Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5976

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5975 and Pf1N1B4_5976 are separated by 115 nucleotidesPf1N1B4_5976 and Pf1N1B4_5977 are separated by 71 nucleotides Pf1N1B4_5975 - 2-methylaconitate racemase, at 422,673 to 423,701 _5975 Pf1N1B4_5976 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 423,817 to 424,734 _5976 Pf1N1B4_5977 - Transporter, MFS superfamily, at 424,806 to 426,203 _5977 Position (kb) 423 424 425Strain fitness (log2 ratio) -2 -1 0 1 2at 423.335 kb on + strandat 423.335 kb on + strand, within Pf1N1B4_5975at 423.336 kb on - strand, within Pf1N1B4_5975at 423.373 kb on + strand, within Pf1N1B4_5975at 423.374 kb on - strand, within Pf1N1B4_5975at 423.589 kb on - strand, within Pf1N1B4_5975at 423.589 kb on - strand, within Pf1N1B4_5975at 423.739 kb on - strandat 423.739 kb on - strandat 423.831 kb on + strandat 423.832 kb on - strandat 424.192 kb on + strand, within Pf1N1B4_5976at 424.228 kb on + strandat 424.228 kb on + strand, within Pf1N1B4_5976at 424.246 kb on + strand, within Pf1N1B4_5976at 424.528 kb on + strand, within Pf1N1B4_5976at 424.529 kb on - strand, within Pf1N1B4_5976at 424.820 kb on - strandat 424.862 kb on - strandat 425.598 kb on + strand, within Pf1N1B4_5977at 425.599 kb on - strandat 425.599 kb on - strand, within Pf1N1B4_5977at 425.625 kb on + strand, within Pf1N1B4_5977at 425.721 kb on + strand, within Pf1N1B4_5977

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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423,335 + -0.1
423,335 + Pf1N1B4_5975 0.64 +0.3
423,336 - Pf1N1B4_5975 0.64 -2.1
423,373 + Pf1N1B4_5975 0.68 +0.1
423,374 - Pf1N1B4_5975 0.68 -1.1
423,589 - Pf1N1B4_5975 0.89 -0.0
423,589 - Pf1N1B4_5975 0.89 -1.8
423,739 - +0.3
423,739 - +1.3
423,831 + -1.3
423,832 - -0.1
424,192 + Pf1N1B4_5976 0.41 -0.1
424,228 + -1.8
424,228 + Pf1N1B4_5976 0.45 +0.1
424,246 + Pf1N1B4_5976 0.47 +0.3
424,528 + Pf1N1B4_5976 0.77 +0.2
424,529 - Pf1N1B4_5976 0.78 +2.1
424,820 - +0.8
424,862 - +0.5
425,598 + Pf1N1B4_5977 0.57 +0.5
425,599 - -1.1
425,599 - Pf1N1B4_5977 0.57 +0.7
425,625 + Pf1N1B4_5977 0.59 +1.5
425,721 + Pf1N1B4_5977 0.65 +0.4

Or see this region's nucleotide sequence