Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5812

Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5809 and Pf1N1B4_5810 are separated by 247 nucleotidesPf1N1B4_5810 and Pf1N1B4_5811 are separated by 67 nucleotidesPf1N1B4_5811 and Pf1N1B4_5812 overlap by 4 nucleotidesPf1N1B4_5812 and Pf1N1B4_5813 are separated by 393 nucleotides Pf1N1B4_5809 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 243,057 to 244,004 _5809 Pf1N1B4_5810 - hypothetical protein, at 244,252 to 244,410 _5810 Pf1N1B4_5811 - FIG00958502: hypothetical protein, at 244,478 to 244,792 _5811 Pf1N1B4_5812 - FIG00955248: hypothetical protein, at 244,789 to 245,487 _5812 Pf1N1B4_5813 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 245,881 to 246,756 _5813 Position (kb) 244 245 246Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 243.844 kb on + strand, within Pf1N1B4_5809at 243.895 kb on + strand, within Pf1N1B4_5809at 243.898 kb on + strand, within Pf1N1B4_5809at 243.940 kb on + strandat 243.988 kb on + strandat 244.144 kb on + strandat 244.176 kb on + strandat 244.176 kb on + strandat 244.177 kb on - strandat 244.222 kb on - strandat 244.454 kb on + strandat 244.454 kb on + strandat 244.454 kb on + strandat 244.455 kb on - strandat 244.548 kb on + strand, within Pf1N1B4_5811at 244.548 kb on + strand, within Pf1N1B4_5811at 244.548 kb on + strand, within Pf1N1B4_5811at 244.548 kb on + strand, within Pf1N1B4_5811at 244.548 kb on + strand, within Pf1N1B4_5811at 244.549 kb on - strandat 244.549 kb on - strand, within Pf1N1B4_5811at 244.549 kb on - strand, within Pf1N1B4_5811at 244.549 kb on - strand, within Pf1N1B4_5811at 244.549 kb on - strand, within Pf1N1B4_5811at 244.733 kb on + strand, within Pf1N1B4_5811at 244.734 kb on - strand, within Pf1N1B4_5811at 244.734 kb on - strand, within Pf1N1B4_5811at 244.734 kb on - strand, within Pf1N1B4_5811at 244.734 kb on - strand, within Pf1N1B4_5811at 244.734 kb on - strand, within Pf1N1B4_5811at 244.805 kb on + strandat 244.805 kb on + strandat 244.806 kb on - strandat 244.806 kb on - strandat 244.822 kb on - strandat 245.067 kb on - strand, within Pf1N1B4_5812at 245.094 kb on - strand, within Pf1N1B4_5812at 245.094 kb on - strand, within Pf1N1B4_5812at 245.211 kb on + strand, within Pf1N1B4_5812at 245.212 kb on - strand, within Pf1N1B4_5812at 245.212 kb on - strand, within Pf1N1B4_5812at 245.212 kb on - strand, within Pf1N1B4_5812at 245.212 kb on - strand, within Pf1N1B4_5812at 245.487 kb on + strandat 245.488 kb on - strandat 245.496 kb on - strandat 245.615 kb on - strandat 245.640 kb on + strandat 245.641 kb on - strandat 245.641 kb on - strandat 245.641 kb on - strandat 245.806 kb on + strandat 245.834 kb on + strandat 245.834 kb on + strandat 245.834 kb on + strandat 245.834 kb on + strandat 245.834 kb on + strandat 245.835 kb on - strandat 245.898 kb on + strandat 245.899 kb on - strandat 245.899 kb on - strandat 245.899 kb on - strandat 246.197 kb on - strand, within Pf1N1B4_5813at 246.199 kb on + strand, within Pf1N1B4_5813at 246.199 kb on + strand, within Pf1N1B4_5813at 246.458 kb on + strand, within Pf1N1B4_5813

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM
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243,844 + Pf1N1B4_5809 0.83 -0.3
243,895 + Pf1N1B4_5809 0.88 -0.5
243,898 + Pf1N1B4_5809 0.89 -0.1
243,940 + +0.3
243,988 + -0.5
244,144 + -0.3
244,176 + +1.2
244,176 + -0.2
244,177 - +0.9
244,222 - +0.2
244,454 + -0.7
244,454 + +0.5
244,454 + -0.3
244,455 - +1.5
244,548 + Pf1N1B4_5811 0.22 -0.5
244,548 + Pf1N1B4_5811 0.22 +0.8
244,548 + Pf1N1B4_5811 0.22 -0.4
244,548 + Pf1N1B4_5811 0.22 -2.3
244,548 + Pf1N1B4_5811 0.22 -0.8
244,549 - -1.1
244,549 - Pf1N1B4_5811 0.23 +0.0
244,549 - Pf1N1B4_5811 0.23 -0.9
244,549 - Pf1N1B4_5811 0.23 +0.1
244,549 - Pf1N1B4_5811 0.23 +0.8
244,733 + Pf1N1B4_5811 0.81 +0.5
244,734 - Pf1N1B4_5811 0.81 +0.5
244,734 - Pf1N1B4_5811 0.81 +0.2
244,734 - Pf1N1B4_5811 0.81 +1.7
244,734 - Pf1N1B4_5811 0.81 -0.3
244,734 - Pf1N1B4_5811 0.81 +1.8
244,805 + -0.2
244,805 + -1.1
244,806 - +0.1
244,806 - -1.2
244,822 - -0.5
245,067 - Pf1N1B4_5812 0.40 -0.7
245,094 - Pf1N1B4_5812 0.44 -0.1
245,094 - Pf1N1B4_5812 0.44 -2.1
245,211 + Pf1N1B4_5812 0.60 -2.8
245,212 - Pf1N1B4_5812 0.61 +0.5
245,212 - Pf1N1B4_5812 0.61 -0.5
245,212 - Pf1N1B4_5812 0.61 -1.3
245,212 - Pf1N1B4_5812 0.61 -1.1
245,487 + -1.2
245,488 - +1.1
245,496 - +0.6
245,615 - -2.5
245,640 + -0.7
245,641 - +0.5
245,641 - -0.5
245,641 - +0.5
245,806 + -0.3
245,834 + -0.9
245,834 + -1.9
245,834 + -0.4
245,834 + +0.9
245,834 + -2.1
245,835 - +0.1
245,898 + -0.7
245,899 - -0.8
245,899 - +0.5
245,899 - -0.8
246,197 - Pf1N1B4_5813 0.36 -1.5
246,199 + Pf1N1B4_5813 0.36 +1.1
246,199 + Pf1N1B4_5813 0.36 +1.1
246,458 + Pf1N1B4_5813 0.66 +1.1

Or see this region's nucleotide sequence