Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6004 and Pf1N1B4_6005 overlap by 4 nucleotides
Pf1N1B4_6004 - tgtA5 cluster protein 1, at 459,335 to 460,456
_6004
Pf1N1B4_6005 - archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 5, at 460,453 to 461,751
_6005
Position (kb) 459
460
461 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 458.339 kb on + strand at 458.346 kb on + strand at 458.484 kb on + strand at 458.857 kb on - strand at 458.963 kb on - strand at 459.245 kb on - strand at 459.322 kb on + strand at 459.370 kb on - strand at 459.401 kb on + strand at 459.401 kb on + strand at 459.401 kb on + strand at 459.401 kb on + strand at 459.402 kb on - strand at 459.402 kb on - strand at 459.402 kb on - strand at 459.408 kb on + strand at 459.534 kb on - strand, within Pf1N1B4_6004 at 459.571 kb on + strand, within Pf1N1B4_6004 at 459.814 kb on + strand, within Pf1N1B4_6004 at 459.814 kb on + strand, within Pf1N1B4_6004 at 459.814 kb on + strand, within Pf1N1B4_6004 at 460.029 kb on + strand at 460.082 kb on + strand, within Pf1N1B4_6004 at 460.124 kb on + strand, within Pf1N1B4_6004 at 460.124 kb on + strand, within Pf1N1B4_6004 at 460.124 kb on + strand, within Pf1N1B4_6004 at 460.125 kb on - strand, within Pf1N1B4_6004 at 460.131 kb on + strand, within Pf1N1B4_6004 at 460.131 kb on + strand, within Pf1N1B4_6004 at 460.132 kb on - strand at 460.162 kb on + strand, within Pf1N1B4_6004 at 460.256 kb on + strand, within Pf1N1B4_6004 at 460.256 kb on + strand, within Pf1N1B4_6004 at 460.299 kb on + strand, within Pf1N1B4_6004 at 460.360 kb on + strand at 460.399 kb on + strand at 460.400 kb on - strand at 460.433 kb on + strand at 460.433 kb on + strand at 460.435 kb on + strand at 460.768 kb on + strand, within Pf1N1B4_6005 at 460.772 kb on + strand, within Pf1N1B4_6005 at 461.093 kb on + strand, within Pf1N1B4_6005
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 458,339 + -0.7 458,346 + +0.0 458,484 + +0.9 458,857 - +0.5 458,963 - -0.8 459,245 - +0.5 459,322 + -2.5 459,370 - +0.0 459,401 + -1.5 459,401 + +0.2 459,401 + -0.5 459,401 + -0.1 459,402 - +1.5 459,402 - -2.1 459,402 - +0.5 459,408 + -0.8 459,534 - Pf1N1B4_6004 0.18 -0.8 459,571 + Pf1N1B4_6004 0.21 -0.7 459,814 + Pf1N1B4_6004 0.43 -0.5 459,814 + Pf1N1B4_6004 0.43 -1.5 459,814 + Pf1N1B4_6004 0.43 -0.5 460,029 + -2.3 460,082 + Pf1N1B4_6004 0.67 -3.1 460,124 + Pf1N1B4_6004 0.70 -2.5 460,124 + Pf1N1B4_6004 0.70 -2.0 460,124 + Pf1N1B4_6004 0.70 -1.5 460,125 - Pf1N1B4_6004 0.70 -0.3 460,131 + Pf1N1B4_6004 0.71 -1.0 460,131 + Pf1N1B4_6004 0.71 -0.5 460,132 - +0.5 460,162 + Pf1N1B4_6004 0.74 -2.0 460,256 + Pf1N1B4_6004 0.82 -1.5 460,256 + Pf1N1B4_6004 0.82 -1.8 460,299 + Pf1N1B4_6004 0.86 -0.4 460,360 + +0.0 460,399 + +0.0 460,400 - -0.5 460,433 + -1.8 460,433 + -0.8 460,435 + +0.1 460,768 + Pf1N1B4_6005 0.24 -0.8 460,772 + Pf1N1B4_6005 0.25 +0.5 461,093 + Pf1N1B4_6005 0.49 +1.5
Or see this region's nucleotide sequence