Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_581 and Pf1N1B4_582 are separated by 157 nucleotides Pf1N1B4_582 and Pf1N1B4_583 are separated by 201 nucleotides Pf1N1B4_583 and Pf1N1B4_584 are separated by 25 nucleotides
Pf1N1B4_581 - membrane protein, putative, at 672,568 to 674,556
_581
Pf1N1B4_582 - FIG00959696: hypothetical protein, at 674,714 to 675,181
_582
Pf1N1B4_583 - FIG00957748: hypothetical protein, at 675,383 to 675,622
_583
Pf1N1B4_584 - Isopenicillin N epimerase (EC 5.1.1.17), at 675,648 to 676,829
_584
Position (kb) 675
676 Strain fitness (log2 ratio) -2
-1
0
1
2 at 674.410 kb on - strand at 674.558 kb on + strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.559 kb on - strand at 674.634 kb on + strand at 674.656 kb on - strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.715 kb on + strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.716 kb on - strand at 674.781 kb on + strand, within Pf1N1B4_582 at 674.850 kb on + strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 674.851 kb on - strand, within Pf1N1B4_582 at 675.056 kb on - strand, within Pf1N1B4_582 at 675.056 kb on - strand, within Pf1N1B4_582 at 675.056 kb on - strand, within Pf1N1B4_582 at 675.149 kb on + strand at 675.150 kb on - strand at 675.150 kb on - strand at 675.166 kb on + strand at 675.167 kb on - strand at 675.167 kb on - strand at 675.169 kb on + strand at 675.215 kb on + strand at 675.215 kb on + strand at 675.215 kb on + strand at 675.216 kb on - strand at 675.216 kb on - strand at 675.220 kb on - strand at 675.441 kb on - strand, within Pf1N1B4_583 at 675.441 kb on - strand, within Pf1N1B4_583 at 675.441 kb on - strand, within Pf1N1B4_583 at 675.441 kb on - strand, within Pf1N1B4_583 at 675.784 kb on - strand, within Pf1N1B4_584 at 675.960 kb on + strand, within Pf1N1B4_584 at 675.960 kb on + strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand, within Pf1N1B4_584 at 675.961 kb on - strand at 675.995 kb on - strand at 676.008 kb on + strand, within Pf1N1B4_584 at 676.365 kb on + strand, within Pf1N1B4_584 at 676.365 kb on + strand, within Pf1N1B4_584 at 676.365 kb on + strand, within Pf1N1B4_584 at 676.366 kb on - strand, within Pf1N1B4_584 at 676.366 kb on - strand, within Pf1N1B4_584 at 676.366 kb on - strand, within Pf1N1B4_584 at 676.551 kb on + strand, within Pf1N1B4_584 at 676.551 kb on + strand, within Pf1N1B4_584 at 676.552 kb on - strand, within Pf1N1B4_584
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 674,410 - -0.2 674,558 + -1.7 674,559 - +0.9 674,559 - -0.3 674,559 - -1.2 674,559 - -1.2 674,559 - -0.1 674,634 + +0.3 674,656 - -0.7 674,715 + -0.6 674,715 + +0.9 674,715 + +0.3 674,715 + +0.1 674,715 + +0.5 674,716 - +0.8 674,716 - -0.1 674,716 - +0.9 674,716 - +0.1 674,716 - +0.2 674,716 - +0.3 674,716 - -1.4 674,716 - -0.7 674,716 - +0.2 674,781 + Pf1N1B4_582 0.14 -0.7 674,850 + Pf1N1B4_582 0.29 +1.5 674,851 - Pf1N1B4_582 0.29 +0.2 674,851 - Pf1N1B4_582 0.29 +0.6 674,851 - Pf1N1B4_582 0.29 -0.0 674,851 - Pf1N1B4_582 0.29 +1.9 674,851 - Pf1N1B4_582 0.29 -0.4 674,851 - Pf1N1B4_582 0.29 -0.4 675,056 - Pf1N1B4_582 0.73 -2.1 675,056 - Pf1N1B4_582 0.73 -1.9 675,056 - Pf1N1B4_582 0.73 +0.3 675,149 + +1.9 675,150 - -0.3 675,150 - -0.1 675,166 + +0.9 675,167 - -0.1 675,167 - +1.5 675,169 + -1.7 675,215 + +1.9 675,215 + +0.5 675,215 + +0.5 675,216 - -0.5 675,216 - -1.4 675,220 - +0.3 675,441 - Pf1N1B4_583 0.24 -0.7 675,441 - Pf1N1B4_583 0.24 -1.8 675,441 - Pf1N1B4_583 0.24 +0.3 675,441 - Pf1N1B4_583 0.24 -0.1 675,784 - Pf1N1B4_584 0.12 +0.2 675,960 + Pf1N1B4_584 0.26 +0.6 675,960 + Pf1N1B4_584 0.26 +0.6 675,961 - Pf1N1B4_584 0.26 -1.7 675,961 - Pf1N1B4_584 0.26 -1.9 675,961 - Pf1N1B4_584 0.26 +0.3 675,961 - Pf1N1B4_584 0.26 -1.9 675,961 - +0.9 675,995 - -0.1 676,008 + Pf1N1B4_584 0.30 -0.7 676,365 + Pf1N1B4_584 0.61 +0.7 676,365 + Pf1N1B4_584 0.61 -0.4 676,365 + Pf1N1B4_584 0.61 -0.7 676,366 - Pf1N1B4_584 0.61 +1.5 676,366 - Pf1N1B4_584 0.61 -0.1 676,366 - Pf1N1B4_584 0.61 -0.1 676,551 + Pf1N1B4_584 0.76 -0.1 676,551 + Pf1N1B4_584 0.76 +0.6 676,552 - Pf1N1B4_584 0.76 -1.7
Or see this region's nucleotide sequence