Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5767 and Pf1N1B4_5768 are separated by 1 nucleotides Pf1N1B4_5768 and Pf1N1B4_5769 overlap by 4 nucleotides Pf1N1B4_5769 and Pf1N1B4_5770 overlap by 8 nucleotides Pf1N1B4_5770 and Pf1N1B4_5771 overlap by 26 nucleotides
Pf1N1B4_5767 - Heme d1 biosynthesis protein NirF, at 205,503 to 206,681
_5767
Pf1N1B4_5768 - Heme d1 biosynthesis protein NirD, at 206,683 to 207,141
_5768
Pf1N1B4_5769 - Heme d1 biosynthesis protein NirL, at 207,138 to 207,653
_5769
Pf1N1B4_5770 - Heme d1 biosynthesis protein NirG, at 207,646 to 208,089
_5770
Pf1N1B4_5771 - Heme d1 biosynthesis protein NirH, at 208,064 to 208,579
_5771
Position (kb) 206
207
208 Strain fitness (log2 ratio) -2
-1
0
1 at 205.725 kb on + strand, within Pf1N1B4_5767 at 205.725 kb on + strand, within Pf1N1B4_5767 at 205.725 kb on + strand, within Pf1N1B4_5767 at 205.725 kb on + strand at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.726 kb on - strand, within Pf1N1B4_5767 at 205.796 kb on - strand, within Pf1N1B4_5767 at 205.860 kb on + strand, within Pf1N1B4_5767 at 205.861 kb on - strand, within Pf1N1B4_5767 at 205.861 kb on - strand, within Pf1N1B4_5767 at 205.861 kb on - strand, within Pf1N1B4_5767 at 205.861 kb on - strand at 205.861 kb on - strand, within Pf1N1B4_5767 at 206.094 kb on + strand, within Pf1N1B4_5767 at 206.095 kb on - strand, within Pf1N1B4_5767 at 206.127 kb on + strand, within Pf1N1B4_5767 at 206.127 kb on + strand, within Pf1N1B4_5767 at 206.127 kb on + strand, within Pf1N1B4_5767 at 206.127 kb on + strand, within Pf1N1B4_5767 at 206.128 kb on - strand, within Pf1N1B4_5767 at 206.756 kb on - strand, within Pf1N1B4_5768 at 206.756 kb on - strand, within Pf1N1B4_5768 at 206.964 kb on + strand, within Pf1N1B4_5768 at 206.965 kb on - strand, within Pf1N1B4_5768 at 206.968 kb on + strand at 206.968 kb on + strand, within Pf1N1B4_5768 at 206.969 kb on - strand, within Pf1N1B4_5768 at 206.987 kb on - strand, within Pf1N1B4_5768 at 207.344 kb on + strand, within Pf1N1B4_5769 at 207.344 kb on + strand, within Pf1N1B4_5769 at 207.439 kb on - strand, within Pf1N1B4_5769 at 207.472 kb on - strand, within Pf1N1B4_5769 at 207.929 kb on - strand, within Pf1N1B4_5770 at 207.929 kb on - strand, within Pf1N1B4_5770 at 208.060 kb on + strand at 208.060 kb on + strand at 208.061 kb on - strand at 208.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 205,725 + Pf1N1B4_5767 0.19 -0.3 205,725 + Pf1N1B4_5767 0.19 -0.8 205,725 + Pf1N1B4_5767 0.19 +0.4 205,725 + -1.6 205,726 - Pf1N1B4_5767 0.19 -1.1 205,726 - Pf1N1B4_5767 0.19 +0.4 205,726 - Pf1N1B4_5767 0.19 -2.7 205,726 - Pf1N1B4_5767 0.19 -0.6 205,726 - Pf1N1B4_5767 0.19 -0.6 205,726 - Pf1N1B4_5767 0.19 -0.9 205,796 - Pf1N1B4_5767 0.25 -0.6 205,860 + Pf1N1B4_5767 0.30 -0.3 205,861 - Pf1N1B4_5767 0.30 -0.6 205,861 - Pf1N1B4_5767 0.30 -0.6 205,861 - Pf1N1B4_5767 0.30 -0.8 205,861 - -0.6 205,861 - Pf1N1B4_5767 0.30 -1.4 206,094 + Pf1N1B4_5767 0.50 -0.1 206,095 - Pf1N1B4_5767 0.50 -2.6 206,127 + Pf1N1B4_5767 0.53 -2.6 206,127 + Pf1N1B4_5767 0.53 -2.3 206,127 + Pf1N1B4_5767 0.53 +0.9 206,127 + Pf1N1B4_5767 0.53 -0.1 206,128 - Pf1N1B4_5767 0.53 -0.4 206,756 - Pf1N1B4_5768 0.16 -2.1 206,756 - Pf1N1B4_5768 0.16 -0.9 206,964 + Pf1N1B4_5768 0.61 -0.9 206,965 - Pf1N1B4_5768 0.61 -0.8 206,968 + +0.0 206,968 + Pf1N1B4_5768 0.62 -2.1 206,969 - Pf1N1B4_5768 0.62 +0.8 206,987 - Pf1N1B4_5768 0.66 -0.1 207,344 + Pf1N1B4_5769 0.40 -0.6 207,344 + Pf1N1B4_5769 0.40 -0.9 207,439 - Pf1N1B4_5769 0.58 -0.9 207,472 - Pf1N1B4_5769 0.65 +0.7 207,929 - Pf1N1B4_5770 0.64 -0.2 207,929 - Pf1N1B4_5770 0.64 +0.4 208,060 + -0.7 208,060 + +0.3 208,061 - -0.6 208,076 - -2.1
Or see this region's nucleotide sequence