 kb on - strand at 758.875 kb on - strand at 758.876 kb on + strand at 758.876 kb on + strand at 758.876 kb on + strand at 758.876 kb on + strand at 758.876 kb on + strand at 758.876 kb on + strand at 758.876 kb on + strand at 758.876 kb on + strand at 758.876 kb on + strand at 758.876 kb on + strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.877 kb on - strand at 758.937 kb on + strand at 758.937 kb on + strand at 758.937 kb on + strand at 758.937 kb on + strand at 758.937 kb on + strand at 758.938 kb on - strand at 758.938 kb on - strand at 758.938 kb on - strand at 758.938 kb on - strand at 758.938 kb on - strand at 758.938 kb on - strand at 758.938 kb on - strand at 758.938 kb on - strand at 758.938 kb on - strand at 758.948 kb on + strand at 758.949 kb on - strand at 758.949 kb on - strand at 758.978 kb on + strand at 758.978 kb on + strand at 758.979 kb on - strand at 758.979 kb on - strand at 758.979 kb on - strand at 759.194 kb on + strand, within Pf1N1B4_661 at 759.257 kb on + strand, within Pf1N1B4_661 at 759.307 kb on + strand, within Pf1N1B4_661 at 759.326 kb on + strand, within Pf1N1B4_661 at 759.326 kb on + strand, within Pf1N1B4_661 at 759.326 kb on + strand, within Pf1N1B4_661 at 759.327 kb on - strand at 759.327 kb on - strand, within Pf1N1B4_661 at 759.327 kb on - strand, within Pf1N1B4_661 at 759.327 kb on - strand, within Pf1N1B4_661 at 759.327 kb on - strand, within Pf1N1B4_661 at 759.327 kb on - strand, within Pf1N1B4_661 at 759.327 kb on - strand, within Pf1N1B4_661 at 759.341 kb on + strand, within Pf1N1B4_661 at 759.341 kb on + strand, within Pf1N1B4_661 at 759.342 kb on - strand, within Pf1N1B4_661 at 759.342 kb on - strand, within Pf1N1B4_661 at 759.342 kb on - strand, within Pf1N1B4_661 at 759.342 kb on - strand, within Pf1N1B4_661 at 759.343 kb on + strand, within Pf1N1B4_661 at 759.343 kb on + strand, within Pf1N1B4_661 at 759.343 kb on + strand, within Pf1N1B4_661 at 759.343 kb on + strand, within Pf1N1B4_661 at 759.344 kb on - strand, within Pf1N1B4_661 at 759.344 kb on - strand, within Pf1N1B4_661 at 759.389 kb on - strand, within Pf1N1B4_661 at 759.424 kb on - strand, within Pf1N1B4_661 at 759.508 kb on + strand, within Pf1N1B4_661 at 759.509 kb on - strand, within Pf1N1B4_661 at 759.607 kb on - strand at 759.947 kb on + strand at 759.947 kb on + strand at 759.947 kb on + strand at 759.948 kb on - strand at 759.948 kb on - strand at 759.987 kb on - strand at 760.178 kb on + strand at 760.178 kb on + strand at 760.179 kb on - strand at 760.260 kb on + strand at 760.323 kb on - strand at 760.323 kb on - strand at 760.323 kb on - strand at 760.343 kb on + strand at 760.407 kb on - strand, within Pf1N1B4_662 at 760.407 kb on - strand, within Pf1N1B4_662 at 760.408 kb on + strand, within Pf1N1B4_662 at 760.408 kb on + strand, within Pf1N1B4_662 at 760.408 kb on + strand, within Pf1N1B4_662 at 760.408 kb on + strand, within Pf1N1B4_662 at 760.409 kb on - strand at 760.409 kb on - strand, within Pf1N1B4_662 at 760.409 kb on - strand, within Pf1N1B4_662 at 760.409 kb on - strand at 760.409 kb on - strand, within Pf1N1B4_662
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 757,957 + Pf1N1B4_660 0.61 +1.4 757,957 + Pf1N1B4_660 0.61 -0.1 758,582 - +0.2 758,582 - +1.4 758,582 - -0.4 758,677 + -0.6 758,677 + -0.8 758,677 + -0.6 758,677 + -0.1 758,755 + -2.1 758,755 + -0.6 758,755 + -0.4 758,755 + -0.1 758,755 + -1.1 758,755 + +0.1 758,756 - +0.8 758,756 - +0.6 758,756 - -0.4 758,865 + +0.5 758,865 + +1.1 758,865 + +1.2 758,866 - -0.2 758,866 - +0.3 758,866 - +0.9 758,866 - -0.5 758,866 - -1.1 758,866 - +0.9 758,866 - +1.2 758,866 - -1.4 758,866 - -0.6 758,874 + +0.1 758,875 - -0.6 758,875 - -0.5 758,876 + -0.1 758,876 + -0.6 758,876 + +0.1 758,876 + +0.3 758,876 + +0.9 758,876 + +0.2 758,876 + +0.9 758,876 + -0.1 758,876 + +0.6 758,876 + -1.1 758,877 - +0.8 758,877 - +0.9 758,877 - +0.7 758,877 - -1.6 758,877 - -0.6 758,877 - +0.2 758,877 - -1.1 758,877 - +1.7 758,877 - +0.1 758,877 - +0.4 758,877 - -0.4 758,877 - +0.9 758,877 - -0.1 758,877 - +0.3 758,877 - -1.4 758,877 - +0.1 758,937 + +0.6 758,937 + -1.6 758,937 + +1.0 758,937 + -0.4 758,937 + -1.9 758,938 - -1.6 758,938 - +0.9 758,938 - +0.1 758,938 - +0.5 758,938 - +0.2 758,938 - +0.2 758,938 - -0.2 758,938 - +0.8 758,938 - +2.9 758,948 + -0.6 758,949 - -0.9 758,949 - -2.2 758,978 + -0.5 758,978 + +0.4 758,979 - +1.9 758,979 - +0.2 758,979 - -0.1 759,194 + Pf1N1B4_661 0.25 -2.1 759,257 + Pf1N1B4_661 0.31 +0.9 759,307 + Pf1N1B4_661 0.35 +0.9 759,326 + Pf1N1B4_661 0.37 -1.3 759,326 + Pf1N1B4_661 0.37 -1.9 759,326 + Pf1N1B4_661 0.37 -1.9 759,327 - -0.1 759,327 - Pf1N1B4_661 0.37 -1.4 759,327 - Pf1N1B4_661 0.37 -0.6 759,327 - Pf1N1B4_661 0.37 -0.6 759,327 - Pf1N1B4_661 0.37 -1.4 759,327 - Pf1N1B4_661 0.37 +0.4 759,327 - Pf1N1B4_661 0.37 +0.9 759,341 + Pf1N1B4_661 0.38 +0.9 759,341 + Pf1N1B4_661 0.38 +0.6 759,342 - Pf1N1B4_661 0.38 +0.9 759,342 - Pf1N1B4_661 0.38 -1.6 759,342 - Pf1N1B4_661 0.38 -1.6 759,342 - Pf1N1B4_661 0.38 -0.6 759,343 + Pf1N1B4_661 0.38 -1.0 759,343 + Pf1N1B4_661 0.38 -0.1 759,343 + Pf1N1B4_661 0.38 +0.4 759,343 + Pf1N1B4_661 0.38 +1.2 759,344 - Pf1N1B4_661 0.39 -1.7 759,344 - Pf1N1B4_661 0.39 +1.9 759,389 - Pf1N1B4_661 0.43 +2.8 759,424 - Pf1N1B4_661 0.46 -0.7 759,508 + Pf1N1B4_661 0.53 -0.2 759,509 - Pf1N1B4_661 0.53 -0.3 759,607 - -0.1 759,947 + +0.5 759,947 + -0.1 759,947 + +0.1 759,948 - +1.9 759,948 - -0.3 759,987 - +0.4 760,178 + +1.4 760,178 + +0.7 760,179 - +3.5 760,260 + +0.4 760,323 - -1.1 760,323 - -0.7 760,323 - -0.1 760,343 + +0.9 760,407 - Pf1N1B4_662 0.20 +1.7 760,407 - Pf1N1B4_662 0.20 -0.5 760,408 + Pf1N1B4_662 0.20 -2.1 760,408 + Pf1N1B4_662 0.20 -0.1 760,408 + Pf1N1B4_662 0.20 -0.6 760,408 + Pf1N1B4_662 0.20 -0.1 760,409 - -0.1 760,409 - Pf1N1B4_662 0.20 -0.5 760,409 - Pf1N1B4_662 0.20 +1.9 760,409 - -0.6 760,409 - Pf1N1B4_662 0.20 +0.4
Or see this region's nucleotide sequence