Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5858 and Pf1N1B4_5859 are separated by 229 nucleotides Pf1N1B4_5859 and Pf1N1B4_5860 are separated by 213 nucleotides
Pf1N1B4_5858 - carboxyphosphonoenolpyruvate phosphonomutase, putative, at 291,317 to 292,186
_5858
Pf1N1B4_5859 - Glutamate carboxypeptidase (EC 3.4.17.11), at 292,416 to 293,657
_5859
Pf1N1B4_5860 - Two-component response regulator, at 293,871 to 294,806
_5860
Position (kb)
292
293
294 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 291.931 kb on - strand, within Pf1N1B4_5858 at 291.950 kb on + strand, within Pf1N1B4_5858 at 291.951 kb on - strand, within Pf1N1B4_5858 at 292.028 kb on + strand, within Pf1N1B4_5858 at 292.029 kb on - strand, within Pf1N1B4_5858 at 292.053 kb on - strand, within Pf1N1B4_5858 at 292.053 kb on - strand, within Pf1N1B4_5858 at 292.123 kb on + strand at 292.123 kb on + strand at 292.123 kb on + strand at 292.123 kb on + strand at 292.123 kb on + strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.124 kb on - strand at 292.127 kb on + strand at 292.127 kb on + strand at 292.127 kb on + strand at 292.128 kb on - strand at 292.128 kb on - strand at 292.145 kb on + strand at 292.145 kb on + strand at 292.145 kb on + strand at 292.145 kb on + strand at 292.146 kb on - strand at 292.146 kb on - strand at 292.146 kb on - strand at 292.146 kb on - strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.166 kb on + strand at 292.167 kb on - strand at 292.168 kb on + strand at 292.168 kb on + strand at 292.168 kb on + strand at 292.168 kb on + strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.169 kb on - strand at 292.268 kb on + strand at 292.268 kb on + strand at 292.269 kb on - strand at 292.269 kb on - strand at 292.457 kb on - strand at 292.503 kb on - strand at 292.503 kb on - strand at 292.508 kb on + strand at 292.509 kb on - strand at 292.695 kb on - strand, within Pf1N1B4_5859 at 292.701 kb on - strand at 293.067 kb on + strand, within Pf1N1B4_5859 at 293.068 kb on - strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.201 kb on - strand at 293.201 kb on - strand, within Pf1N1B4_5859 at 293.534 kb on - strand at 293.695 kb on + strand at 293.695 kb on + strand at 293.793 kb on + strand at 293.793 kb on + strand at 294.061 kb on + strand, within Pf1N1B4_5860 at 294.198 kb on + strand, within Pf1N1B4_5860 at 294.199 kb on - strand, within Pf1N1B4_5860 at 294.251 kb on + strand, within Pf1N1B4_5860 at 294.252 kb on - strand, within Pf1N1B4_5860 at 294.499 kb on + strand, within Pf1N1B4_5860 at 294.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 291,931 - Pf1N1B4_5858 0.71 -3.0 291,950 + Pf1N1B4_5858 0.73 +2.1 291,951 - Pf1N1B4_5858 0.73 +0.2 292,028 + Pf1N1B4_5858 0.82 +0.2 292,029 - Pf1N1B4_5858 0.82 +1.5 292,053 - Pf1N1B4_5858 0.85 -0.5 292,053 - Pf1N1B4_5858 0.85 +0.0 292,123 + -0.6 292,123 + +0.5 292,123 + -3.4 292,123 + -2.5 292,123 + +2.1 292,124 - +0.9 292,124 - +0.1 292,124 - +0.1 292,124 - -1.5 292,124 - +0.5 292,124 - -2.3 292,124 - -0.3 292,127 + -0.1 292,127 + +0.5 292,127 + -1.5 292,128 - -1.5 292,128 - -0.5 292,145 + -0.8 292,145 + +1.6 292,145 + -2.3 292,145 + +0.2 292,146 - -0.3 292,146 - -2.5 292,146 - +0.5 292,146 - -0.9 292,166 + +0.5 292,166 + -0.7 292,166 + -0.8 292,166 + +0.2 292,166 + -2.5 292,167 - -0.8 292,168 + -0.2 292,168 + -1.8 292,168 + -2.3 292,168 + +0.5 292,169 - +0.9 292,169 - +0.3 292,169 - +0.1 292,169 - +0.9 292,169 - -1.1 292,268 + -1.1 292,268 + +0.3 292,269 - -0.1 292,269 - -0.2 292,457 - -2.3 292,503 - +0.5 292,503 - +0.1 292,508 + -1.5 292,509 - -0.5 292,695 - Pf1N1B4_5859 0.22 -3.0 292,701 - -0.5 293,067 + Pf1N1B4_5859 0.52 -1.5 293,068 - Pf1N1B4_5859 0.52 -1.5 293,200 + Pf1N1B4_5859 0.63 -1.2 293,200 + Pf1N1B4_5859 0.63 -0.5 293,200 + Pf1N1B4_5859 0.63 -2.0 293,200 + Pf1N1B4_5859 0.63 +0.5 293,200 + Pf1N1B4_5859 0.63 -1.6 293,200 + Pf1N1B4_5859 0.63 -1.1 293,201 - -0.1 293,201 - Pf1N1B4_5859 0.63 +1.7 293,534 - -0.4 293,695 + -0.1 293,695 + +0.5 293,793 + -1.8 293,793 + -0.2 294,061 + Pf1N1B4_5860 0.20 +0.3 294,198 + Pf1N1B4_5860 0.35 -2.3 294,199 - Pf1N1B4_5860 0.35 +1.1 294,251 + Pf1N1B4_5860 0.41 +1.3 294,252 - Pf1N1B4_5860 0.41 -0.5 294,499 + Pf1N1B4_5860 0.67 -0.8 294,562 - +0.1
Or see this region's nucleotide sequence