Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_606

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_603 and Pf1N1B4_604 are separated by 77 nucleotidesPf1N1B4_604 and Pf1N1B4_605 are separated by 28 nucleotidesPf1N1B4_605 and Pf1N1B4_606 overlap by 69 nucleotidesPf1N1B4_606 and Pf1N1B4_607 are separated by 122 nucleotides Pf1N1B4_603 - Methylglyoxal synthase (EC 4.2.3.3), at 697,008 to 697,472 _603 Pf1N1B4_604 - FIG006045: Sigma factor, ECF subfamily, at 697,550 to 698,068 _604 Pf1N1B4_605 - hypothetical protein, at 698,097 to 698,288 _605 Pf1N1B4_606 - Iron siderophore sensor protein, at 698,220 to 699,128 _606 Pf1N1B4_607 - TonB-dependent hemin , ferrichrome receptor, at 699,251 to 701,812 _607 Position (kb) 698 699 700Strain fitness (log2 ratio) -2 -1 0 1 2at 697.389 kb on + strand, within Pf1N1B4_603at 697.389 kb on + strand, within Pf1N1B4_603at 697.389 kb on + strand, within Pf1N1B4_603at 697.390 kb on - strand, within Pf1N1B4_603at 697.390 kb on - strand, within Pf1N1B4_603at 697.400 kb on + strand, within Pf1N1B4_603at 697.400 kb on + strand, within Pf1N1B4_603at 697.401 kb on - strand, within Pf1N1B4_603at 697.401 kb on - strand, within Pf1N1B4_603at 697.444 kb on - strandat 697.452 kb on + strandat 697.452 kb on + strandat 697.452 kb on + strandat 697.452 kb on + strandat 697.453 kb on - strandat 697.453 kb on - strandat 697.453 kb on - strandat 697.471 kb on - strandat 697.664 kb on + strand, within Pf1N1B4_604at 697.665 kb on - strand, within Pf1N1B4_604at 697.665 kb on - strand, within Pf1N1B4_604at 697.958 kb on + strand, within Pf1N1B4_604at 697.958 kb on + strand, within Pf1N1B4_604at 697.958 kb on + strand, within Pf1N1B4_604at 697.958 kb on + strand, within Pf1N1B4_604at 697.959 kb on - strand, within Pf1N1B4_604at 697.959 kb on - strand, within Pf1N1B4_604at 697.959 kb on - strand, within Pf1N1B4_604at 697.959 kb on - strand, within Pf1N1B4_604at 698.098 kb on + strandat 698.098 kb on + strandat 698.635 kb on - strand, within Pf1N1B4_606at 698.840 kb on + strand, within Pf1N1B4_606at 698.840 kb on + strand, within Pf1N1B4_606at 698.974 kb on - strand, within Pf1N1B4_606at 699.030 kb on - strand, within Pf1N1B4_606at 699.219 kb on + strandat 699.219 kb on + strandat 699.219 kb on + strandat 699.220 kb on - strandat 699.220 kb on - strandat 699.220 kb on - strandat 699.222 kb on - strandat 699.222 kb on - strandat 699.294 kb on + strandat 699.294 kb on + strandat 699.294 kb on + strandat 699.294 kb on + strandat 699.295 kb on - strandat 699.295 kb on - strandat 699.295 kb on - strandat 699.386 kb on + strandat 699.386 kb on + strandat 699.584 kb on - strand, within Pf1N1B4_607at 699.737 kb on - strandat 699.813 kb on - strand, within Pf1N1B4_607at 699.897 kb on - strand, within Pf1N1B4_607at 699.917 kb on + strand, within Pf1N1B4_607at 699.917 kb on + strand, within Pf1N1B4_607at 699.917 kb on + strand, within Pf1N1B4_607at 699.917 kb on + strand, within Pf1N1B4_607at 699.917 kb on + strand, within Pf1N1B4_607at 699.917 kb on + strand, within Pf1N1B4_607at 699.917 kb on + strand, within Pf1N1B4_607at 699.917 kb on + strand, within Pf1N1B4_607at 699.917 kb on + strandat 699.918 kb on - strandat 699.918 kb on - strandat 699.918 kb on - strand, within Pf1N1B4_607at 699.918 kb on - strand, within Pf1N1B4_607at 699.918 kb on - strand, within Pf1N1B4_607at 699.918 kb on - strand, within Pf1N1B4_607at 699.975 kb on + strand, within Pf1N1B4_607at 699.976 kb on - strand, within Pf1N1B4_607at 699.978 kb on - strand, within Pf1N1B4_607at 700.011 kb on - strand, within Pf1N1B4_607at 700.011 kb on - strand, within Pf1N1B4_607at 700.011 kb on - strand, within Pf1N1B4_607at 700.013 kb on + strand, within Pf1N1B4_607at 700.013 kb on + strand, within Pf1N1B4_607at 700.013 kb on + strand, within Pf1N1B4_607at 700.013 kb on + strand, within Pf1N1B4_607at 700.014 kb on - strand, within Pf1N1B4_607at 700.014 kb on - strand, within Pf1N1B4_607at 700.014 kb on - strand, within Pf1N1B4_607at 700.014 kb on - strand, within Pf1N1B4_607at 700.014 kb on - strand, within Pf1N1B4_607at 700.079 kb on + strand, within Pf1N1B4_607

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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697,389 + Pf1N1B4_603 0.82 -1.5
697,389 + Pf1N1B4_603 0.82 -0.4
697,389 + Pf1N1B4_603 0.82 -1.2
697,390 - Pf1N1B4_603 0.82 +0.3
697,390 - Pf1N1B4_603 0.82 -1.1
697,400 + Pf1N1B4_603 0.84 +0.9
697,400 + Pf1N1B4_603 0.84 -0.1
697,401 - Pf1N1B4_603 0.85 +0.2
697,401 - Pf1N1B4_603 0.85 -1.7
697,444 - +0.3
697,452 + -1.7
697,452 + -1.9
697,452 + +0.9
697,452 + -1.1
697,453 - -1.7
697,453 - +0.2
697,453 - -0.4
697,471 - -0.1
697,664 + Pf1N1B4_604 0.22 -0.6
697,665 - Pf1N1B4_604 0.22 +0.9
697,665 - Pf1N1B4_604 0.22 +0.1
697,958 + Pf1N1B4_604 0.79 -0.2
697,958 + Pf1N1B4_604 0.79 +1.5
697,958 + Pf1N1B4_604 0.79 -1.7
697,958 + Pf1N1B4_604 0.79 -0.7
697,959 - Pf1N1B4_604 0.79 +0.1
697,959 - Pf1N1B4_604 0.79 +0.2
697,959 - Pf1N1B4_604 0.79 -1.2
697,959 - Pf1N1B4_604 0.79 +0.1
698,098 + +0.2
698,098 + -0.7
698,635 - Pf1N1B4_606 0.46 -1.4
698,840 + Pf1N1B4_606 0.68 -1.1
698,840 + Pf1N1B4_606 0.68 -0.6
698,974 - Pf1N1B4_606 0.83 -0.4
699,030 - Pf1N1B4_606 0.89 -1.7
699,219 + -1.7
699,219 + -1.1
699,219 + -0.1
699,220 - -0.4
699,220 - -2.4
699,220 - -1.4
699,222 - +0.3
699,222 - +0.2
699,294 + -0.7
699,294 + -0.7
699,294 + -0.8
699,294 + -0.7
699,295 - +0.2
699,295 - -0.9
699,295 - +0.5
699,386 + -0.1
699,386 + +0.3
699,584 - Pf1N1B4_607 0.13 -0.5
699,737 - -1.7
699,813 - Pf1N1B4_607 0.22 -0.1
699,897 - Pf1N1B4_607 0.25 +0.2
699,917 + Pf1N1B4_607 0.26 +0.3
699,917 + Pf1N1B4_607 0.26 -1.4
699,917 + Pf1N1B4_607 0.26 -1.9
699,917 + Pf1N1B4_607 0.26 +0.9
699,917 + Pf1N1B4_607 0.26 -0.5
699,917 + Pf1N1B4_607 0.26 -1.4
699,917 + Pf1N1B4_607 0.26 +1.6
699,917 + Pf1N1B4_607 0.26 +0.1
699,917 + -1.4
699,918 - -0.1
699,918 - -0.1
699,918 - Pf1N1B4_607 0.26 -1.7
699,918 - Pf1N1B4_607 0.26 -0.7
699,918 - Pf1N1B4_607 0.26 -0.7
699,918 - Pf1N1B4_607 0.26 +1.9
699,975 + Pf1N1B4_607 0.28 +0.2
699,976 - Pf1N1B4_607 0.28 +0.5
699,978 - Pf1N1B4_607 0.28 -0.7
700,011 - Pf1N1B4_607 0.30 -0.5
700,011 - Pf1N1B4_607 0.30 -2.4
700,011 - Pf1N1B4_607 0.30 -0.3
700,013 + Pf1N1B4_607 0.30 +0.2
700,013 + Pf1N1B4_607 0.30 -0.4
700,013 + Pf1N1B4_607 0.30 -0.4
700,013 + Pf1N1B4_607 0.30 +0.1
700,014 - Pf1N1B4_607 0.30 -0.1
700,014 - Pf1N1B4_607 0.30 -0.9
700,014 - Pf1N1B4_607 0.30 +0.3
700,014 - Pf1N1B4_607 0.30 -1.5
700,014 - Pf1N1B4_607 0.30 -0.7
700,079 + Pf1N1B4_607 0.32 -0.1

Or see this region's nucleotide sequence