Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5797 and Pf1N1B4_5798 are separated by 191 nucleotides Pf1N1B4_5798 and Pf1N1B4_5799 are separated by 10 nucleotides Pf1N1B4_5799 and Pf1N1B4_5800 overlap by 4 nucleotides
Pf1N1B4_5797 - 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase (EC 1.3.1.25), at 232,800 to 233,573
_5797
Pf1N1B4_5798 - 2-chlorobenzoate 1,2-dioxygenase reductase component, at 233,765 to 234,778
_5798
Pf1N1B4_5799 - Benzoate 1,2-dioxygenase beta subunit (EC 1.14.12.10), at 234,789 to 235,277
_5799
Pf1N1B4_5800 - Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10), at 235,274 to 236,638
_5800
Position (kb) 233
234
235 Strain fitness (log2 ratio) -2
-1
0
1 at 232.846 kb on + strand at 232.847 kb on - strand at 232.847 kb on - strand at 232.847 kb on - strand at 232.847 kb on - strand at 233.380 kb on + strand, within Pf1N1B4_5797 at 233.409 kb on + strand, within Pf1N1B4_5797 at 233.409 kb on + strand, within Pf1N1B4_5797 at 233.410 kb on - strand, within Pf1N1B4_5797 at 233.577 kb on + strand at 233.578 kb on - strand at 233.605 kb on + strand at 233.606 kb on - strand at 233.606 kb on - strand at 233.628 kb on + strand at 233.629 kb on - strand at 233.685 kb on + strand at 233.797 kb on - strand at 233.874 kb on + strand, within Pf1N1B4_5798 at 233.928 kb on + strand, within Pf1N1B4_5798 at 234.171 kb on - strand, within Pf1N1B4_5798 at 234.175 kb on - strand, within Pf1N1B4_5798 at 234.175 kb on - strand, within Pf1N1B4_5798 at 234.357 kb on + strand at 234.357 kb on + strand, within Pf1N1B4_5798 at 234.358 kb on - strand, within Pf1N1B4_5798 at 234.358 kb on - strand, within Pf1N1B4_5798 at 234.460 kb on - strand, within Pf1N1B4_5798 at 234.591 kb on + strand at 234.591 kb on + strand, within Pf1N1B4_5798 at 234.591 kb on + strand, within Pf1N1B4_5798 at 234.592 kb on - strand, within Pf1N1B4_5798 at 234.800 kb on - strand at 234.824 kb on - strand at 234.824 kb on - strand at 234.824 kb on - strand at 234.890 kb on - strand, within Pf1N1B4_5799 at 234.890 kb on - strand, within Pf1N1B4_5799 at 234.907 kb on + strand, within Pf1N1B4_5799 at 234.907 kb on + strand, within Pf1N1B4_5799 at 234.907 kb on + strand, within Pf1N1B4_5799 at 234.907 kb on + strand, within Pf1N1B4_5799 at 234.908 kb on - strand, within Pf1N1B4_5799 at 235.186 kb on + strand at 235.268 kb on + strand at 235.268 kb on + strand at 235.269 kb on - strand at 235.269 kb on - strand at 235.269 kb on - strand at 235.347 kb on + strand at 235.347 kb on + strand at 235.348 kb on - strand at 235.632 kb on + strand, within Pf1N1B4_5800 at 235.633 kb on - strand, within Pf1N1B4_5800 at 235.633 kb on - strand, within Pf1N1B4_5800 at 235.707 kb on + strand, within Pf1N1B4_5800
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 232,846 + -0.4 232,847 - +0.4 232,847 - -2.4 232,847 - -0.1 232,847 - -1.9 233,380 + Pf1N1B4_5797 0.75 +0.1 233,409 + Pf1N1B4_5797 0.79 +1.1 233,409 + Pf1N1B4_5797 0.79 -0.6 233,410 - Pf1N1B4_5797 0.79 -0.6 233,577 + -0.6 233,578 - +0.9 233,605 + +0.9 233,606 - +0.4 233,606 - -0.8 233,628 + -1.3 233,629 - +0.4 233,685 + +0.6 233,797 - +1.2 233,874 + Pf1N1B4_5798 0.11 -0.3 233,928 + Pf1N1B4_5798 0.16 -0.3 234,171 - Pf1N1B4_5798 0.40 -1.3 234,175 - Pf1N1B4_5798 0.40 -2.1 234,175 - Pf1N1B4_5798 0.40 -1.7 234,357 + -0.1 234,357 + Pf1N1B4_5798 0.58 +0.4 234,358 - Pf1N1B4_5798 0.58 -0.8 234,358 - Pf1N1B4_5798 0.58 +0.9 234,460 - Pf1N1B4_5798 0.69 -1.6 234,591 + -0.6 234,591 + Pf1N1B4_5798 0.81 -0.7 234,591 + Pf1N1B4_5798 0.81 -1.0 234,592 - Pf1N1B4_5798 0.82 -1.6 234,800 - -0.5 234,824 - -0.0 234,824 - -0.9 234,824 - +1.1 234,890 - Pf1N1B4_5799 0.21 -0.5 234,890 - Pf1N1B4_5799 0.21 -0.1 234,907 + Pf1N1B4_5799 0.24 -1.4 234,907 + Pf1N1B4_5799 0.24 +1.0 234,907 + Pf1N1B4_5799 0.24 -2.5 234,907 + Pf1N1B4_5799 0.24 -0.3 234,908 - Pf1N1B4_5799 0.24 +1.0 235,186 + -1.1 235,268 + +0.5 235,268 + -0.5 235,269 - -1.5 235,269 - +0.1 235,269 - -1.2 235,347 + -1.1 235,347 + +0.5 235,348 - -2.1 235,632 + Pf1N1B4_5800 0.26 +0.2 235,633 - Pf1N1B4_5800 0.26 -0.9 235,633 - Pf1N1B4_5800 0.26 +0.7 235,707 + Pf1N1B4_5800 0.32 -0.9
Or see this region's nucleotide sequence