Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5859

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5858 and Pf1N1B4_5859 are separated by 229 nucleotidesPf1N1B4_5859 and Pf1N1B4_5860 are separated by 213 nucleotides Pf1N1B4_5858 - carboxyphosphonoenolpyruvate phosphonomutase, putative, at 291,317 to 292,186 _5858 Pf1N1B4_5859 - Glutamate carboxypeptidase (EC 3.4.17.11), at 292,416 to 293,657 _5859 Pf1N1B4_5860 - Two-component response regulator, at 293,871 to 294,806 _5860 Position (kb) 292 293 294Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 291.931 kb on - strand, within Pf1N1B4_5858at 291.950 kb on + strand, within Pf1N1B4_5858at 291.951 kb on - strand, within Pf1N1B4_5858at 292.028 kb on + strand, within Pf1N1B4_5858at 292.029 kb on - strand, within Pf1N1B4_5858at 292.053 kb on - strand, within Pf1N1B4_5858at 292.053 kb on - strand, within Pf1N1B4_5858at 292.123 kb on + strandat 292.123 kb on + strandat 292.123 kb on + strandat 292.123 kb on + strandat 292.123 kb on + strandat 292.124 kb on - strandat 292.124 kb on - strandat 292.124 kb on - strandat 292.124 kb on - strandat 292.124 kb on - strandat 292.124 kb on - strandat 292.124 kb on - strandat 292.127 kb on + strandat 292.127 kb on + strandat 292.127 kb on + strandat 292.128 kb on - strandat 292.128 kb on - strandat 292.145 kb on + strandat 292.145 kb on + strandat 292.145 kb on + strandat 292.145 kb on + strandat 292.146 kb on - strandat 292.146 kb on - strandat 292.146 kb on - strandat 292.146 kb on - strandat 292.166 kb on + strandat 292.166 kb on + strandat 292.166 kb on + strandat 292.166 kb on + strandat 292.166 kb on + strandat 292.167 kb on - strandat 292.168 kb on + strandat 292.168 kb on + strandat 292.168 kb on + strandat 292.168 kb on + strandat 292.169 kb on - strandat 292.169 kb on - strandat 292.169 kb on - strandat 292.169 kb on - strandat 292.169 kb on - strandat 292.268 kb on + strandat 292.268 kb on + strandat 292.269 kb on - strandat 292.269 kb on - strandat 292.457 kb on - strandat 292.503 kb on - strandat 292.503 kb on - strandat 292.508 kb on + strandat 292.509 kb on - strandat 292.695 kb on - strand, within Pf1N1B4_5859at 292.701 kb on - strandat 293.067 kb on + strand, within Pf1N1B4_5859at 293.068 kb on - strand, within Pf1N1B4_5859at 293.200 kb on + strand, within Pf1N1B4_5859at 293.200 kb on + strand, within Pf1N1B4_5859at 293.200 kb on + strand, within Pf1N1B4_5859at 293.200 kb on + strand, within Pf1N1B4_5859at 293.200 kb on + strand, within Pf1N1B4_5859at 293.200 kb on + strand, within Pf1N1B4_5859at 293.201 kb on - strandat 293.201 kb on - strand, within Pf1N1B4_5859at 293.534 kb on - strandat 293.695 kb on + strandat 293.695 kb on + strandat 293.793 kb on + strandat 293.793 kb on + strandat 294.061 kb on + strand, within Pf1N1B4_5860at 294.198 kb on + strand, within Pf1N1B4_5860at 294.199 kb on - strand, within Pf1N1B4_5860at 294.251 kb on + strand, within Pf1N1B4_5860at 294.252 kb on - strand, within Pf1N1B4_5860at 294.499 kb on + strand, within Pf1N1B4_5860at 294.562 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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291,931 - Pf1N1B4_5858 0.71 -3.0
291,950 + Pf1N1B4_5858 0.73 +2.1
291,951 - Pf1N1B4_5858 0.73 +0.2
292,028 + Pf1N1B4_5858 0.82 +0.2
292,029 - Pf1N1B4_5858 0.82 +1.5
292,053 - Pf1N1B4_5858 0.85 -0.5
292,053 - Pf1N1B4_5858 0.85 +0.0
292,123 + -0.6
292,123 + +0.5
292,123 + -3.4
292,123 + -2.5
292,123 + +2.1
292,124 - +0.9
292,124 - +0.1
292,124 - +0.1
292,124 - -1.5
292,124 - +0.5
292,124 - -2.3
292,124 - -0.3
292,127 + -0.1
292,127 + +0.5
292,127 + -1.5
292,128 - -1.5
292,128 - -0.5
292,145 + -0.8
292,145 + +1.6
292,145 + -2.3
292,145 + +0.2
292,146 - -0.3
292,146 - -2.5
292,146 - +0.5
292,146 - -0.9
292,166 + +0.5
292,166 + -0.7
292,166 + -0.8
292,166 + +0.2
292,166 + -2.5
292,167 - -0.8
292,168 + -0.2
292,168 + -1.8
292,168 + -2.3
292,168 + +0.5
292,169 - +0.9
292,169 - +0.3
292,169 - +0.1
292,169 - +0.9
292,169 - -1.1
292,268 + -1.1
292,268 + +0.3
292,269 - -0.1
292,269 - -0.2
292,457 - -2.3
292,503 - +0.5
292,503 - +0.1
292,508 + -1.5
292,509 - -0.5
292,695 - Pf1N1B4_5859 0.22 -3.0
292,701 - -0.5
293,067 + Pf1N1B4_5859 0.52 -1.5
293,068 - Pf1N1B4_5859 0.52 -1.5
293,200 + Pf1N1B4_5859 0.63 -1.2
293,200 + Pf1N1B4_5859 0.63 -0.5
293,200 + Pf1N1B4_5859 0.63 -2.0
293,200 + Pf1N1B4_5859 0.63 +0.5
293,200 + Pf1N1B4_5859 0.63 -1.6
293,200 + Pf1N1B4_5859 0.63 -1.1
293,201 - -0.1
293,201 - Pf1N1B4_5859 0.63 +1.7
293,534 - -0.4
293,695 + -0.1
293,695 + +0.5
293,793 + -1.8
293,793 + -0.2
294,061 + Pf1N1B4_5860 0.20 +0.3
294,198 + Pf1N1B4_5860 0.35 -2.3
294,199 - Pf1N1B4_5860 0.35 +1.1
294,251 + Pf1N1B4_5860 0.41 +1.3
294,252 - Pf1N1B4_5860 0.41 -0.5
294,499 + Pf1N1B4_5860 0.67 -0.8
294,562 - +0.1

Or see this region's nucleotide sequence