Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5883 and Pf1N1B4_5884 are separated by 380 nucleotides Pf1N1B4_5884 and Pf1N1B4_5885 overlap by 4 nucleotides
Pf1N1B4_5883 - FIG00960892: hypothetical protein, at 325,237 to 326,934
_5883
Pf1N1B4_5884 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 327,315 to 328,667
_5884
Pf1N1B4_5885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 328,664 to 329,722
_5885
Position (kb) 327
328
329 Strain fitness (log2 ratio) -2
-1
0
1
2 at 326.393 kb on + strand, within Pf1N1B4_5883 at 326.394 kb on - strand at 326.426 kb on + strand, within Pf1N1B4_5883 at 326.426 kb on + strand, within Pf1N1B4_5883 at 326.426 kb on + strand, within Pf1N1B4_5883 at 326.427 kb on - strand, within Pf1N1B4_5883 at 326.676 kb on - strand at 326.676 kb on - strand, within Pf1N1B4_5883 at 326.836 kb on - strand at 326.883 kb on - strand at 326.897 kb on + strand at 326.898 kb on - strand at 327.068 kb on + strand at 327.286 kb on - strand at 327.328 kb on + strand at 327.342 kb on + strand at 327.355 kb on + strand at 327.356 kb on - strand at 327.879 kb on + strand, within Pf1N1B4_5884 at 327.880 kb on - strand at 328.121 kb on + strand, within Pf1N1B4_5884 at 328.266 kb on - strand, within Pf1N1B4_5884 at 328.381 kb on - strand, within Pf1N1B4_5884 at 328.417 kb on - strand, within Pf1N1B4_5884 at 328.597 kb on + strand at 328.597 kb on + strand at 328.598 kb on - strand at 328.671 kb on + strand at 328.671 kb on + strand at 328.744 kb on - strand at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.938 kb on - strand, within Pf1N1B4_5885 at 328.962 kb on - strand, within Pf1N1B4_5885 at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.036 kb on + strand at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.121 kb on - strand, within Pf1N1B4_5885 at 329.162 kb on + strand at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.481 kb on - strand, within Pf1N1B4_5885 at 329.660 kb on + strand at 329.661 kb on - strand at 329.663 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 326,393 + Pf1N1B4_5883 0.68 -2.3 326,394 - +0.1 326,426 + Pf1N1B4_5883 0.70 +1.1 326,426 + Pf1N1B4_5883 0.70 -0.2 326,426 + Pf1N1B4_5883 0.70 -0.3 326,427 - Pf1N1B4_5883 0.70 +0.1 326,676 - -0.1 326,676 - Pf1N1B4_5883 0.85 +0.5 326,836 - -1.1 326,883 - +0.1 326,897 + +2.3 326,898 - -1.3 327,068 + -1.1 327,286 - +0.1 327,328 + -0.6 327,342 + -2.5 327,355 + -0.3 327,356 - -0.1 327,879 + Pf1N1B4_5884 0.42 -1.5 327,880 - -1.5 328,121 + Pf1N1B4_5884 0.60 -0.1 328,266 - Pf1N1B4_5884 0.70 -0.5 328,381 - Pf1N1B4_5884 0.79 -0.1 328,417 - Pf1N1B4_5884 0.81 -1.4 328,597 + +0.5 328,597 + -1.2 328,598 - -1.1 328,671 + +1.4 328,671 + -0.5 328,744 - -0.9 328,773 - Pf1N1B4_5885 0.10 +0.8 328,773 - Pf1N1B4_5885 0.10 -2.1 328,938 - Pf1N1B4_5885 0.26 -0.2 328,962 - Pf1N1B4_5885 0.28 +1.8 329,036 + Pf1N1B4_5885 0.35 +0.6 329,036 + -0.5 329,036 + Pf1N1B4_5885 0.35 -1.1 329,121 - Pf1N1B4_5885 0.43 -1.2 329,162 + +0.5 329,162 + Pf1N1B4_5885 0.47 -0.4 329,162 + Pf1N1B4_5885 0.47 -1.0 329,163 - Pf1N1B4_5885 0.47 -0.8 329,163 - Pf1N1B4_5885 0.47 -1.2 329,324 + Pf1N1B4_5885 0.62 -0.4 329,324 + Pf1N1B4_5885 0.62 +0.5 329,325 - Pf1N1B4_5885 0.62 -1.0 329,325 - Pf1N1B4_5885 0.62 -0.7 329,442 - Pf1N1B4_5885 0.73 +0.3 329,442 - Pf1N1B4_5885 0.73 +0.5 329,481 - Pf1N1B4_5885 0.77 -0.5 329,660 + +0.5 329,661 - -1.5 329,663 + +0.5
Or see this region's nucleotide sequence