Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5674 and Pf1N1B4_5675 are separated by 150 nucleotides Pf1N1B4_5675 and Pf1N1B4_5676 are separated by 108 nucleotides Pf1N1B4_5676 and Pf1N1B4_5677 are separated by 218 nucleotides
Pf1N1B4_5674 - Strictosidine synthase precursor (EC 4.3.3.2), at 111,804 to 112,892
_5674
Pf1N1B4_5675 - hypothetical protein, at 113,043 to 113,303
_5675
Pf1N1B4_5676 - hypothetical protein, at 113,412 to 113,654
_5676
Pf1N1B4_5677 - putative exported protein, at 113,873 to 114,394
_5677
Position (kb)
113
114 Strain fitness (log2 ratio)
-1
0
1 at 112.064 kb on + strand at 112.614 kb on + strand, within Pf1N1B4_5674 at 112.614 kb on + strand, within Pf1N1B4_5674 at 112.615 kb on - strand at 112.615 kb on - strand, within Pf1N1B4_5674 at 112.663 kb on + strand, within Pf1N1B4_5674 at 112.663 kb on + strand, within Pf1N1B4_5674 at 112.788 kb on + strand at 112.788 kb on + strand at 112.788 kb on + strand at 112.788 kb on + strand at 112.815 kb on + strand at 112.816 kb on - strand at 112.830 kb on + strand at 112.830 kb on + strand at 112.890 kb on + strand at 112.891 kb on - strand at 112.891 kb on - strand at 113.055 kb on + strand at 113.056 kb on - strand at 113.056 kb on - strand at 113.056 kb on - strand at 113.142 kb on + strand, within Pf1N1B4_5675 at 113.143 kb on - strand, within Pf1N1B4_5675 at 113.143 kb on - strand, within Pf1N1B4_5675 at 113.228 kb on - strand, within Pf1N1B4_5675 at 113.356 kb on - strand at 113.360 kb on - strand at 113.406 kb on - strand at 113.551 kb on + strand, within Pf1N1B4_5676 at 113.552 kb on - strand, within Pf1N1B4_5676 at 113.848 kb on + strand at 113.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 112,064 + +0.4 112,614 + Pf1N1B4_5674 0.74 -1.7 112,614 + Pf1N1B4_5674 0.74 -0.7 112,615 - +0.4 112,615 - Pf1N1B4_5674 0.74 +0.2 112,663 + Pf1N1B4_5674 0.79 -0.9 112,663 + Pf1N1B4_5674 0.79 -0.9 112,788 + -0.9 112,788 + +0.0 112,788 + +1.3 112,788 + -0.7 112,815 + -1.3 112,816 - -1.6 112,830 + +0.0 112,830 + +0.4 112,890 + -0.1 112,891 - +0.4 112,891 - -1.0 113,055 + -0.9 113,056 - -0.0 113,056 - +0.0 113,056 - +0.4 113,142 + Pf1N1B4_5675 0.38 -0.7 113,143 - Pf1N1B4_5675 0.38 -1.6 113,143 - Pf1N1B4_5675 0.38 -0.3 113,228 - Pf1N1B4_5675 0.71 -1.3 113,356 - -0.5 113,360 - -0.0 113,406 - -0.2 113,551 + Pf1N1B4_5676 0.57 +0.0 113,552 - Pf1N1B4_5676 0.58 +1.7 113,848 + -0.6 113,849 - -0.6
Or see this region's nucleotide sequence