Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5845 and Pf1N1B4_5846 are separated by 117 nucleotides Pf1N1B4_5846 and Pf1N1B4_5847 are separated by 101 nucleotides
Pf1N1B4_5845 - GMP synthase - Glutamine amidotransferase domain, at 279,680 to 280,321
_5845
Pf1N1B4_5846 - Rhodanese-related sulfurtransferase, at 280,439 to 280,873
_5846
Pf1N1B4_5847 - transcriptional activator FtrA, at 280,975 to 281,949
_5847
Position (kb) 280
281 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 279.483 kb on + strand at 279.483 kb on + strand at 279.484 kb on - strand at 279.484 kb on - strand at 279.487 kb on + strand at 279.489 kb on + strand at 279.516 kb on - strand at 279.840 kb on - strand, within Pf1N1B4_5845 at 280.369 kb on - strand at 280.369 kb on - strand at 280.369 kb on - strand at 280.403 kb on + strand at 280.404 kb on - strand at 280.612 kb on - strand, within Pf1N1B4_5846 at 280.774 kb on - strand, within Pf1N1B4_5846 at 280.880 kb on + strand at 280.880 kb on + strand at 280.881 kb on - strand at 280.914 kb on - strand at 280.914 kb on - strand at 281.011 kb on + strand at 281.012 kb on - strand at 281.197 kb on - strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.402 kb on + strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand, within Pf1N1B4_5847 at 281.403 kb on - strand at 281.425 kb on + strand, within Pf1N1B4_5847 at 281.425 kb on + strand at 281.425 kb on + strand, within Pf1N1B4_5847
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 279,483 + +1.2 279,483 + -3.2 279,484 - +0.3 279,484 - +0.3 279,487 + -0.6 279,489 + +0.4 279,516 - -0.9 279,840 - Pf1N1B4_5845 0.25 +0.5 280,369 - -1.1 280,369 - +0.1 280,369 - +0.5 280,403 + +0.9 280,404 - +1.0 280,612 - Pf1N1B4_5846 0.40 +0.7 280,774 - Pf1N1B4_5846 0.77 +1.1 280,880 + +1.1 280,880 + +0.1 280,881 - -1.1 280,914 - -1.1 280,914 - +0.1 281,011 + -0.6 281,012 - +0.1 281,197 - Pf1N1B4_5847 0.23 +2.8 281,402 + Pf1N1B4_5847 0.44 -0.5 281,402 + Pf1N1B4_5847 0.44 +1.1 281,402 + Pf1N1B4_5847 0.44 -0.7 281,402 + Pf1N1B4_5847 0.44 +0.5 281,402 + Pf1N1B4_5847 0.44 -0.5 281,403 - Pf1N1B4_5847 0.44 -0.7 281,403 - Pf1N1B4_5847 0.44 +0.8 281,403 - Pf1N1B4_5847 0.44 -1.5 281,403 - +0.5 281,425 + Pf1N1B4_5847 0.46 -0.5 281,425 + -0.8 281,425 + Pf1N1B4_5847 0.46 +0.9
Or see this region's nucleotide sequence